Advertisement

European Archives of Oto-Rhino-Laryngology

, Volume 272, Issue 10, pp 2783–2789 | Cite as

The lack of 4-hydroxynonenal in otosclerotic bone tissue in Ethiopian population

  • Milan RudicEmail author
  • Richard Wagner
  • Eric Willkinson
  • Giovanni Danese
  • Nega Kiros
  • Kamelija Zarkovic
  • Neven Zarkovic
Otology

Abstract

In Ethiopians, like in other Africans, the incidence of otosclerosis is lower than in Western and Asian populations. Unfortunately, due to the lack of available otorhinolaryngology specialists many patients are not treated and suffer the progression of the disease and severe hearing loss. This program of the Global ENT Outreach Organization (GEO) together with the Ethiopian partners was done to help some of these patients and in parallel to evaluate the presence of the oxidative stress bioactive marker 4-hydroxynonenal (HNE), which is known as major lipid peroxidation product and the second messenger of free radicals, in the otosclerotic bone specimens. Namely, we described recently that as HNE acts as a bone growth regulator associated with pathogenesis of otosclerosis. The prospective study conducted at the ENT Department of the Migbare Senay General Hospital, Addis Ababa, Ethiopia in June 2012, under the auspices of the Global ENT Outreach Organization, USA. Altogether 36 patients (male = 12, female = 24) underwent surgery due to the previous otosclerosis diagnosis based on the clinical and audiometric findings. The bone samples were harvested from patients with intraoperatively confirmed otosclerosis diagnosis. Immunohistochemistry for HNE-modified proteins was carried out on formalin-fixed paraffin-embedded specimens. The presence of HNE was found in almost all bone samples analyzed, without particular difference in the HNE distribution pattern between the otosclerotic and respective control bone specimens. Although there was no significant association between the HNE appearance and otosclerotic bone outgrowth observed, several cases have shown tendency of higher HNE expression in patients with more severe hearing loss. The results of the present study are in contrast with our previous findings obtained on European patients most likely due to the differences between studied population groups.

Keywords

Oxidative stress Otosclerosis Pathophysiology 

Notes

Acknowledgments

Migbare Senay General Hospital staff (ENT surgery department and operating theater staff) The Department of Pathology and Forensic Medicine staff, General Hospital Zadar, Croatia and EU grants COST Action CM1001.

