European Archives of Oto-Rhino-Laryngology

, Volume 270, Issue 5, pp 1695–1700 | Cite as

Modulation of wireless (2.45 GHz)-induced oxidative toxicity in laryngotracheal mucosa of rat by melatonin

  • Giray Aynali
  • Mustafa Nazıroğlu
  • Ömer Çelik
  • Mustafa Doğan
  • Murat Yarıktaş
  • Hasan Yasan
Laryngology

Abstract

It is well known that oxidative stress induces larynx cancer, although antioxidants induce modulator role on etiology of the cancer. It is well known that electromagnetic radiation (EMR) induces oxidative stress in different cell systems. The aim of this study was to investigate the possible protective role of melatonin on oxidative stress induced by Wi-Fi (2.45 GHz) EMR in laryngotracheal mucosa of rat. For this purpose, 32 male rats were equally categorized into four groups, namely controls, sham controls, EMR-exposed rats, EMR-exposed rats treated with melatonin at a dose of 10 mg/kg/day. Except for the controls and sham controls, the animals were exposed to 2.45 GHz radiation during 60 min/day for 28 days. The lipid peroxidation levels were significantly (p < 0.05) higher in the radiation-exposed groups than in the control and sham control groups. The lipid peroxidation level in the irradiated animals treated with melatonin was significantly (p < 0.01) lower than in those that were only exposed to Wi-Fi radiation. The activity of glutathione peroxidase was lower in the irradiated-only group relative to control and sham control groups but its activity was significantly (p < 0.05) increased in the groups treated with melatonin. The reduced glutathione levels in the mucosa of rat did not change in the four groups. There is an apparent protective effect of melatonin on the Wi-Fi-induced oxidative stress in the laryngotracheal mucosa of rats by inhibition of free radical formation and support of the glutathione peroxidase antioxidant system.

