Archives of Gynecology and Obstetrics

, Volume 288, Issue 3, pp 643–648

Detection of Human Papillomavirus in biopsies of patients with cervical cancer, and its association with prognosis

  • Carla Limeira Barreto
  • Danyelly Bruneska Gondim Martins
  • José Luiz de Lima Filho
  • Vera Magalhães
Gynecologic Oncology



To assess the detection of Human Papillomavirus (HPV) in cervical biopsies, and its association with clinical response and survival of patients with cervical cancer.


The presence of HPV was determined by polymerase chain reaction (nested PCR) of material from 183 paraffin blocks containing biopsies of patients with cervical cancer. Directives of the International Federation of Gynecology and Obstetrics (FIGO) were used for the clinical staging of all cases. Survival analysis was conducted with the Kaplan–Meier method and differences between the curves were calculated by the log-rank test.


Amplification was positive in 78 cases, negative in 96 cases and indeterminate in 9. The detection of HPV had no statistically significant effect on the survival of patients.


From amongst the variables analyzed, only HPV positivity and clinical staging influenced a complete response after completing treatment. However, clinical staging was the only factor associated with progression of the disease, with patients in the early stages presenting better overall survival rates (p < 0.001).


Invasive cervical cancer HPV PCR Prognosis 


  1. 1.
    Agência Internacional para Pesquisa do Câncer (2008) Estatística global do câncer, Organização Mundial da Saúde. Accessed 10 Jan 2012
  2. 2.
    Instituto Nacional de Câncer José de Alencar Gomes da Silva (2011) Estimativa 2012: Incidência de Câncer no Brasil. Ministério da Saúde. Accessed 10 Jan 2012
  3. 3.
    Forouzanfar MH, Foreman KJ, Delossantos AM, Lozano R, Lopez AD, Murray CJL, Naghavi M (2011) Breast and cervical cancer in 187 countries between 1980 and 2010: a systematic analysis. Lancet 378:1461–1484PubMedCrossRefGoogle Scholar
  4. 4.
    Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (2002) Papilomavírus Humano (HPV): Diagnóstico e Tratamento. Accessed 16 June 2010
  5. 5.
    Hellner K, Munger K (2011) Human Papillomaviruses as therapeutic targets in human cancer. J Clin Oncol 29:1785–1794PubMedCrossRefGoogle Scholar
  6. 6.
    Ho GYF, Bierman R, Beardsley NP, Chang CJ, Burk RD (1998) Natural history of cervicovaginal papillomavirus infection in young women. N Engl J Med 338(7):423–428PubMedCrossRefGoogle Scholar
  7. 7.
    Higgins GD, Uzelin DM, Philips GE, Davy M, Roder D, Burrell CJ (1991) Increased age and mortality associated with cervical carcinomas negative for human papillomavirus. Lancet 338(8772):910–913PubMedCrossRefGoogle Scholar
  8. 8.
    Riou G, Bourhis J, Favre M, Orth G, Jeannel D, Le Doussal V (1990) Association between poor prognosis in early-stage invasive cervical carcinomas and non-detection of HPV DNA. Lancet 335(8699):1171–1174PubMedCrossRefGoogle Scholar
  9. 9.
    Eifel PJ, Berek JS, Markman M (2011) Cancer of the cervix, vagina, and vulva. In: Devita VT Jr, Lawrence TS, Rosenberg SA (eds) Cancer principles and practice of oncology. Lippincott Williams and Wilkins, Philadelphia, pp 1311–1344Google Scholar
  10. 10.
    Ota T, Takeshima N, Tabata T, Hasumi K, Takizawa K (2007) Treatment of squamous cell carcinoma of the uterine cervix with radiation therapy alone: long-term survival, late complications, and incidence of second cancers. Brit J Cancer 97(8):1058–1062PubMedCrossRefGoogle Scholar
  11. 11.
    Husman AMR, Walboomers JMM, van den Brule AJC, Meijer CJLM, Snijders PJF (1995) The use of general primers GP5 and GP elongated at their 3′ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR. J Gen Virol 76:1057–1062CrossRefGoogle Scholar
  12. 12.
    Haws ALF, He Q, Rady PL, Zhang L, Grady J, Hughes TK et al (2004) Nested PCR with the PGMY09/11 and GP5+/6+ prime sets improves detection of HPV DNA in cervical samples. J Virol Methods 122:87–93CrossRefGoogle Scholar
  13. 13.
    Khan S, Jaffer NN, Khan MN, Rai MA, Shafiq M, Ali A et al (2007) Human papillomavirus subtype 16 is common in Pakistani women with cervical carcinoma. IJID 11:313–317PubMedGoogle Scholar
  14. 14.
    Haie-Meder C, Morice P, Castiglione M (2010) Cervical cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol 21(5s):37–40CrossRefGoogle Scholar
  15. 15.
    Cooke SL, Temple J, MacArthur S, Zahra MA, Tan LT, Crawford RAF et al (2011) Intra-tumor genetic heterogeneity and poor chemoradiotherapy response in cervical cancer. B J Cancer 104:361–368CrossRefGoogle Scholar
  16. 16.
    Howell-Jones R, Bailey A, Beddows S, Sargent A, Silva N, Wilson G et al (2010) Multi-site study of HPV type-specific prevalence in women with cervical cancer, intraepithelial neoplasia and normal cytology, in England. BJC 103:209–216PubMedCrossRefGoogle Scholar
  17. 17.
    Mayrand MH, Duarte-Franco E, Rodrigues I, Walter SD, Hanley J, Ferency A et al (2007) Human Papillomavirus DNA versus Papanicolaou screening tests for cervical cancer. N Engl J Med 357(16):1579–1588PubMedCrossRefGoogle Scholar
  18. 18.
    Huang HJ, Huang SL, Lin CY, Lin RW, Chao FY, Chen MY et al (2004) Human papillomavirus genotyping by polymerase chain reaction-based genechip method in cervical carcinoma treated with neoadjuvant chemotherapy plus radical surgery. Int J Gynecol Cancer 14:639–649PubMedCrossRefGoogle Scholar
  19. 19.
    Prétet JL, Jacquard AC, Carcopino X, Charlot JF, Bouhour D, Kantelip B et al (2007) Human Papillomavirus (HPV) genotype distribution in invasive cervical cancers in France: EDITH study. Int J Cancer 122:428–432CrossRefGoogle Scholar
  20. 20.
    Huang LW, Chao SL, Hwang JL (2004) Human Papillomavirus-31-related types predict better survival in cervical carcinoma. Cancer 100(2):327–334PubMedCrossRefGoogle Scholar
  21. 21.
    Fakhry C, Westra WH, Li S, Cmelak A, Ridge JA, Pinto H, Forastiere A, Gillison ML (2008) Improved survival for patients with human papillomavirus-positive head and neck squamous cell carcinoma in a prospective clinical trial. J Natl Cancer Inst 100:261–269PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Carla Limeira Barreto
    • 1
    • 4
  • Danyelly Bruneska Gondim Martins
    • 2
  • José Luiz de Lima Filho
    • 2
  • Vera Magalhães
    • 3
  1. 1.Oncology UnitHospital Universitário Oswaldo CruzRecifeBrazil
  2. 2.Keizo Asami Immunopathology LaboratoryUniversidade Federal de PernambucoRecifeBrazil
  3. 3.Post-graduation Program in Tropical MedicineUniversidade Federal de PernambucoRecifeBrazil
  4. 4.RecifeBrazil

Personalised recommendations