Increased ZNF84 expression in cervical cancer
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Little is known about ZNF84 gene. This study aims to investigate ZNF84 expression in cervical cancer (CC) and the effects of ZNF84 on CC.
Materials and methods
Cervical cancer tissue specimens were collected from The First People’s Hospital of Foshan. ZNF84 and Akt expression were detected by immunohistochemistry. The influence of ZNF84 on cell proliferation was detected by CCK-8 kits. The effects of ZNF84 on Akt protein and mRNA expression were detected by western blotting and qPCR, respectively.
High expression of ZNF84 protein (80.0%) was detected within CC tissues while negative expression was found in normal cervical tissues. ZNF84 was specifically associated with tumor size (p = 0.018) and negatively associated with other indicators. Further, in squamous cell carcinoma, ZNF84 was associated with both TNM staging (p = 0.041) and tumor size (p = 0.041). In vitro, we used shZNF84 to inhibit the mRNA and protein expression of ZNF84, and showed marked inhibition of cancer cell proliferation by shZNF84. Furthermore, inhibition of ZNF84 down-regulated Akt. Ly294002 (an Akt inhibitor) decreased the cell inhibition ability of shZNF84, indicating the involvement of Akt. Finally, the relationship between ZNF84 and Akt in vivo showed positive correlation (p = 0.023).
ZNF84 expression was increased in CC tissues and associated with tumor size. ZNF84 promoted cell proliferation which might involve Akt signal.
KeywordsZNF84 Cervical cancer Akt Cell proliferation
SW conceived and supervised the study; SW, PL, GZ and HG designed experiments; PL, HG, HS, ZL, XG, ZD and SL performed experiments; SZ and YW analyzed data; SW, PL and HG wrote the manuscript; SW made manuscript revisions.
This study was funded by Medical Science and Technology Research Fund of Guangdong province, China (A2017605, A2016208), by Science and Technology Planning Project of Guangdong Province, China (2016A020215147), by National Natural Science Foundation of China (81302244).
Compliance with ethical standards
Conflict of interest
The authors declared that they have no conflicts of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
This study is a retrospective, non-interventional research and the Medical Ethics Committee of The First People’s Hospital of Foshan has approved the waiver of patients’ informed consent. This study has been approved by the Ethics Committee of The First People’s Hospital of Foshan, China (Number: L 3).
- 8.Brebi P, Maldonado L, Noordhuis MG et al (2014) Genome-wide methylation profiling reveals zinc finger protein 516 (ZNF516) and FK-506-binding protein 6 (FKBP6) promoters frequently methylated in cervical neoplasia, associated with HPV status and ethnicity in a Chilean population. Epigenetics 9(2):308–317CrossRefPubMedGoogle Scholar
- 11.Zhang J, Cheng Y, Duan M et al (2017) Unveiling differentially expressed genes upon regulation of transcription factors in sepsis. Biotech 7(1):46–53Google Scholar