Keywords

Melatonin Larynx Trachea Oxidative stress Wireless devices 

References

  1. 1.
    Öngel K, Gümral N, Özgüner F (2010) The potential effects of electromagnetic field: a review. Cell Membr Free Radic Res 1:85–89Google Scholar
  2. 2.
    Crouzier D, Testylier G, Perrin A et al (2007) Which neurophysiologic effects at low level 2.45 GHz RF exposure? Pathol Biol (Paris) 55:235–241CrossRefGoogle Scholar
  3. 3.
    Lukac N, Massanyi P, Roychoudhury S et al (2011) In vitro effects of radiofrequency electromagnetic waves on bovine spermatozoa motility. J Environ Sci Health A Tox Hazard Subst Environ Eng 46:1417–1423. doi:10.1080/10934529.2011.607037 PubMedGoogle Scholar
  4. 4.
    Gümral N, Naziroglu M, Koyu A et al (2009) Effects of selenium and L-Carnitine on oxidative stress in blood of rat induced by 2.45-GHz radiation from wireless devices. Biol Trace Elem Res 132:153–163. doi:10.1007/s12011-009-8372-3 PubMedCrossRefGoogle Scholar
  5. 5.
    Türker Y, Nazıroğlu M, Gümral N et al (2011) Selenium and L-carnitine reduce oxidative stress in the heart of rat induced by 2.45-GHz radiation from wireless devices. Biol Trace Elem Res 143:1640–1650. doi:10.1007/s12011-011-8994-0 PubMedCrossRefGoogle Scholar
  6. 6.
    Anderson ME (1998) Glutathione: an overview of biosynthesis and modulation. Chem Biol Interac. 111–112:1–14CrossRefGoogle Scholar
  7. 7.
    Nazıroğlu M (2009) Role of selenium on calcium signaling and oxidative stress- induced molecular pathways in epilepsy. Neurochem Res 34:2181–2191. doi:10.1007/s11064-009-0015-8 PubMedCrossRefGoogle Scholar
  8. 8.
    Nazıroğlu M, Ciğ B, Doğan S et al (2012) 2.45-Gz wireless devices induce oxidative stress and proliferation through cytosolic Ca²+ influx in human leukemia cancer cells. Int J Radiat Biol 88:449–456. doi:10.3109/09553002.2012.682192 PubMedCrossRefGoogle Scholar
  9. 9.
    Tan DX, Manchester LC, Terron MP et al (2007) One molecule, many derivatives: a never-ending interaction of melatonin with reactive oxygen and nitrogen species? J Pineal Res 42:28–42. doi:10.1111/j.1600-079X.2006.00407.x PubMedCrossRefGoogle Scholar
  10. 10.
    Fic M, Podhorska-Okolow M, Dziegiel P et al (2007) Effect of melatonin on cytotoxicity of doxorubicin toward selected cell lines (human keratinocytes, lung cancer cell line A-549, laryngeal cancer cell line Hep-2). In Vivo. 21:513–518PubMedGoogle Scholar
  11. 11.
    Nazıroğlu M, Çelik Ö, Özgül C et al (2012) Melatonin modulates wireless (2.45 GHz)-induced oxidative injury through TRPM2 and voltage gated Ca(2+) channels in brain and dorsal root ganglion in rat. Physiol Behav 105:683–692. doi:10.1016/j.physbeh.2011.10.005 PubMedCrossRefGoogle Scholar
  12. 12.
    Karaman E, Uzun H, Papila I et al (2010) Serum paraoxonase activity and oxidative DNA damage in patients with laryngeal squamous cell carcinoma. J Craniofac Surg. 21:1745–1749. doi:10.1097/SCS.0b013e3181f4040a PubMedCrossRefGoogle Scholar
  13. 13.
    Manjunath MK, Annam V, Suresh DR (2010) Significance of free radical injury in laryngeal and hypopharyngeal cancers. J Laryngol Otol 124:315–317. doi:10.1017/S0022215109991721 PubMedCrossRefGoogle Scholar
  14. 14.
    Placer ZA, Cushman L, Johnson BC (1966) Estimation of products of lipid peroxidation (malonyldialdehyde) in biological fluids. Anal Biochem 16:359–364PubMedCrossRefGoogle Scholar
  15. 15.
    Sedlak J, Lindsay RHC (1968) Estimation of total, protein bound and non-protein sulfhydryl groups in tissue with Ellmann’s reagent. Anal Biochem 25:192–205PubMedCrossRefGoogle Scholar
  16. 16.
    Lawrence RA, Burk RF (1976) Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71:952–958PubMedCrossRefGoogle Scholar
  17. 17.
    Lowry OH, Rosebrough NJ, Farr AL et al (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  18. 18.
    Kovacic P, Somanathan R (2008) Unifying mechanism for eye toxicity: electron transfer, reactive oxygen species, antioxidant benefits, cell signaling and cell Membranes. Cell Membr Free Radic Res 2:56–69. doi:10.3109/10799890903582578 Google Scholar
  19. 19.
    Kumar S, Kesari KK, Behari J (2011) The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave field. Clinics (Sao Paulo) 66:1237–1245CrossRefGoogle Scholar
  20. 20.
    Taysi S, Uslu C, Akcay F et al (2003) Malondialdehyde and nitric oxide levels in the plasma of patients with advanced laryngeal cancer. Surg Today 33:651–654PubMedCrossRefGoogle Scholar
  21. 21.
    Kacakci A, Aslan I, Toplan S et al (2009) Significance of the counteracting oxidative and antioxidative systems in the pathogenesis of laryngeal carcinoma. J Otolaryngol Head Neck Surg. 38:172–177PubMedGoogle Scholar
  22. 22.
    Inci E, Civelek S, Seven A et al (2003) Laryngeal cancer: in relation to oxidative stress. Tohoku J Exp Med 200:17–23PubMedCrossRefGoogle Scholar
  23. 23.
    Ceyhan AM, Akkaya VB, Güleçol SC et al (2012) Protective effects of β-glucan against oxidative injury induced by 2.45-GHz electromagnetic radiation in the skin tissue of rats. Arch Dermatol Res 304:521–527. doi:10.1007/s00403-012-1205-9 PubMedCrossRefGoogle Scholar
  24. 24.
    Naziroğlu M, Gümral N (2009) Modulator effects of L-carnitine and selenium on wireless devices (2.45 GHz)-induced oxidative stress and electroencephalography records in brain of rat. Int J Radiat Biol 85:680–689. doi:10.1080/09553000903009530 PubMedCrossRefGoogle Scholar
  25. 25.
    Kesari KK, Kumar S, Behari J (2011) 900-MHz microwave radiation promotes oxidation in rat brain. Electromagn Biol Med 30:219–234. doi:10.3109/15368378.2011.587930 PubMedCrossRefGoogle Scholar
  26. 26.
    Reiter RJ, Tan DX, Osuna C et al (2000) Actions of melatonin in the reduction of oxidative status. J Biomed Sci 7:444–458PubMedCrossRefGoogle Scholar
  27. 27.
    Köylü H, Mollaoglu H, Ozguner F et al (2006) Melatonin modulates 900 MHz microwave-induced lipid peroxidation changes in rat brain. Toxicol Ind Health 22:211–216PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Giray Aynali
    • 1
    • 4
  • Mustafa Nazıroğlu
    • 2
  • Ömer Çelik
    • 2
  • Mustafa Doğan
    • 3
  • Murat Yarıktaş
    • 1
  • Hasan Yasan
    • 1
  1. 1.Ear, Nose and Throat, Head and Neck Surgery DepartmentSchool of Medicine, Suleyman Demirel UniversityIspartaTurkey
  2. 2.Biophysics DepartmentSchool of Medicine, Suleyman Demirel UniversityIspartaTurkey
  3. 3.Ear, Nose and Throat, Head and Neck Surgery DepartmentIsparta State HospitalIspartaTurkey
  4. 4.IspartaTurkey

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