Preventative effect of celecoxib in dimethylbenz[a]anthracene-induced ovarian cancer in rats
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The present study investigated the preventive effect of the cyclooxygenase (COX)-2 inhibitor, celecoxib, in 7,12-dimethylbenz[a]anthracene (DMBA)-induced ovarian cancer in a rat model.
A diet containing celecoxib (1500 ppm) was started 2 weeks before the introduction of DMBA. DMBA-soaked cotton threads were surgically applied to induce ovarian cancer in female Wistar rats. Tumor growth and survival were observed for 24 weeks.
During the study period, an overall tumor incidence of 97.5% was observed and 65% of tumors were ovarian adenocarcinoma. The celecoxib diet significantly reduced the incidence and size of DMBA-induced ovarian cancers and significantly improved survival of tumor-bearing rats. The preventive effect of celecoxib was associated with increased apoptosis.
DMBA-induced ovarian cancer in rats recapitulates many pathophysiological features of the human counterpart. Our results provide supportive evidence that celecoxib has a preventive effect on development of ovarian cancer in a rat model.
KeywordsOvarian cancer 7, 12-dimethylbenz[a]anthracene (DMBA) Cyclooxygenase 2 inhibitor Celecoxib Survival
XC and SZ designed the study. SZ, WQ, ZT, JW and KY participated in animal studies and related experiments. SZ, WS and XC analyzed data and wrote the manuscript. All authors read and approved the final manuscript.
This research is supported by Foundations of Medicine and Hygiene Science and Technology Planning Project of Zhejiang Province (2012KYB031) and Zhejiang Natural Science Foundation (LY14H160010).
Compliance with ethical standards
This study was approved by the Biomedical Research Ethics Committee of Shanghai Institute for Biological Science of Chinese Academy.
Consent for publication
Conflict of interest
The authors declare no competing interests.
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