European Archives of Oto-Rhino-Laryngology

, Volume 266, Issue 5, pp 663–667 | Cite as

The ototoxic effect of boric acid solutions applied into the middle ear of guinea pigs

  • Sedat Öztürkcan
  • Rıza Dündar
  • Hüseyin Katılmış
  • Ali Ekber İlknur
  • Sinem Aktaş
  • Senem Hacıömeroğlu


This study analyzed the ototoxic effects of boric acid solutions. Boric acid solutions have been used as otologic preparations for many years. Boric acid is commonly found in solutions prepared with alcohol or distilled water but can also be found in a powder form. These preparations are used for both their antiseptic and acidic qualities in external and middle ear infections. We investigated the ototoxic effect of boric acid solutions on guinea pigs. We are unaware of any similar, previously published study of this subject in English. The study was conducted on 28 young albino guinea pigs. Prior to application of the boric acid solution under general anesthesia, an Auditory Brainstem Response (ABRs) test was applied to the right ear of the guinea pigs. Following the test, a perforation was created on the tympanic membrane of the right ear of each guinea pig and small gelfoam pieces were inserted into the perforated area. Test solutions were administered to the middle ear for 10 days by means of a transcanal route. Fifteen days after inserting the gelfoams in all of the guinea pigs, we anasthesized the guinea pigs and removed the gelfoams from the perforated region of the ear and then performed an ABRs on each guinea pig. The ABRs were within the normal range before the applications. After the application, no significant changes were detected in the ABRs thresholds in neither the saline group nor the group administered boric acid and distilled water solution; however, significant changes were detected in the ABRs thresholds of the Gentamicine and boric acid and alcohol solution groups. We believe that a 4% boric acid solution prepared with distilled water can be a more reliable preparation than a 4% boric acid solution prepared with alcohol.


Boric acid Ototoxicity Inner ear ABR 



The authors would like to thank the audiology unit of The İzmir Atatürk Training and Research Hospital. We would also especially like to thank expert audiologist Nimet Akyıl whose knowledge and assistance substantially aided our study.

Conflict of interest statement

The authors declare that they have no conflict of interest.


  1. 1.
    Brummett RE, Morrison RB (1990) The incidence of aminoglycoside antibiotic-induced hearing loss. Arch Otolaryngol Head Neck Surg 116:406–410PubMedGoogle Scholar
  2. 2.
    Ganz H (1989) Local antibiotic therapy of bacterial ear infections. HNO 37:386–388PubMedGoogle Scholar
  3. 3.
    Thorp MA, Kruger J, Oliver S, Nilssen EL, Prescott CA (1998) The antibacterial activity of acetic acid and Burrow’s solution as topical otological preparations. J Laryngol Otol 112:925–928PubMedGoogle Scholar
  4. 4.
    Morizono T, Johnstone BM (1975) Ototoxicity of chloramphenicol ear drops with propylene glycol as solvent. Med J Aust 2:634–638PubMedGoogle Scholar
  5. 5.
    Brummett RE, Harris RF, Lindgren JA (1976) Detection of ototoxicity from drugs applied topically to the middle ear space. Laryngoscope 86:1177–1187PubMedCrossRefGoogle Scholar
  6. 6.
    Hawkins JE, Johnson LG (1981) Histopathology of cochlear and vestibular ototoxicity in laboratory animals in aminoglycoside’s ototoxicity. Little Brown and co, Boston, pp 175–197Google Scholar
  7. 7.
    Serin GM, Ciprut A, Baylancicek S, Sari M, Akdas F, Tutkun A (2007) Ototoxic effect of Burrow solution applied to the guinea pig middle ear. Otol Neurotol 28:605–608PubMedGoogle Scholar
  8. 8.
    Reregistration Eligibility Decision Document (1993) Boric acid and its sodium salts. EPA 738-R-93-017, U.S. Environmental Protection Agency, Office of Pesticide Programs, U.S.Government Printing Office, Washington, DCGoogle Scholar
  9. 9.
    World Health Organization (1998) Boron, environmental health criteria, 204; Geneva, SwitzerlandGoogle Scholar
  10. 10.
    Minja BM, Moshi NH, Ingvarsson L, Bastos I, Grenner J (2006) Chronic suppurative otitis media in Tanzanian school children and its effects on hearing. East Afr Med J 83:322–325PubMedGoogle Scholar
  11. 11.
    Ozcan MK, Ozcan M, Karaarslan A, Karaarslan F (2003) Otomycosis in Turkey: predisposing factors, aetiology and therapy. J Laryngol Otol 117:39–42PubMedCrossRefGoogle Scholar
  12. 12.
    Lundy LB, Graham MD (1993) Ototoxicity and ototopical medication: a survey of otolaryngologist. Am J Otol 14:141–146PubMedGoogle Scholar
  13. 13.
    Gates GA (2001) Safety of ofloxacin otic and other ototopical treatments in animal models and in humans. Pediatr Infect Dis J 20:104–107PubMedCrossRefGoogle Scholar
  14. 14.
    Goycoolea MV (1992) The round window membrane under normal and pathological conditions. Acta Otolaryngol Suppl 493:43–55PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Sedat Öztürkcan
    • 1
    • 3
  • Rıza Dündar
    • 1
  • Hüseyin Katılmış
    • 1
  • Ali Ekber İlknur
    • 1
  • Sinem Aktaş
    • 1
  • Senem Hacıömeroğlu
    • 2
  1. 1.Department of Otorhinolaryngology and Head and Neck SurgeryAtatürk Research and Training Hospital, Ministry of HealthIzmirTurkey
  2. 2.Laboratory Animal Breeding UnitBornova Veterinary Control and Research InstituteIzmirTurkey
  3. 3.İzmir Atatürk Eğitim ve Araştırma Hastanesi 2. KBB KliniğiIzmirTurkey

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