European Archives of Oto-Rhino-Laryngology

, Volume 267, Issue 3, pp 391–395 | Cite as

Identification of polymorphisms in the RNase3 gene and the association with allergic rhinitis

  • Inhong Kang
  • Xue-hua An
  • Yeon-Kyun Oh
  • Sang Heon Lee
  • Ha Min Jung
  • Soo-Cheon Chae
  • Jae Hoon Lee
Rhinology

Abstract

Eosinophil cationic protein (ECP), a potent cytotoxic molecule, is released by activated eosinophils. ECP has been suggested to be involved in tissue remodeling of allergic diseases. The ECP (RNase3) gene is a candidate gene in atopic diseases. RNase3 polymorphisms have been reported to have an association with atopy. We determined whether polymorphisms in the RNase3 gene are associated with allergic rhinitis in a Korean population. The Taqman assay, restriction fragment length polymorphism (PCR-RFLP), and high-resolution melt (HRM) were used for genotyping. Three single nucleotide polymorphisms (SNPs; g.−550A>G, g.371G>C, and g.499G>C) were identified. The genotype of the SNPs was analyzed in patients with allergic rhinitis and controls without allergic rhinitis. The genotype and allele frequencies were compared between both groups. The genotype frequencies of the g.−550A>G and g.371G>C SNPs were not significantly different between patients with allergic rhinitis and controls (P > 0.05). However, in patients with allergic rhinitis, the genotype and allele frequencies of the g.499G>C SNP of RNase 3 were significantly different from those of the control group (P < 001, P = 0.034, respectively). Haplotype analysis demonstrated the presence of the following five different (−550)-(+371)-(+499) major haplotypes: A-G-G, G-C-C, G-G-G, G-C-G, and A-G-C. The G-C-G haplotype was positively associated with allergic rhinitis (P = 0.048), while the G-G-G haplotype was negatively associated with allergic rhinitis (P = 0.004). Our study suggests that RNase3 polymorphisms are potentially associated with susceptibility to allergic rhinitis.

Keywords

RNase3 Allergic rhinitis Polymorphism Haplotype 

References

  1. 1.
    Amin K, Ludviksdottir D, Janson C, Nettelbladt O, Bjornsson E, Roomans GM, Boman G, Seveus L, Venqe P (2000) Inflammation and structural changes in the airways of patients with atopic and nonatopic asthma. Am J Respir Crit Care Med 162:2295–2301PubMedGoogle Scholar
  2. 2.
    Jonsson UB, Bystrom J, Stalenheim G, Venge P (2002) Polymorphism of the eosinophil cationic protein-gene is related to the expression of allergic symptoms. Clin Exp Allergy 32:1092–1095CrossRefPubMedGoogle Scholar
  3. 3.
    Jonsson UB, Bystrom J, Stalenheim G, Venge P (2006) A (G→C) transversion in the 3′ UTR of the human ECP (eosinophil cationic protein) gene correlates to the cellular content of ECP. J Leukoc Biol 79:846–851CrossRefPubMedGoogle Scholar
  4. 4.
    Kim JJ, Lee JH, Jang CH, Kim YS, Chae SC, Chung HT, Choi TW, Lee JH (2004) Chemokine RANTES promoter polymorphisms in allergic rhinitis. Laryngoscope 114:666–669CrossRefPubMedGoogle Scholar
  5. 5.
    Kim JJ, Kim HJ, Lee KI, Chung HT, Lee JH (2004) Association between polymorphisms of the angiotensin-converting enzyme and angiotensinogen genes and allergic rhinitis in a Korean population. Ann Otol Rhinol Laryngol 113:297–302PubMedGoogle Scholar
  6. 6.
    Kim JJ, Min JY, Lee JH (2007) Polymorphisms in the IL-13 and IL-4 receptor alpha genes and allergic rhinitis. Eur Arch Otorhinolaryngol 264:395–399CrossRefPubMedGoogle Scholar
  7. 7.
    Martin LB, Kita H, Leiferman KM, Gleich GJ (1996) Eosinophils in allergy: role in disease, degranulation and cytokines. Int Arch Allergy Immunol 109:207–215CrossRefPubMedGoogle Scholar
  8. 8.
    Munthe-Kaas MC, Gerritsen J, Carlsen KH, Undlien D, Egeland T, Skinningsrud B, Torres T, Carlsen KL (2007) Eosinophil cationic protein (ECP) polymorphisms and association with asthma, s-ECP levels and related phenotypes. Allergy 62:429–436CrossRefPubMedGoogle Scholar
  9. 9.
    Noguchi E, Iwama A, Takeda K, Takeda T, Kamioka M, Ichikawa K, Akeba T, Arinami T, Shibasaki M (2003) The promoter polymorphism in the eosinophil cationic protein gene and its influence on the serum eosinophil cationic protein level. Am J Respir Crit Care Med 167:180–184CrossRefPubMedGoogle Scholar
  10. 10.
    Rosenberg HF, Domachowske JB (1995) Rapid evolution of a unique family of primate ribonuclease genes. Nat Genet 10:219–223CrossRefPubMedGoogle Scholar
  11. 11.
    Rosenberg HF, Domachowske JB (2001) Eosinophils, eosinophil ribonucleases, and their role in host defense against respiratory virus pathogens. J Leukoc Biol 70:691–698PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Inhong Kang
    • 1
  • Xue-hua An
    • 2
  • Yeon-Kyun Oh
    • 3
  • Sang Heon Lee
    • 4
  • Ha Min Jung
    • 4
  • Soo-Cheon Chae
    • 1
  • Jae Hoon Lee
    • 4
  1. 1.Department of Pathology, Wonkwang Institute of Medical ScienceWonkwang University School of MedicineIksanSouth Korea
  2. 2.Jeollabuk-Do Bioindustry Development Institute Innovation CenterJeonjuSouth Korea
  3. 3.Department of Pediatric, Wonkwang Institute of Medical ScienceWonkwang University School of MedicineIksanSouth Korea
  4. 4.Department of Otolaryngology, Wonkwang Institute of Medical ScienceWonkwang University School of MedicineIksanSouth Korea

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