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European Archives of Oto-Rhino-Laryngology

, Volume 273, Issue 2, pp 325–332 | Cite as

Expression of immediate-early genes in the dorsal cochlear nucleus in salicylate-induced tinnitus

  • Shou-Sen Hu
  • Ling Mei
  • Jian-Yong Chen
  • Zhi-Wu HuangEmail author
  • Hao Wu
Otology

Abstract

Spontaneous neuronal activity in dorsal cochlear nucleus (DCN) may be involved in the physiological processes underlying salicylate-induced tinnitus. As a neuronal activity marker, immediate-early gene (IEG) expression, especially activity-dependent cytoskeletal protein (Arc/Arg3.1) and the early growth response gene-1 (Egr-1), appears to be highly correlated with sensory-evoked neuronal activity. However, their relationships with tinnitus induced by salicylate have rarely been reported in the DCN. In this study, we assessed the effect of acute and chronic salicylate treatment on the expression of N-methyl D-aspartate receptor subunit 2B (NR2B), Arg3.1, and Egr-1. We also observed ultrastructural alterations in the DCN synapses in an animal model of tinnitus. Levels of mRNA and protein expression of NR2B and Arg3.1 were increased in rats that were chronically administered salicylate (200 mg/kg, twice daily for 3, 7, or 14 days). These levels returned to baseline 14 days after cessation of treatment. However, no significant changes were observed in Egr-1 gene expression in any groups. Furthermore, rats subjected to long-term salicylate administration showed more presynaptic vesicles, thicker and longer postsynaptic densities, and increased synaptic interface curvature. Alterations of Arg3.1 and NR2B may be responsible for the changes in the synaptic ultrastructure. These changes confirm that salicylate can cause neural plasticity changes at the DCN level.

Keywords

Tinnitus Arg3.1 Egr-1 NR2B Dorsal cochlear nucleus 

Notes

Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant Nos 81170917 and 30973298) and by the Creative Project of the Shanghai Municipal Education Committee (Grant No. 12ZZ103) to Zhi-Wu Huang.

Conflict of interest

The authors do not have any possible conflicts of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Shou-Sen Hu
    • 1
    • 2
  • Ling Mei
    • 1
    • 2
  • Jian-Yong Chen
    • 1
    • 2
  • Zhi-Wu Huang
    • 1
    • 2
    Email author
  • Hao Wu
    • 1
    • 2
  1. 1.Department of Otolaryngology-Head and Neck Surgery, Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghaiChina
  2. 2.Ear InstituteShanghai Jiao Tong University School of MedicineShanghaiChina

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