European Archives of Oto-Rhino-Laryngology

, Volume 271, Issue 12, pp 3333–3340 | Cite as

Localization of prestin and expression in the early period after radiation in mice

  • Chen Yang
  • Wei Zhang
  • Xiao-Long Liu
  • Yong Liang
  • Ya-Wei Yuan
  • Chen Ren
  • Jin-Hao Peng
Miscellaneous
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Abstract

This study aimed to examine expression and microstructural distribution of prestin in outer hair cells, and the effect of dose and time of radiation on prestin expression in the BALB/c mouse. We also investigated the molecular biological characteristics of prestin and possible mechanisms of sensorineural hearing loss caused by radiation. Seventy 4-week-old mice were randomly divided into four groups, including one control group and three experimental groups. Each experimental group was randomly divided into two groups, which were killed to collect specimens of the cochlea on the 3rd and 7th days after exposure to different doses of 8, 12, and 16 Gy radiation. These cochleas were embedded in paraffin, and then cut into sections. The sections were immunostained with anti-prestin antibodies. The distribution of prestin was observed under optical microscopy and the density of prestin-positive expression was quantitatively calculated by Image-Pro Plus. Prestin had high expression in the lateral membrane and low expression in the cytoplasm of outer hair cells above the nucleus. The density of prestin protein expression of the basal turn was not significantly different after exposure to the different doses of radiation compared with the control group, but up-regulation occurred after radiation in the apex turn. We conclude that prestin protein is mainly expressed in the lateral membrane above the nucleus. Prestin protein may be responsible for the mechanism of injury to the inner ear caused by radiation.

Keywords

Prestin protein Radiation BALB/c mouse Sensorineural hearing loss 
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Notes

Acknowledgments

This study was supported by a Grant from Foreign Cooperation Projects of Science and Technology of Kwangtung Province.

Conflict of interest

None of the authors has any conflict of interest.