References

  1. 1.
    Donaldson JA, Snyder M (1993) Otosclerosis. In: Cummings CW, Frederickson JM, Harker LA, Krause CJ, Schuller DE (eds) Otolaryngology-head and neck surgery. Mosby-Year Book, St Louis, pp 2997–3016Google Scholar
  2. 2.
    Liktor B, Csomor P, Karosi T (2013) Detection of otosclerosis-specific Measles Virus receptor (Cd46) protein isoforms. ISRN OtolaryngolGoogle Scholar
  3. 3.
    Declau F, van Spaendonck M, Timmermans JP, Michaels L, Liang J, Qiu JP, van de Heyning P (2007) Prevalence of histologic otosclerosis: an unbiased temporal bone study in Caucasians. Adv Otorhinolaryngol 67:6–16Google Scholar
  4. 4.
    Chole RA, McKenna M (2001) Pathophysiology of otosclerosis. Otol Neurotol 22:49–257Google Scholar
  5. 5.
    Grayeli AB, Sterkers O, Roulleau P, Elbaz P, Ferrary E, Silve C (1999) Parathyroid hormone-parathyroid hormone related peptide receptor expression and function in otosclerosis. Am J Physiol 277:E10005–E11012Google Scholar
  6. 6.
    Grayeli AB, Palmer P, Tran Ba Huy P, Soudant J, Sterkers O, Lebon P, Ferrary E (2000) No evidence of measles virus in stapes samples from patients with otosclerosis. J Clin Microbiol 38:2655–2660PubMedCentralPubMedGoogle Scholar
  7. 7.
    Menger DJ, Tange RA (2003) The etiology of otosclerosis: a review of the literature. Clin Otolaryngol 28:112–120CrossRefPubMedGoogle Scholar
  8. 8.
    Thys M, Van Camp G (2009) Genetics of otosclerosis. Otol Neurotol 30:101–1032CrossRefGoogle Scholar
  9. 9.
    Topsakal V, Fransen E, Schmerber S, Declau F, Yung M, Gordts F, Van Camp G, Van de Heyning P (2006) Audiometrics analyses confirm a cochlear component, disproportional to age, in stapedial otosclerosis. Otol Neurotol 27:781–787CrossRefPubMedGoogle Scholar
  10. 10.
    Imauchi Y, Lombes M, Laine P, Sterkers O, ferrary E, Grayeli AB (2006) Glucocorticoids inhibit diastrophic dysplasia sulfate transporter activity in otosclerosis by interleukin-6. Laryngoscope 116:1647–1650CrossRefPubMedGoogle Scholar
  11. 11.
    Imauchi Y, Jeunemaitre X, Boussion M, Ferrary E, Sterkers O, Bozorg Grayeli A (2008) Relation between renin-angiotensin-aldosterone system and otosclerosis: a genetic association and in vitro study. Otol Neurotol 29:295–301CrossRefPubMedGoogle Scholar
  12. 12.
    Rudić M, Nguyen C, Nguyen Y, Milković L, Zarković N, Sterkers O, Ferrary E, Bozorg Grayeli A (2012) Effects of angiotensin II on inflammation pathways in human primary bone cell cultures in otosclerosis. Audiol Neurootol 17:169–178CrossRefPubMedGoogle Scholar
  13. 13.
    Lee SH, Goto T, Oe T (2008) A novel 4-oxo-2(E)-nonenal-derived modification to angiotensin II: oxidative decarboxylation of N-terminal aspartic acid. Chem Res Toxicol 21:2237–2244CrossRefPubMedGoogle Scholar
  14. 14.
    Griendling KK, Ushio-Fukai M (2000) Reactive oxygen species as mediators of angiotensin II signaling. Regul Pept 91:21–27CrossRefPubMedGoogle Scholar
  15. 15.
    Rudić M, Milković L, Žarković K, Borović-Šunjić S, Sterkers O, Waeg G, Ferrary E, Bozorg Grayeli A, Žarković N (2013) The effects of angiotensin II and the oxidative stress mediator 4-hydroxynonenal on human osteoblast-like cell growth: possible relevance to otosclerosis. Free Radic Biol Med 57:22–28CrossRefPubMedGoogle Scholar
  16. 16.
    Ciorba A, Gasparini P, Chicca M, Pinamonti S, Martini A (2010) Reactive oxygen species in human inner ear perylimph. Acta Otolaryngol 130:240–246CrossRefPubMedGoogle Scholar
  17. 17.
    Corbacella E, Lanzoni I, Ding D, Previati M, Salvi R (2004) Minocycline attenuates gentamicin in induced hair cell loss in neonatal cochlear culture. Hear Res 197:11–18CrossRefPubMedGoogle Scholar
  18. 18.
    Wang J, Dib M, Lenoir M, Vago P, Eybalin M, Hameg A (2002) Riluzole rescues cochlear sensory cells from acoustic trauma in guinea pig. Neuroscience 111:635–648CrossRefPubMedGoogle Scholar
  19. 19.
    Henderson D, Bielefeld EC, Harris KC, Hu BH (2006) The role of oxidative stress in noise- induced hearing loss. Ear Hear 27:1–19CrossRefPubMedGoogle Scholar
  20. 20.
    Zarkovic N (2003) 4-Hydroxynonenal as a bioactive marker of pathophysiological processes. Mol Aspects Med 24:281–291CrossRefPubMedGoogle Scholar
