European Archives of Oto-Rhino-Laryngology

, Volume 271, Issue 5, pp 1107–1112 | Cite as

Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats

  • Markus GugatschkaEmail author
  • Herwig Ainödhofer
  • Hans-Jürgen Gruber
  • Matthias Graupp
  • Petra Kieslinger
  • Karl Kiesler
  • Amulya Saxena
  • Shigeru Hirano
  • Gerhard Friedrich


Vocal fold (VF) fibroblasts are the central subject of interest in fibrogenesis and wound healing after VF injury. Scar fibroblasts (SF) exhibit an aberrant production of several extracellular matrix (ECM) components which lead either to VF fibrosis or scarless wound healing. This study aimed to investigate the role of age at the time of injury on ECM production of SF. This is designed as an animal study. VF injury was established unilaterally in eight male Sprague-Dawley rats [3 months of age (n = 4), 11 months of age (n = 4)], while the other side was left intact. Three months after injury the larynges were excised and fibroblasts were extracted from VF [normal fibroblasts (NF)–scar fibroblasts (SF)] and cultured in vitro. After first passage, VF fibroblasts were plated in 24-well plates and levels of hyaluronic acid (HA) and collagen type I were determined enzymatically from supernatant after 24 and 72 h. Cultured SF from younger animals produced significantly higher levels of HA compared to NF fibroblasts from the same animals. HA concentrations of the older animals did not differ significantly between the NF and SF cultures, but the range in SF cultures was large. In contrast to previous studies, we found that even 3 months after VF injury cultured SF from young animals expressed higher levels of HA in comparison to SF from older animals. No difference in collagen levels were observed between the younger and older animals. Age of animals is an essential factor during VF healing and has to be considered for study design.


Vocal fold scar Scar fibroblasts Rat vocal fold scar model 



The authors would like to thank Richard Ackbar, PhD for discussing and proofreading. This project was supported by the Styrian government.

Conflict of interest



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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Markus Gugatschka
    • 1
    Email author
  • Herwig Ainödhofer
    • 2
  • Hans-Jürgen Gruber
    • 3
  • Matthias Graupp
    • 1
  • Petra Kieslinger
    • 3
  • Karl Kiesler
    • 1
  • Amulya Saxena
    • 2
  • Shigeru Hirano
    • 4
  • Gerhard Friedrich
    • 1
  1. 1.Department of PhoniatricsENT University Hospital Graz, Medical University GrazGrazAustria
  2. 2.Department of Pediatric- and Adolescent SurgeryMedical University GrazGrazAustria
  3. 3.Clinical Institute of Medical and Chemical Laboratory DiagnosticsMedical University GrazGrazAustria
  4. 4.Department of Otolaryngology HNS, Graduate School of MedicineKyoto UniversityKyotoJapan

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