Archives of Gynecology and Obstetrics

, Volume 295, Issue 6, pp 1515–1522 | Cite as

Is there any correlation between oocyte polarization microscopy findings with embryo time lapse monitoring in ICSI program?

  • Azita Faramarzi
  • Mohammad Ali KhaliliEmail author
  • Azam Agha-Rahimi
  • Marjan Omidi
Gynecologic Endocrinology and Reproductive Medicine



The aim was to investigate the relationship between the presence of the meiotic spindle (MS) and zona pellucida (ZP) birefringence of MII oocytes with morphokinetics variables of derived embryos in ICSI setting.


Using a polarization imaging system, the ZP birefringence and presence of MS were evaluated pre ICSI. Also, morphokinetics variables including time of second PB extrusion (tPB2), time of pronuclei appearance (tPNa), time of pronuclei fading (tPNf), time of two to eight discrete cells (t2–t8) ECC1 (t2−tPB2), cc2a (t3−t2), S2 (t4−t3) and S3 (t8−t5) as well as irregular cleavage events of 368 embryos were analyzed with time lapse monitoring (TLM).


t5 occurred earlier in high birefringent ZP (HB-ZP) compared with low birefringent oocytes (LB-ZP; p = 0.001). In addition, t2 happened later in invisible MS compared to visible MS oocytes (p = 0.013). There were significantly lower rates of cell fusion (Fu) in oocytes with HB-ZP and also the Fu and trichotomous mitoses (TM) together in visible MS oocytes (p = 0.005, p = 0.001 and p = 0.001, respectively).


Both t2 and t5 timings and irregular cleavage events of embryos were correlated with ZP birefringence and MS status, respectively. So, combining the information from both oocyte polarization microscopy imaging and embryo TLM can be a useful tool for single embryo transfer (SET) program.


Oocyte quality Meiotic spindle Zona pellucida Time laps monitoring Embryo kinetics Irregular cleavage 


Compliance with ethical standards


This study was funded by Yazd research and clinical center for infertility.

Conflict of interest

All authors declare no conflict of interest.

Ethical approval

The present study conforms to the provisions of the Declaration of Helsinki. Also, this study was approved by the Institutional Review Board of the Yazd Research and Clinical Center for Infertility (IR.SSU.MEDICINE.REC.1395.2). The unique nature of identifying patients was completely preserved.

Informed consent

Informed consent was obtained from all participants included in the study.

Authors’ contribution

Azita Faramarzi: data collection, methodology, writing; Azam Agh-Rahimi: writing, data analysis; Marjan Omidi: language editing, data analysis; Mohammad Ali Khalili: leader of study, methodology, editing the paper.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Azita Faramarzi
    • 1
  • Mohammad Ali Khalili
    • 1
    Email author
  • Azam Agha-Rahimi
    • 1
  • Marjan Omidi
    • 1
  1. 1.Research and Clinical Center for InfertilityShahid Sadoughi University of Medical SciencesYazdIran

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