  21. 21.
    Borovic Sunjic S, Cipak A, Rabuzin F, Wildburger R, Zarkovic N (2005) The influence of 4-hydroxy-2-nonenal on proliferation, differentiation and apoptosis of human osteosarcoma cells. BioFactors 24:141–148CrossRefGoogle Scholar
  22. 22.
    Borovic S, Cipak A, Mennitzer A, Kejla Z, Perovic D, Waeg G, Zarkovic N (2007) Differential sensitivity to 4-hydroxynonenal of normal and malignant mesenchymal cells. Redox Rep 12:50–54CrossRefPubMedGoogle Scholar
  23. 23.
    Mrakovcic L, Wildburger R, Jaganjac M, Cindric M, Cipak A, Borovic-Sunjic S, Waeg G, Milankovic AM, Zarkovic N (2010) Lipid peroxidation product 4-hydroxynonenal as factor of oxidative homeostasis supporting bone regeneration with bioactive glasses. Acta Biochim Pol 57:173–178PubMedGoogle Scholar
  24. 24.
    Tomek MS, Brown MR, Mani SR, Ramesh A, Srisailapathy CR, Coucke P, Zbar RI, Bell AM, McGuirt WT, Fukushima K, Willems PJ, Van Camp G, Smith RJ (1998) Localization of a gene for otosclerosis to chromosome 15q25-q26. Hum Mol Genet 7:285–290CrossRefPubMedGoogle Scholar
  25. 25.
    Schrauwen I, Ealy M, Fransen E, Vanderstraeten K, Thys M, Meyer NC, Cosgaera M, Huber A, Mayzzoli M, Pfister M, Smith RJ, Van Camp G (2010) Genetic variants in the RELN gene are associated with otosclerosis in multiple European populations. Hum Genet 127:155–162CrossRefPubMedGoogle Scholar
  26. 26.
    Thys M, Schrauwen I, Vanderstraeten K, Dieltjens N, Fransen E, Ealy M, Cremers CWRJ, van de Heyning P, Vincent R, Offeciers E, Smith RH, van Camp G (2009) Detection of Rare Nonsynonymous Variants in TGFB1 in Otosclerosis Patients. Ann Hum Genet 73:171–175CrossRefPubMedGoogle Scholar
  27. 27.
    Thys M, Schrauwen I, Vanderstraeten K, Janssens K, Dieltjens N, Van Den Bogaert Kris, Fransen E, Chen W, Ealy M, Claustres M, Cremers CRWJ, Dhooge I, Declau F, Claes J, Van de Heyning P, Vincent R, Somers T, Offeciers E, Smith RJH, Van Camp G (2007) The coding polymorphism T263I in TGF-b1 is associated with otosclerosis in two independent populations. Hum Mol Genet 16:2021–2030CrossRefPubMedGoogle Scholar
  28. 28.
    Tshifularo M, Joseph CA (2008) Otosclerosis and TGF-β1 gene in black South Africans. South African Med J 98:720–723Google Scholar
  29. 29.
    Bodo M, Venti G, Baroni T, Bellucci C, Giammarioli M, Donti E, Paludetti G, Stabellini G, Carinci P (1995) Phenotype of in vitro human otosclerotic cells and its modulation by TGF beta. Cel Mol Biol 1995(41):1039–1049Google Scholar
  30. 30.
    Csomor P, Liktor B, Szekanecz Z, Sziklai I, Karosi T (2012) Expression of bone morphogenetic protein 2, 4, 5, and 7 correlates with histological activity of otosclerotic foci. Acta Oto-Laryngol 132:624–631CrossRefGoogle Scholar
  31. 31.
    Biasi F, Vizio B, Mascia C, Gaia E, Zarkovic N, Chiarpotto E, Leonarduzzi G, Poli G (2006) JNK up-regulation as a key event in the pro-apoptotic interaction between TGF-ß1 and 4-hydroxynonenal in colon mucosa. Free Radic Biol Med 40:443–454CrossRefGoogle Scholar
  32. 32.
    Negre-Salvayre A, Auge N, Ayala V, Basaga H, Boada J, Brenke R, Chapple S, Cohen G, Feher J, Grune T, Lengyel G, Mann GE, Pamplona R, Poli G, Manuel Otin P, Riahi Y, Salvayre R, Sasson S, Serrano J, Shamni O, Siems W, Richard RCM, Wiswedel I, Zarkovic K, Zarkovic N (2010) Pathological aspects of lipid peroxidation. Free Radic Res 44:1125–1171CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Milan Rudic
    • 1
    Email author
  • Richard Wagner
    • 2
  • Eric Willkinson
    • 2
    • 3
  • Giovanni Danese
    • 2
    • 4
  • Nega Kiros
    • 5
  • Kamelija Zarkovic
    • 6
  • Neven Zarkovic
    • 7
  1. 1.ENT DepartmentGeneral Hospital ZadarZadarCroatia
  2. 2.Global ENT Outreach Organization (GEO)CoupevilleUSA
  3. 3.House Ear ClinicLos AngelesUSA
  4. 4.Bergamo University HospitalBergamoItaly
  5. 5.Migbare Senay General HospitalAddis AbabaEthiopia
  6. 6.Division of PathologyClinical Hospital Centre Zagreb, University Medical SchoolZagrebCroatia
  7. 7.Institute Ruđer BoškovićZagrebCroatia

Personalised recommendations