Archives of Gynecology and Obstetrics

, Volume 291, Issue 6, pp 1283–1288 | Cite as

Does sildenafil have protective effects against ovarian ischemia-reperfusion injury in rats?

  • Adnan IncebiyikEmail author
  • Ahmet Seker
  • Hakan Camuzcuoglu
  • Sezen Kocaslan
  • Aysun Camuzcuoglu
  • Nese Gul Hilali
  • Mehmet Vural
  • Abdullah Taskin
  • Nurten Aksoy
General Gynecology



The aim of this study was to evaluate the protective activity of sildenafil treatment against ischemia-reperfusion damage created experimentally in rat ovaries.


For this study, 42 female Wistar rats were used, and the rats were separated randomly into six groups consisting of seven rats each: sham, torsion, torsion-detorsion, torsion-detorsion + saline, torsion-detorsion + sildenafil 0.7 mg/kg and torsion-detorsion + sildenafil 1.4 mg/kg. With the exception of the sham group, an ovarian torsion procedure was implemented in all other groups for 2 h. Then, a detorsion procedure was implemented to the groups for 2 h, with the exception of the torsion group. Medications were given intraperitoneally, one-half hour before the detorsion procedure in the saline, 0.7 and 1.4 mg/kg sildenafil groups. Finally, 2 ml of blood samples was drawn for markers of oxidative stress, while the ovaries which were torsioned for the histological examination were extracted from all rats.


According to the histopathological damage scores, the least damage was seen in the sham group and the most damage was seen in the torsion-detorsion group. The sildenafil treatment appeared to be effective in decreasing tissue damage; however, there were no differences between the dosages. Additionally, it was determined that the oxidative stress levels were higher in the torsion-detorsion group, while the sildenafil treatment caused a significant decrease in the oxidative stress levels.


The results of the current study showed that the sildenafil treatment can be effective in preventing tissue damage and oxidative stress induced by the ischemia-reperfusion created in rat ovaries.


Adnexal torsion Ischemia-reperfusion injury Sildenafil Rat model 


Conflict of interest

The authors have stated explicitly that there are no with this any financial support or relationships that may pose potential conflict of interest in this article.


