Archives of Gynecology and Obstetrics

, Volume 286, Issue 5, pp 1261–1267 | Cite as

The impact of human papillomavirus (HPV) types 6, 11 in women with genital warts

  • Mahin Jamshidi
  • Mohammad Shekari
  • Abdol Azim Nejatizadeh
  • Keyanoush Malekzadeh
  • Mahnaz Baghershiroodi
  • Parivash Davudian
  • Farzaneh Dehghan
  • Froogh Jamshidi
Gynecologic Oncology

Abstract

Objective

Human papillomaviruses (HPV) are etiologically associated with the development of virtually all genital warts. HPV-6 and HPV-11 are the most commonly detected HPV genotypes, but at least 20 other HPV genotypes have occasionally been found in genital wart tissue specimens.

Study design

The aim of this study was to determine from 100 genital wart tissue specimens collected from female patients using multiplex gap-PCR technique the prevalence of various genital HPV among women with HPV genital warts in south of Iran. 100 genital wart tissue specimens were tested for the presence of HPV PG5/PG6 and also for HPV type using polymerase chain reaction (PCR).

Results

Based on the collected data, 73 (73 %) samples were detected positive for HPV DNA and 23 (23 %) samples out of 100 samples were detected negative for HPV DNA. 49 (49 %) and 67 (67 %) of patients were detected positive for HPV type 6 and 11, respectively. There was a significant association between marital status and HPV genotype 6 (OR = 0.51, 95 % CI = 0.37–0.70, P = 0.01). Nevertheless, no significant association was found between marriage and HPV genotype 11 (OR = 0.85, 95 % CI = 0.58–1, 24, P = 0.7). Similarly, this result was demonstrated, in combined marriage and HPV-general (OR = 0.80, 95 % CI = 0.62–0.05, P = 0.4).

Conclusion

Concerning the prevalence of HPV in our study, determination of genital HPV prevalence and multiple infections among the normal population of women of Hormozgan Province is recommended.