References

  1. 1.
    Winther FO (1969) Early degenerative changes in the inner ear sensory cells of the guinea pig following local X-ray irradiation. A preliminary report. Acta Otolaryngol 67:262–268PubMedCrossRefGoogle Scholar
  2. 2.
    Akmansu H, Eryilmaz A, Korkmaz H et al (2004) Ultrastructural and electrophysiologic changes of rat cochlea after irradiation. Laryngoscope 114:1276–1280PubMedCrossRefGoogle Scholar
  3. 3.
    Kim CS, Shin SO (1994) Ultrastructural changes in the cochlea of the guinea pig after fast neutron irradiation. Otolaryngol Head Neck Surg 110:419–427PubMedGoogle Scholar
  4. 4.
    Petsuksiri J, Sermsree A, Thephamongkhol K et al (2011) Sensorineural hearing loss after concurrent chemoradiotherapy in nasopharyngeal cancer patients. Radiat Oncol 6:19PubMedCentralPubMedCrossRefGoogle Scholar
  5. 5.
    Raaijmakers E, Engelen AM (2002) Is sensorineural hearing loss a possible side effect of nasopharyngeal and parotid irradiation? A systematic review of the literature. Radiother Oncol 65:1–7PubMedCrossRefGoogle Scholar
  6. 6.
    Schultz C, Goffi-Gomez MV, Pecora LP et al (2010) Hearing loss and complaint in patients with head and neck cancer treated with radiotherapy. Arch Otolaryngol Head Neck Surg 136:1065–1069PubMedCrossRefGoogle Scholar
  7. 7.
    Wang LF, Kuo WR, Ho KY et al (2004) A long-term study on hearing status in patients with nasopharyngeal carcinoma after radiotherapy. Otol Neurotol 25:168–173PubMedCrossRefGoogle Scholar
  8. 8.
    Low WK, Burgess R, Fong KW et al (2005) Effect of radiotherapy on retro-cochlear auditory pathways. Laryngoscope 115:1823–1826PubMedCrossRefGoogle Scholar
  9. 9.
    Anteunis LJ, Wanders SL, Hendriks JJ et al (1994) A prospective longitudinal study on radiation-induced hearing loss. Am J Surg 168:408–411PubMedCrossRefGoogle Scholar
  10. 10.
    Low WK, Tan MG, Chua AW et al (2009) 12th Yahya Cohen Memorial Lecture: the cellular and molecular basis of radiation-induced sensori-neural hearing loss. Ann Acad Med Singap 38:91–94PubMedGoogle Scholar
  11. 11.
    Yang XM, Lu YD, Chen Z et al (1997) An experimental study of effects of the ionizing radiation on morphology and function of the inner ear. Chin J Otorhinolaryngol Skull Base Surg 3:20–24Google Scholar
  12. 12.
    Low WK, Sun L, Tan MG et al (2008) l-N-Acetylcysteine protects against radiation-induced apoptosis in a cochlear cell line. Acta Otolaryngol 128:440–445PubMedCrossRefGoogle Scholar
  13. 13.
    Du SS, Ren C, Tian YH et al (2011) Effect of ionizing radiation on the Prestin expression in the rat cochlear outer hair cells. J Trop Med 11:1127–1130Google Scholar
  14. 14.
    Zheng J, Shen W, He DZ et al (2000) Prestin is the motor protein of cochlear outer hair cells. Nature 405:149–155PubMedCrossRefGoogle Scholar
  15. 15.
    Liberman MC, Gao J, He DZ et al (2002) Prestin is required for electromotility of the outer hair cell and for the cochlear amplifier. Nature 19:300–304CrossRefGoogle Scholar
  16. 16.
    Mazurek B, Haupt H, Amarjargal N et al (2007) Up-regulation of prestin mRNA expression in the organs of Corti of guinea pigs and rats following unilateral impulse noise exposure. Hear Res 231:73–83PubMedCrossRefGoogle Scholar
  17. 17.
    Rossiter SJ, Zhang S, Liu Y (2011) Prestin and high frequency hearing in mammals. Commun Integr Biol 4:236–239PubMedCentralPubMedCrossRefGoogle Scholar
  18. 18.
    Zheng J, Long KB, Shen W et al (2001) Prestin topology: localization of protein epitopes in relation to the plasma membrane. NeuroReport 12:1929–1935PubMedCrossRefGoogle Scholar
  19. 19.
    Belyantseva IA, Adler HJ, Curi R et al (2000) Expression and localization of prestin and the sugar transporter GLUT-5 during development of electromotility in cochlear outer hair cells. J Neurosci 20:RC116PubMedGoogle Scholar
  20. 20.
    Adler HJ, Belyantseva IA, Merritt RJ et al (2003) Expression of prestin, a membrane motor protein, in the mammalian auditory and vestibular periphery. Hear Res 184:27–40PubMedCrossRefGoogle Scholar
  21. 21.
    Huang G, Santos-Sacchi J (1993) Mapping the distribution of the outer hair cell motility voltage sensor by electrical amputation. Biophys J 65:2228–2236PubMedCentralPubMedCrossRefGoogle Scholar
  22. 22.
    Yu N, Zhu ML, Zhao HB (2006) Prestin is expressed on the whole outer hair cell basolateral surface. Brain Res 1095:51–58PubMedCentralPubMedCrossRefGoogle Scholar
  23. 23.
    Zhang B, Yin SH (2012) Experimental study the prestin distribution at the membrane of single outer hair cells of cochlea in newborn rat. Chongqing Med 41:3178–3179Google Scholar
  24. 24.
    Takahashi M, Kimura Y, Sawabe M et al (2010) Modified paraffin-embedding method for the human cochlea that reveals a fine morphology and excellent immunostaining results. Acta Otolaryngol 130:788–792PubMedCrossRefGoogle Scholar
  25. 25.
    Mahendrasingam S, Beurg M, Fettiplace R et al (2010) The ultrastructural distribution of prestin in outer hair cells: a post-embedding immunogold investigation of low-frequency and high-frequency regions of the rat cochlea. Eur J Neurosci 31:1595–1605PubMedCentralPubMedCrossRefGoogle Scholar
  26. 26.
    Chen GD (2006) Prestin gene expression in the rat cochlea following intense noise exposure. Hear Res 222:54–61PubMedCrossRefGoogle Scholar
  27. 27.
    Mu MY, Chardin S, Avan P et al (1997) Ontogenesis of rat cochlea. A quantitative study of the organ of Corti. Brain Res Dev Brain Res 99:29–37PubMedCrossRefGoogle Scholar
  28. 28.
    Zajic G, Schacht J (1987) Comparison of isolated outer hair cells from five mammalian species. Hear Res 26:249–256PubMedCrossRefGoogle Scholar
  29. 29.
    Zimmermann U, Fermin C (1996) Shape deformation of the organ of Corti associated with length changes of outer hair cell. Acta Otolaryngol 116:395–400PubMedCrossRefGoogle Scholar
  30. 30.
    Gross J, Machulik A, Amarjargal N et al (2005) Expression of prestin mRNA in the organotypic culture of rat cochlea. Hear Res 204:183–190PubMedCrossRefGoogle Scholar
  31. 31.
    Judice TN, Nelson NC, Beisel CL et al (2002) Cochlear whole mount in situ hybridization: identification of longitudinal and radial gradients. Brain Res Brain Res Protoc 9:65–76PubMedCrossRefGoogle Scholar
  32. 32.
    Liu CC, Gao SS, Yuan T et al (2011) Biophysical mechanisms underlying outer hair cell loss associated with a shortened tectorial membrane. J Assoc Res Otolaryngol 12:577–594PubMedCentralPubMedCrossRefGoogle Scholar
  33. 33.
    Verpy E, Weil D, Leibovici M et al (2008) Stereocilin-deficient mice reveal the origin of cochlear waveform distortions. Nature 456:255–258PubMedCentralPubMedCrossRefGoogle Scholar
  34. 34.
    Minekawa A, Abe T, Inoshita A et al (2009) Cochlear outer hair cells in a dominant-negative connexin 26 mutant mouse preserve non-linear capacitance in spite of impaired distortion product otoacoustic emission. Neuroscience 164:1312–1319PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Chen Yang
    • 1
  • Wei Zhang
    • 1
  • Xiao-Long Liu
    • 1
  • Yong Liang
    • 1
  • Ya-Wei Yuan
    • 2
  • Chen Ren
    • 2
  • Jin-Hao Peng
    • 2
  1. 1.Department of Otorhinolaryngology-Head and Neck Surgery, Nan Fang HospitalSouthern Medical UniversityGuangzhouChina
  2. 2.Department of Radiotherapy, Nan Fang HospitalSouthern Medical UniversityGuangzhouChina

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