Archives of Gynecology and Obstetrics

, Volume 292, Issue 5, pp 1083–1089 | Cite as

miR-31 functions as an oncogene in cervical cancer

  • Wenjing Zheng
  • Zhen Liu
  • Wei ZhangEmail author
  • Xiaoxia HuEmail author
Gynecologic Oncology



MicroRNAs are frequently altered in numerous cancers and are critical regulators of various diseases. miR-31 has been shown to be significantly altered in a variety of cancers.


In the present study, we measured the expression level of miR-31 in cervical cancer, CIN and normal cervical tissues by real-time RT (reverse transcription)-PCR. We also analyzed the correlations between the expression level of miR-31 and the clinical characteristics in cases of cervical squamous cell carcinoma. In addition, we measured the expression of miR-31 in cervical cancer cell lines, and transfected HPV16 E6 siRNA and HPV16 E7 siRNA into SiHa cells to investigate the effects on miR-31. Finally, the effects of miR-31 on cell proliferation, migration and invasion were measured in HeLa and SiHa cells that were transfected with a miR-31 mimic or a negative control.


We found that the expression level of miR-31 was significantly higher in cervical cancer patients than in normal individuals (P < 0.05). Aberrant expression of miR-31 was positively correlated with the lymph node metastasis (LNM), vessel invasion and HPV status (P < 0.05). Additionally, miR-31 was also overexpressed in the cervical cancer-derived HeLa and SiHa cells compared with C33A cells (P < 0.05). Moreover, a relationship was found between miR-31 expression and the HPV16 oncoproteins E6/E7. Furthermore, we found that the overexpression of miR-31 can promote cell proliferation and enhance the migration and invasion abilities of cancer cells.


Our results suggested that miR-31 plays an oncogenetic role in the development and progression of cervical cancer.


miR-31 Cervical cancer HPV Biological function 



This work was supported by National Natural Science Foundation of China (No. 81060219) and Guangxi Natural Science Foundation of China (2014GXNSFAA118266).

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of GynecologyRenmin Hospital of Wuhan UniversityWuhanChina
  2. 2.Department of GynecologyThe People Hospital of Guangxi Zhuang Autonomous RegionNanningChina

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