The propolis and boric acid can be highly suitable, alone/or as a combinatory approach on ovary ischemia-reperfusion injury
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Ovarian ischemia-reperfusion (IR) damage continues to be a serious infertility problem. The oxidative stress plays central role in the development of IR injuries. Activation of antioxidants decreases IR injuries; however, the efficacy of antioxidant agents remains controversial. Unfortunately, there has been no evidence for medicinal use of boric acid (BA) and propolis (Prop) on ovarian IR injury on rats so far. This study will provide to reveal the potential applications of the Prop and BA in ovarian IR therapy.
The Sprague–Dawley rats were randomized into five groups: I—control, II—IR, 3 h of ischemia and 3 h of reperfusion, III and IV—a signal dose of oral BA (7 mg/kg) and Prop (100 mg/kg) alone 1 h before induction of IR, V—Prop and BA together 1 h before induction of IR. SOD (superoxide dismutase), CAT (catalase), GSH (glutathione), MPO (myeloperoxidase), MDA (malondialdehyde), and IL-6 (interleukin-6) levels were quantified by ELISA and the TNF-α (tumor necrosis factor-α), 8-OHdG (8-hydroxylo-2′-deoxyguanosin) and Caspase-3 expressions were performed by immunohistochemical analyses.
BA and Prop pretreatment significantly reduced MPO, MDA, and IL-6 levels and pathologic score in IR rats, with no effects in control group. These agents used in therapy also decreased TNF-α, 8-OHdG and Caspase-3 protein expressions increased by IR. Furthermore, BA and Prop combination showed significant ameliorative effects on ovary injury caused by IR through acting as an antioxidant, anti-inflammatory and antiapoptotic agent.
BA and Prop alone and especially in combination could be developed as therapeutic agents against ovary IR injury.
KeywordsIschemia-reperfusion Ovary Propolis Boric acid Oxidative stress/antioxidant activity Inflammation
FG and KK: performed the experiments, collected and analyzed the data and wrote the manuscript. HSE and SC: performed the experiments, and analyzed the data and performed the quality control of data and algorithms. HA and SAJ: collected the data. GE and MBD: participated in animal studies and related experiments. YSS: analyzed the data and revised the manuscript. All the authors read and approved the final manuscript.
This work was supported by the BAP from Atatürk University [Grant no. 2017/6014].
Compliance with ethical standards
Conflict of interest
All the authors declare that there is no conflict of interest.
This study was approved by the Atatürk University Local Ethics Committee for Animal Experiments (No. 129, 11.07.2016).
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