Archives of Gynecology and Obstetrics

, Volume 297, Issue 6, pp 1525–1532 | Cite as

Increased ZNF84 expression in cervical cancer

  • Peng Li
  • Hongsheng Guo
  • Guangji Zhou
  • Haiyan Shi
  • Zhen Li
  • Xiaodan Guan
  • Ziliang Deng
  • Shuxian Li
  • Shixiong Zhou
  • Yan Wang
  • Sen WangEmail author
Gynecologic Oncology



Little is known about ZNF84 gene. This study aims to investigate ZNF84 expression in cervical cancer (CC) and the effects of ZNF84 on CC.

Materials and methods

Cervical cancer tissue specimens were collected from The First People’s Hospital of Foshan. ZNF84 and Akt expression were detected by immunohistochemistry. The influence of ZNF84 on cell proliferation was detected by CCK-8 kits. The effects of ZNF84 on Akt protein and mRNA expression were detected by western blotting and qPCR, respectively.


High expression of ZNF84 protein (80.0%) was detected within CC tissues while negative expression was found in normal cervical tissues. ZNF84 was specifically associated with tumor size (p = 0.018) and negatively associated with other indicators. Further, in squamous cell carcinoma, ZNF84 was associated with both TNM staging (p = 0.041) and tumor size (p = 0.041). In vitro, we used shZNF84 to inhibit the mRNA and protein expression of ZNF84, and showed marked inhibition of cancer cell proliferation by shZNF84. Furthermore, inhibition of ZNF84 down-regulated Akt. Ly294002 (an Akt inhibitor) decreased the cell inhibition ability of shZNF84, indicating the involvement of Akt. Finally, the relationship between ZNF84 and Akt in vivo showed positive correlation (p = 0.023).


ZNF84 expression was increased in CC tissues and associated with tumor size. ZNF84 promoted cell proliferation which might involve Akt signal.


ZNF84 Cervical cancer Akt Cell proliferation 


Author contributions

SW conceived and supervised the study; SW, PL, GZ and HG designed experiments; PL, HG, HS, ZL, XG, ZD and SL performed experiments; SZ and YW analyzed data; SW, PL and HG wrote the manuscript; SW made manuscript revisions.


This study was funded by Medical Science and Technology Research Fund of Guangdong province, China (A2017605, A2016208), by Science and Technology Planning Project of Guangdong Province, China (2016A020215147), by National Natural Science Foundation of China (81302244).

Compliance with ethical standards

Conflict of interest

The authors declared that they have no conflicts of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

This study is a retrospective, non-interventional research and the Medical Ethics Committee of The First People’s Hospital of Foshan has approved the waiver of patients’ informed consent. This study has been approved by the Ethics Committee of The First People’s Hospital of Foshan, China (Number: L[2016] 3).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Histology and Embryology of Basic Medical DepartmentGuangdong Medical UniversityDongguanChina
  2. 2.Pathological DepartmentThe First People’s Hospital of FoshanFoshanChina

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