Archives of Gynecology and Obstetrics

, Volume 263, Issue 1–2, pp 2–6 | Cite as

Evaluating GA733-2 mRNA as a marker for the detection of micrometastatic breast cancer in peripheral blood and bone marrow

  • X. Y. Zhong
  • S. Kaul
  • A. Eichler
  • G. Bastert
ORIGINAL ARTICLE

Abstract

The GA733-2 gene encodes the epithelial glycoprotein 40, a homophilic cell-cell adhesion molecule, which is expressed on the surface of epithelial cells and associated with a variety of carcinomas, e.g. breast, colorectal and lung carcinomas. To test if it could serve as a tumor marker, we have analysed the expression of GA733-2 in bone marrow (BM) and peripheral blood (PB) from healthy donors, and of patients with haematological malignancies or breast cancer using reverse transcriptase-polymerase chain reaction (RT-PCR). The GA733-2 nested PCR was positive in 100% (8/8) of BM and 40% (16/40) of PB from healthy donors, in 100% (33/33) of BM from patients with breast cancer who had no evidence of distant metastases and also in BM and PB from patients with haematological malignancies. GA733-2 mRNA is not specific as a marker for the detection of breast cancer cells in BM and PB.

Key words Expression GA733-2 RT-PCR Bone marrow Peripheral blood 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • X. Y. Zhong
    • 1
  • S. Kaul
    • 2
  • A. Eichler
    • 2
  • G. Bastert
    • 2
  1. 1.Department of Obstetrics and Gynaecology, University Basel, CH-4031 Basel
  2. 2.Laboratory of Oncology, Department of Obstetrics and Gynaecology, University Hospital, Heidelberg, GermanyDE

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