Archives of Gynecology and Obstetrics

, Volume 292, Issue 2, pp 459–463 | Cite as

Noninvasive assays of in vitro matured human oocytes showed insignificant correlation with fertilization and embryo development

  • Sareh Ashourzadeh
  • Mohammad Ali KhaliliEmail author
  • Marjan Omidi
  • Seyed Nooraldin Nematollahi Mahani
  • Seyed Mehdi Kalantar
  • Abbas Aflatoonian
  • Victoria Habibzadeh
Gynecologic Endocrinology and Reproductive Medicine



Recently, the upgrading of in vitro maturation (IVM) of human oocytes as a promising strategy has emerged in assisted reproductive technology (ART). The goal was to evaluate the correlation of the in vitro matured oocytes selected on the basis of the zona pellucida (ZP) birefringence and meiotic spindles (MS) detection with fertilization and subsequent embryo development in ICSI program.


A total of 168 immature oocytes [germinal vesicle (n = 140) and metaphase I (n = 28)] obtained from patients undergoing oocytes retrieval for ICSI. After in vitro culture for 24–40 h, 112 (67 %) oocytes reached to MII stage. Using a polarized microscopy, the presence of MS and ZP birefringence were assessed in matured oocytes, followed by ICSI performance.


The rates of fertilization in oocytes with spindles (51.3 %) were similar to that of the oocytes without spindles (50.7 %; P = 1.00). Moreover, the fertilization rates in high birefringence (HB) oocytes was not statistically different than oocytes with low birefringence (LB) (P = 0.44). The findings also showed that 64.9 % of the fertilized oocytes developed to embryos, in which 33.3 % were derived from spindle-detected oocytes. Regarding the ZP birefringence, 35.5 % of the embryos were derived from HB oocytes.


There were insignificant relationships between the MS detection and ZP birefringence score with the rates of fertilization and embryo development in IVM oocytes.


IVM ZP birefringence Meiotic spindles Fertilization Embryo development 



The authors would like to thank Dr. Nasim Tabibnejad for her English editing of the manuscript.

Conflict of interest



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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Sareh Ashourzadeh
    • 1
    • 2
  • Mohammad Ali Khalili
    • 1
    Email author
  • Marjan Omidi
    • 1
  • Seyed Nooraldin Nematollahi Mahani
    • 3
  • Seyed Mehdi Kalantar
    • 1
  • Abbas Aflatoonian
    • 1
  • Victoria Habibzadeh
    • 2
  1. 1.Research and Clinical Center for InfertilityShahid Sadoughi University of Medical SciencesYazdIran
  2. 2.Afzalipour Clinical Center for Infertility, Afzalipour HospitalKerman University of Medical SciencesKermanIran
  3. 3.Department of Anatomical Sciences, Afzalipour School of MedicineKerman University of Medical SciencesKermanIran

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