Keywords

Human papillomavirus Genital warts PCR Typing 

References

  1. 1.
    Sanjose S, Diaz M, Castellsague X, Clifford G, Bruni L, Munoz N, Bosch FX (2007) Worldwide prevalence and genotypes distribution of cervical human papillomavirus DNA in women with normal cytology: a meta-analysis. Lancet Infect Dis 7:453–459PubMedCrossRefGoogle Scholar
  2. 2.
    De Villiers EM, Fauquet C, Broker TR, Bernard H-U, Zur Hausen H (2004) Classification of papillomaviruses. Virology 324:17–27PubMedCrossRefGoogle Scholar
  3. 3.
    Cox JT (2006) Epidemiology and natural history of HPV. J Fam Pract (Suppl):3–9Google Scholar
  4. 4.
    Von Krogh G (2001) Management of anogenital warts (condylomata acuminata). Eur J Dermatol 11:598–603Google Scholar
  5. 5.
    Centres for Disease Control and Prevention (2006) Sexually transmitted diseases treatment guidelines. MMWR 55: 62–67Google Scholar
  6. 6.
    Gissmann L, Zur Hausen H (1980) Partial characterization of viral DNA from human genital warts (Condylomata acuminata). Int J Cancer 25:605–609PubMedCrossRefGoogle Scholar
  7. 7.
    Padel AF, Venning VA, Evans MF, Quantrill AM, Fleming KA (1990) Human papillomaviruses in anogenital warts in children: typing by in situ hybridization. BMJ 300:1491–1494PubMedCrossRefGoogle Scholar
  8. 8.
    Sugase M, Moriyama S, Matsukura T (1991) Human papillomavirus in exophytic condylomatous lesions on different female genital regions. J Med Virol 34:1–6PubMedCrossRefGoogle Scholar
  9. 9.
    Haws ALF, He Q, Rady PL, Zhang L, Grady J, Hughes TK, Stisser K, Konig R, Tyring SK (2004) Nested PCR with the PGMY09/11 and GP5 +/6 + primer sets improve detection of HPV DNA in cervical samples. J Virol Methods 122:87–93CrossRefGoogle Scholar
  10. 10.
    Munoz N, Bosch FX, Sanjosé S, Herrero R, Castellsagué X, Shah KV, Snijders PJF, Meijer CJLM (2003) Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Eng J Med 348:518–527CrossRefGoogle Scholar
  11. 11.
    Bosch FX, Lorincz A, Munoz N, Meijer CJLM, Shah KV (2002) The causal relation between human papillomavirus and cervical cancer. J Clin Pathol 55:244–265PubMedCrossRefGoogle Scholar
  12. 12.
    Montaldo C, Mastinu A, Quartuccio M, Piras V, Denotti G, Pisano E, Orrú G (2007) Detection and genotyping of human papillomavirus DNA in samples from healthy Sardinian patients: a preliminary study. J Oral Pathol Med 36:482–487PubMedCrossRefGoogle Scholar
  13. 13.
    Bezerra SJS, Gonçalves PC, Franco ES, Pinheiro AKB (2005) Perfil de mulheres portadoras de lesões cervicais por HPV quanto aos fatores de risco para câncer de colo de útero. J Bras Doenças Sex Transm 17:143–148Google Scholar
  14. 14.
    Iftner T, Villa LL (2003) Chapter 12: human papillomavirus technologies. J Natl Cancer Inst Monogr 31:80–88PubMedCrossRefGoogle Scholar
  15. 15.
    Giovannelli L, Lama A, Capra G, Giordano V, Arico P, Ammatuna P (2004) Detection of human papillomavirus DNA in cervical samples: analysis of the new PGMY-PCR compared to Hybrid Capture II and MY-PCR assay and two-step nested PCR assay. J Clin Microbiol 42:3861–3864PubMedCrossRefGoogle Scholar
  16. 16.
    Carestiato FN, Silva KC, Balthazar DS, Silva L, Marinho M, Oliveira LHS, Cavalvanti SMB (2006) Analysis of molecular biology techniques for the diagnosis of human papillomavirus infection and cervical cancer prevention. Rev Soc Bras Med Trop 39:428–432PubMedCrossRefGoogle Scholar
  17. 17.
    Gravitti PE, Peyton CL, Alessi TQ, Wheeler CM, Coutlee F, Hildesheim A, Schiffiman MH, Scott DR, Apple RJ (2000) Improved amplification of genital human papillomaviruses. J Clin Microbiol 38:357–361Google Scholar
  18. 18.
    Hubbard RA (2003) Human papillomavirus testing methods. Arch Pathol Lab Med 127:940–945PubMedGoogle Scholar
  19. 19.
    Kosel S, Burggraf S, Mommsen J, Engelhardt W, Olgemoller B (2003) Type-specific detection of human papillomaviruses in a routine laboratory setting—improved sensitivity and specificity of PCR and sequence analysis compared to direct hybridization. Clin Chem Lab Med 41:787–791PubMedCrossRefGoogle Scholar
  20. 20.
    Sotlar K, Diemer D, Dethleffs A, Hack Y, Stubner A, Vollmer N, Menton S, Menton M, Dietz K, Wallwiener D, Kandolf R, Bultmann B (2004) Detection and typing of human papillomavirus by E6 nested multiplex PCR. J Clin Microbiol 42:3176–3184PubMedCrossRefGoogle Scholar
  21. 21.
    Meijer CJ, Snijders PJ, Castle PE (2003) Clinical utility of HPV genotyping. Gynecol Oncol 103:12–17CrossRefGoogle Scholar
  22. 22.
    Astori G, Arzese A, Pipan C, de Villiers E-M, Botta GA (1997) Characterization of a putative new HPV genomic sequence from a cervical lesion using L1 consensus primers and restriction fragment length polymorphism. Virus Res 50:57–63PubMedCrossRefGoogle Scholar
  23. 23.
    Mendez F, Munoz N, Posso H, