Archives of Gynecology and Obstetrics

, Volume 300, Issue 6, pp 1741–1750 | Cite as

Endometrial expression of receptivity markers subject to ovulation induction agents

  • Alexander Freis
  • Ariane Germeyer
  • Julia Jauckus
  • Edison Capp
  • Thomas Strowitzki
  • Markus Zorn
  • Amanda Machado WeberEmail author
Gynecologic Endocrinology and Reproductive Medicine



Implantation rates differ according to ovulation induction agents in ART. This study investigates the different local endometrial effects of LH- versus hCG-induced ovulation.


Endometrial stromal cells from healthy patients were cultured with hCG or LH in different concentrations, supplemented with 250 ng/mL hCG and progesterone after 2 and 5 days. In addition after decidualization induction, cells were treated with hCG (50 or 250 ng/mL) or LH (10 or 50 ng/mL) for 3 days. Receptivity markers expression was evaluated by real-time quantitative PCR on day 3 and 6.


On day 3, non-decidualized cells treated with LH showed an increased expression of IGFBP1, IL-8 and CXCL12 compared to hCG. The expression pattern changed on day 6, where cells treated with hCG showed higher expression of implantation markers compared to LH-treated cells. Furthermore, on day 3, decidualized cells treated with hCG250 showed an increased IL8 and CXCL12 expression compared to LH10.


LH seems to modulate the local endometrial expression of receptivity markers earlier compared to hCG; however, the effect is not sustained over time in cells without prior decidualization. Though, in decidualized cells, pattern changed and an earlier positive effect of hCG was shown on IL-8 and CXCL12.


Endometrium implantation hCG LH IGFBP1 IL8 CXCL12 



Insulin-like growth factor binding protein 1


Interleukin 8


CXC-Motif-Chemokin ligand 1


CXC-Motif-Chemokin ligand 12, also stromal cell-derived factor 1 (SDF-1) or pre-B cell growth-stimulating factor (PBSF)


Luteinizing hormone


Human chorionic gonadotropin



We acknowledge financial support by Ruprecht-Karls-Universität Heidelberg for open-access publishing.

Author Contributions

Conceptualization, AF, AG, AMW; and JJ; methodology, AF, AG, JJ, AMW; formal analysis, AF, AG, EC, AMW; investigation, JJ, AMW, MZ, AF; resources, AG, AF, TS; data curation, JJ, AMW; writing—original draft preparation, AF, AMW; writing—review and editing, AF, AG, AMW, EC; visualization, AF, AMW; supervision, AG; project administration, AG; funding acquisition, AG, AF.


This research was funded by Dres. Majic/Majic-Schlez-Stiftung, University of Heidelberg.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest regarding this paper.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. All patients provided written consent for the use of their tissue samples. The study was approved by the Ethics Committee of Ruprecht Karls University Heidelberg (S239/2005).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Gynaecological Endocrinology and Fertility DisordersUniversity Hospital HeidelbergHeidelbergGermany
  2. 2.Department of Obstetrics and Gynecology, Medicine SchoolUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  3. 3.Central LaboratoryUniversity of HeidelbergHeidelbergGermany

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