Is there any correlation between oocyte polarization microscopy findings with embryo time lapse monitoring in ICSI program?
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The aim was to investigate the relationship between the presence of the meiotic spindle (MS) and zona pellucida (ZP) birefringence of MII oocytes with morphokinetics variables of derived embryos in ICSI setting.
Using a polarization imaging system, the ZP birefringence and presence of MS were evaluated pre ICSI. Also, morphokinetics variables including time of second PB extrusion (tPB2), time of pronuclei appearance (tPNa), time of pronuclei fading (tPNf), time of two to eight discrete cells (t2–t8) ECC1 (t2−tPB2), cc2a (t3−t2), S2 (t4−t3) and S3 (t8−t5) as well as irregular cleavage events of 368 embryos were analyzed with time lapse monitoring (TLM).
t5 occurred earlier in high birefringent ZP (HB-ZP) compared with low birefringent oocytes (LB-ZP; p = 0.001). In addition, t2 happened later in invisible MS compared to visible MS oocytes (p = 0.013). There were significantly lower rates of cell fusion (Fu) in oocytes with HB-ZP and also the Fu and trichotomous mitoses (TM) together in visible MS oocytes (p = 0.005, p = 0.001 and p = 0.001, respectively).
Both t2 and t5 timings and irregular cleavage events of embryos were correlated with ZP birefringence and MS status, respectively. So, combining the information from both oocyte polarization microscopy imaging and embryo TLM can be a useful tool for single embryo transfer (SET) program.
KeywordsOocyte quality Meiotic spindle Zona pellucida Time laps monitoring Embryo kinetics Irregular cleavage
Compliance with ethical standards
This study was funded by Yazd research and clinical center for infertility.
Conflict of interest
All authors declare no conflict of interest.
The present study conforms to the provisions of the Declaration of Helsinki. Also, this study was approved by the Institutional Review Board of the Yazd Research and Clinical Center for Infertility (IR.SSU.MEDICINE.REC.1395.2). The unique nature of identifying patients was completely preserved.
Informed consent was obtained from all participants included in the study.
Azita Faramarzi: data collection, methodology, writing; Azam Agh-Rahimi: writing, data analysis; Marjan Omidi: language editing, data analysis; Mohammad Ali Khalili: leader of study, methodology, editing the paper.
- 5.Dar S, Lazer T, Shah PS, Librach CL (2014) Neonatal outcomes among singleton births after blastocyst versus cleavage stage embryo transfer: a systematic review and meta-analysis. Hum Repord Update 0:1–10Google Scholar
- 11.Khalili MA, Mojibian M, Sultan A-M (2005) Role of oocyte morphology on fertilization and embryo formation in assisted reproductive techniques. MEFS 10:72–77Google Scholar
- 19.Świątecka J, Anchim T, Leśniewska M, Wołczyński S, Bielawski T, Milewski R (2014) Oocyte zona pellucida and meiotic spindle birefringence as a biomarker of pregnancy rate outcome in IVF-ICSI treatment. Ginekol polska 1(85):264–271Google Scholar
- 29.Liu Y, Chapple V, Feenan K, Roberts P, Matson P (2015) Clinical significance of intercellular contact at the four-cell stage of human embryos, and the use of abnormal cleavage patterns to identify embryos with low implantation potential: a time-lapse study. Fertil Steril 103(6):1485–1491.e1CrossRefPubMedGoogle Scholar
- 33.Gardner DK, Weissman A, Howles CM, Shoham Z (2012) Textbook of assisted reproductive techniques fourth edition: volume 2: Clinical perspectives: CRC pressGoogle Scholar