Archives of Gynecology and Obstetrics

, Volume 287, Issue 4, pp 797–801 | Cite as

Evaluation of subgroups of the human sperm hypoosmotic swelling test in normozoospermic male cases with recurrent fertilization failure: a prospective case-controlled study

  • Tülay Irez
  • Taner A. Usta
  • Galip Zebitay
  • Engin Oral
  • Hülya Senol
  • Sezai Sahmay
Reproductive Medicine

Abstract

Purpose

To compare subgroups of the human sperm hypoosmotic swelling test subgroups in both recurrent fertilization negative infertile cases with normal semen analysis and fertilization positive controls.

Methods

This was a prospective case-controlled study performed with normospermic 33 previously fertile male (secondary infertility) and 41 infertile men who had undergone two or three unsuccessful in vitro fertilization attempts. HOS test was investigated in 4 subgroups including HOS 1, HOS 2, HOS 3, and HOS 4 according to the degree of sperm tail swelling and compared between the two groups.

Results

Four subgroups were compared and statistical significance was demonstrated in HOS 1, HOS 3 and HOS 4 tests (p < 0.001) in fertile and infertile men. Highest HOS 1 and lowest HOS 4 grades were determined in Group A. However, no statistical significance was determined between two groups in HOS 2 test which was minimal swelling in sperm tails.

Conclusions

HOS 1, HOS 3 and HOS 4 subgroups of HOS test are reliable and useful methods providing important information regarding the sperm function. A high HOS test 1 grade plus a low HOS test 4 grade should suggest a fertility problem, despite a normal semen analysis. HOS test subgroups provide additional information in normospermic cases with unexplained infertility.

Keywords

Hypoosmotic swelling test Male infertility Hypoosmotic swelling test grades Fertilization failure 

Notes

Conflict of interest

We declare that we have no conflict of interest.

References

  1. 1.
    Rowe PH, Comhaire FH, Hargreave TB, Mellows HJ (1993) WHO manual for the standardized investigation and diagnosis of the infertile couple. Cambridge University Press, CambridgeGoogle Scholar
  2. 2.
    Tartagni M, Schonauer MM, Cicinelli E et al (2002) Usefulness of the hypo-osmotic swelling test in predicting pregnancy rate and outcome in couples undergoing intrauterine insemination. J Androl 23:498–502PubMedGoogle Scholar
  3. 3.
    Tanahatoe SJ, McDonnell J, Goverde AJ, Hompes PG, Lambalk CB (2009) Total fertilization failure and idiopathic subfertility. Reprod Biol Endocrinol 12:3CrossRefGoogle Scholar
  4. 4.
    Buch JP (1993) Advanced sperm function testing. Curr Opin Urol 3:503–508CrossRefGoogle Scholar
  5. 5.
    Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJ (1984) Development of an assay to asses the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 70:219–228PubMedCrossRefGoogle Scholar
  6. 6.
    Majumdar K, Rajagopalan G, Bupathy A (2001) A study of the hypo-osmotic swelling test of spermatozoa in both fertile and infertile male subjects and its relationship with the standard spermiogram. Indian J Urol 17:156–158Google Scholar
  7. 7.
    Chan SY, Wang C, Chan ST, Ho PC (1990) Differential evaluation of human sperm hypoosmotic swelling test and its relationship with the outcome of in vitro fertilization of human oocytes. Hum Reprod 5:84–88PubMedGoogle Scholar
  8. 8.
    Chan SY, Craft IL, Chan YM, Leong MK, Leong CK (1990) The hypoosmotic swelling test and cryosurvival of human spermatozoa. Hum Reprod 5:715–718PubMedGoogle Scholar
  9. 9.
    Chan SY, Wang C, Ng M, So WW, Ho PC (1988) Multivariate discriminant analysis of the relationship between the hypoosmotic swelling test and the in vitro fertilizing capacity of human sperm. Int J Androl 11:369–378PubMedCrossRefGoogle Scholar
  10. 10.
    WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction (1999). 4th ed, Cambridge University Press, CambridgeGoogle Scholar
  11. 11.
    Mordel N, Mor-Yosef S, Margalioth E, Shemesh A, Samueloff A, Schenker JG (1989) The human sperm hypoosmotic swelling test: its practical application and suggestions for improvement. Int J Fertil 34:355–358PubMedGoogle Scholar
  12. 12.
    Mordel N, Dano I, Epistein-Eldan M, Shemesh A, Schenker JG, Laufer N (1993) Novel parameters of human sperm hypoosmotic swelling test and their correlation to standard spermatogram, total motile sperm fraction, and sperm penetration assay. Fertil Steril 59:1276–1279PubMedGoogle Scholar
  13. 13.
    Check JH, Katsoff D, Check ML (2001) Some semen abnormalities may cause infertility by impairing implantation rather than fertilization. Med Hypotheses 56:653–657PubMedCrossRefGoogle Scholar
  14. 14.
    Check JH, Katsoff D, Check ML, Choe JK, Swenson K (2001) In vitro fertilization with intracytoplasmic sperm injection is an effective therapy for male factor infertility related to subnormal hypo-osmotic swelling test scores. J Androl 22:261–265PubMedGoogle Scholar
  15. 15.
    Cincik M, Ergur AR, Tutuncu L et al (2007) Combination of hypoosmotic swelling/eosin Y test for sperm membrane integrity evaluation: correlations with other sperm parameters to predict ICSI cycles. Arch Androl 53:25–28PubMedCrossRefGoogle Scholar
  16. 16.
    Hossain A, Osuamkpe C, Hossain S, Phelps JY (2010) Spontaneously developed tail swellings (SDTS) influence the accuracy of the hypo-osmotic swelling test (HOS-test) in determining membrane integrity and viability of human spermatozoa. J Assist Reprod Genet 27:83–86PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Tülay Irez
    • 1
  • Taner A. Usta
    • 2
  • Galip Zebitay
    • 3
  • Engin Oral
    • 1
  • Hülya Senol
    • 1
  • Sezai Sahmay
    • 1
  1. 1.Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, IVF-ET Unit, Cerrahpaşa School of MedicineIstanbul UniversityIstanbulTurkey
  2. 2.Department of Obstetrics and GynecologyBagcilar Education and Research HospitalIstanbulTurkey
  3. 3.Department of Obstetrics and GynecologySuleymaniye Education and Research HospitalIstanbulTurkey

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