Using time-lapse technology to explore vacuolization in embryos on Day 3 and Day 4
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To investigate the occurrence and development state of embryo vacuoles between the 8-cell and morula stages, and to explore how vacuoles affected the development of embryos.
A retrospective study of a cohort of 422 patients undergoing conventional in vitro fertilization or intracytoplasmic sperm injection. With the help of time-lapse imaging, the development processes and outcomes of good quality embryos with or without vacuoles were analyzed.
Vacuole positive embryos had significantly lower blastulation rate and good quality blastulation rate than vacuole negative embryos, p < 0.05. Compared to vacuole negative embryos, the number of best and good quality blastocysts was significantly reduced, while the number of fair and discarded ones was significantly increased, p < 0.05. The average starting time of vacuolization was 73.7 ± 9.3 h after insemination. The proportion of blastomeres affected by vacuoles was associated with embryonic developmental potential.
Vacuolization on Day 3 and Day 4 was frequently observed and was detrimental to embryo development. The proportion of blastomeres affected by vacuoles may be an indicator of embryo developmental potential.
KeywordsTime-lapse Vacuoles Blastulation rate Blastocyst quality Developmental potential
ZJY: data management/analysis, manuscript writing/editing. ZWX: data management/analysis, manuscript writing/editing. LH: data collection, manuscript editing. ZHB: data collection, manuscript editing. LM: manuscript editing. MSY: manuscript editing. WKL: project development, manuscript writing/editing.
This study was funded by The National Key Research and Development Program of China (2017YFC1001000) and The Shanghai Commission of Science and Technology (17DZ2271100).
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The study was approved by the Ethics Committee of the Center for Reproductive Medicine, Shandong University.
Informed consent was obtained from all individual participants included in this study.
- 10.Kirkegaard K, Kesmodel US, Hindkjaer JJ, Ingerslev HJ (2013) Time-lapse parameters as predictors of blastocyst development and pregnancy outcome in embryos from good prognosis patients: a prospective cohort study. Hum Reprod 28(10):2643–2651. https://doi.org/10.1093/humrep/det300 CrossRefPubMedGoogle Scholar
- 11.Desai N, Ploskonka S, Goodman LR, Austin C, Goldberg J, Falcone T (2014) Analysis of embryo morphokinetics, multinucleation and cleavage anomalies using continuous time-lapse monitoring in blastocyst transfer cycles. Reprod Biol Endocrinol 12:54. https://doi.org/10.1186/1477-7827-12-54 CrossRefPubMedPubMedCentralGoogle Scholar
- 12.Basile N, Nogales Mdel C, Bronet F, Florensa M, Riqueiros M, Rodrigo L, Garcia-Velasco J, Meseguer M (2014) Increasing the probability of selecting chromosomally normal embryos by time-lapse morphokinetics analysis. Fertil Steril 101(3):699–704. https://doi.org/10.1016/j.fertnstert.2013.12.005 CrossRefPubMedGoogle Scholar
- 14.Minasi MG, Colasante A, Riccio T, Ruberti A, Casciani V, Scarselli F, Spinella F, Fiorentino F, Varricchio MT, Greco E (2016) Correlation between aneuploidy, standard morphology evaluation and morphokinetic development in 1730 biopsied blastocysts: a consecutive case series study. Hum Reprod 31(10):2245–2254. https://doi.org/10.1093/humrep/dew183 CrossRefPubMedGoogle Scholar
- 24.Wallbutton S, Kasraie J (2010) Vacuolated oocytes: fertilization and embryonic arrest following intra-cytoplasmic sperm injection in a patient exhibiting persistent oocyte macro vacuolization–case report. J Assist Reprod Genet 27(4):183–188. https://doi.org/10.1007/s10815-010-9399-2 CrossRefPubMedPubMedCentralGoogle Scholar