Comparison of two protocols of blastocyst biopsy submitted to preimplantation genetic testing for aneuploidies: a randomized controlled trial
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To compare the effectiveness of two protocols of blastocyst biopsy submitted to preimplantation genetic testing for aneuploidies (PGT-A).
This is a randomized controlled trial of a cohort of 221 patients undergoing PGT-A. 106 female patients aged ≤ 40 years with no less than 8 mature oocytes retrieved and ≥ 3 good-quality embryos on day 3 were randomly assigned to the day-3 hatching-based TE biopsy. The remaining 115 females aged ≤ 40 years with ≥ 8 MII oocytes obtained and no less than 3 high-quality embryos on day 3 were assigned to the TE biopsy without hatching group (also called the new biopsy group). The primary outcome was measured by a live birth after the first embryo transfer.
The live birth rate did not differ significantly between the two groups (50.00% vs. 59.26%, P > 0.05, OR 1.46; 95% CI 0.78–2.70). There was no significant between-group difference in the rates of implantation, clinical pregnancy, and miscarriage. However, the frozen blastocyst rate was significantly lower in the day-3 hatching-based TE biopsy compared with the new biopsy group (47.54% vs. 53.96%, P < 0.05, OR 1.29; 95% CI 1.08–1.56).
Our study provides strong evidence that the new blastocyst biopsy method exhibits advantages over day-3 hatching-based TE biopsy method. Using this method, we were able to obtain more blastocysts to perform trophectoderm biopsy in patients subjected to PGT-A.
KeywordsPreimplantation genetic testing for aneuploidies (PGT-A) Blastocyst biopsy Embryonic aneuploidy Next-generation sequencing Frozen embryo transfer
LH: data management/analysis, manuscript writing/editing, ZHB: data management/analysis, manuscript writing/editing, YGL: data collection, LM: manuscript editing, MSY: manuscript editing, ZHZ: data collection, WKL: study design, manuscript writing/editing.
This study is funded by National Natural Science Foundation of China (no. 81601256) and Merck Serono China Research Fund for Fertility.
Compliance with ethical standards
Conflict of interest
All the authors declare no conflict of interest with respect to the authorship and/or publication of this article.
All procedures performed in this study involving human participants were in accordance with the ethical standards of the Reproductive Medicine, Shandong University Research Ethics Committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in this study.
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