  1. 1.
    Parlakgumus HA, Aka Bolat F, Bulgan Kilicdag E, Simsek E, Parlakgumus A (2014) Atorvastatin for ovarian torsion: effects on follicle counts, AMH, and VEGF expression. Eur J Obstet Gynecol Reprod Biol 175:186–190CrossRefPubMedGoogle Scholar
  2. 2.
    Kato H, Kanematsu M, Uchiyama M, Yano R, Furui T, Morishige KI (2014) Diffusion-weighted imaging of ovarian torsion: usefulness of apparent diffusion coefficient (adc) values for the detection of hemorrhagic infarction. Magn Reson Med Sci 13:39–44CrossRefPubMedGoogle Scholar
  3. 3.
    Sak ME, Soydinc HE, Sak S, Evsen MS, Alabalik U, Akdemir F et al (2013) The protective effect of curcumin on ischemia-reperfusion injury in rat ovary. Int J Surg 11:967–970CrossRefPubMedGoogle Scholar
  4. 4.
    Ozkisacik SYM, Gursoy H, Culhaci N (2014) Does gradual detorsion protect the ovary against ischemia-reperfusion injury in rats? Pediatr Surg 30:437–440CrossRefGoogle Scholar
  5. 5.
    Kurtoglu E, Kokcu A, Danaci M (2013) Asynchronous bilateral ovarian torsion. a case report and mini review. J Pediatr Adolesc Gynecol 27:122–124CrossRefPubMedGoogle Scholar
  6. 6.
    Beheshtian A, Salmasi AH, Payabvash S, Kiumehr S, Ghazinezami B, Rahimpour S et al (2008) Protective effects of sildenafil administration on testicular torsion/detorsion damage in rats. World J Urol 26:197–202CrossRefPubMedGoogle Scholar
  7. 7.
    Tripathi AS, Mazumder PM, Chandewar AV (2014) Changes in the pharmacokinetic of sildenafil citrate in rats with Streptozotocin-induced diabetic nephropathy. J Diabetes Metab Disord 13:1–8CrossRefGoogle Scholar
  8. 8.
    Inan M, Uz YH, Kizilay G, Topcu TY, Sapmaz MM, Akpolat M et al (2013) Protective effect of sildenafil on liver injury induced by intestinal ischemia/reperfusion. J Pediatr Surg 48:1707–1715CrossRefPubMedGoogle Scholar
  9. 9.
    Shih PK, Cheng CM, Li HP, Huang MF, Chiu CW, Chen JX et al (2013) Pretreatment with sildenafil alleviates early lung ischemia-reperfusion injury in a rat model. J Surg Res 185:77–83CrossRefGoogle Scholar
  10. 10.
    Yildirim A, Ersoy Y, Ercan F, Atukeren P, Gumustas K, Uslu U et al (2010) Phosphodiesterase-5 inhibition by sildenafil citrate in a rat model of bleomycin-induced lung fibrosis. Pulm Pharmacol Ther 23:215–221CrossRefPubMedGoogle Scholar
  11. 11.
    Hsueh TY, Wu YT, Lin LC, Chiu AW, Lin CH, Tsai TH (2013) Herb-drug interaction of Epimedium sagittatum (Sieb. et Zucc.) maxim extract on the pharmacokinetics of sildenafil in rats. Molecules 18:7323–7335CrossRefPubMedGoogle Scholar
  12. 12.
    Aksin M, Incebiyik A, Vural M, Gul Hilali N, Camuzcuoglu A, Camuzcuoglu H et al (2014) Does a risky outcome of antenatal screening test indicate oxidative stress? J Matern Fetal Neonatal Med 27:1033–1037CrossRefPubMedGoogle Scholar
  13. 13.
    Kara M, Daglioglu YK, Kuyucu Y, Tuli A, Tap O (2012) The effect of edaravone on ischemia-reperfusion injury in rat ovary. Eur J Obstet Gynecol Reprod Biol 162:197–202CrossRefPubMedGoogle Scholar
  14. 14.
    Bozkurt S, Arikan DC, Kurutas EB, Sayar H, Okumus M, Coskun A et al (2012) Selenium has a protective effect on ischemia/reperfusion injury in a rat ovary model: biochemical and histopathologic evaluation. J Pediatr Surg 47:1735–1741CrossRefPubMedGoogle Scholar
  15. 15.
    Ergun Y, Koc A, Dolapcioglu K, Akaydin Y, Dogruer G, Kontas T et al (2010) The protective effect of erythropoietin and dimethylsulfoxide on ischemia-reperfusion injury in rat ovary. Eur J Obstet Gynecol Reprod Biol 152:186–190CrossRefPubMedGoogle Scholar
  16. 16.
    Sahin FK, Cosar E, Koken G, Toy H, Basarali K, Buyukbas S (2008) Protective effect of aprotinin on ischemia-reperfusion injury in rat ovary. J Obstet Gynaecol Res 34:794–800CrossRefPubMedGoogle Scholar
  17. 17.
    Sayyah MM, Rashidi MR, Kaseb GM, Rashtchizadeh N, Taghavi S, Ouladsahebmadarek E et al (2012) The effect of erythropoietin against oxidative damage associated with reperfusion following ovarian detorsion. Eur J Obstet Gynecol Reprod Biol 162:182–186CrossRefGoogle Scholar
  18. 18.
    Richalet JP, Gratadour P, Robach P, Pham I, Dechaux M, Joncquiert LA et al (2005) Sildenafil inhibits altitude-induced hypoxemia and pulmonary hypertension. Am J Respir Crit Care Med 171:275–281CrossRefPubMedGoogle Scholar
  19. 19.
    Choi DE, Jeong JY, Lim BJ, Chung S, Chang YK, Lee SJ et al (2009) Pretreatment of sildenafil attenuates ischemia-reperfusion renal injury in rats. Am J Physiol Renal Physiol 297:362–370CrossRefGoogle Scholar
  20. 20.
    Uzun H, Konukoglu D, Nuri MK, Ersoy EY, Ozcevik S, Yavuz N (2008) The effects of sildenafil citrate on ischemic colonic anastomotic healing in rats: its relationship between nitric oxide and oxidative stress. World J Surg 32:2107–2113CrossRefPubMedGoogle Scholar
  21. 21.
    Maretta MTŠ, Bujdoš M, Tóth Š Jr, Jonecová Z, Veselá J (2012) Alterations of epithelial layer after ischemic preconditioning of small intestine in rats. J Mol Histol 43:171–178CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Adnan Incebiyik
    • 1
    Email author
  • Ahmet Seker
    • 2
  • Hakan Camuzcuoglu
    • 1
  • Sezen Kocaslan
    • 3
  • Aysun Camuzcuoglu
    • 1
  • Nese Gul Hilali
    • 1
  • Mehmet Vural
    • 4
  • Abdullah Taskin
    • 5
  • Nurten Aksoy
    • 5
  1. 1.Department of Gynecology and ObstetricsHarran University School of MedicineSanlıurfaTurkey
  2. 2.Department of General Surgery, Faculty of MedicineHarran UniversitySanliurfaTurkey
  3. 3. Department of Pathology, Faculty of MedicineHarran UniversitySanliurfaTurkey
  4. 4. Department of Gynecology and Obstetrics, Faculty of MedicineMarmara UniversityIstanbulTurkey
  5. 5. Department of Biochemistry, Faculty of MedicineHarran UniversitySanliurfaTurkey

Personalised recommendations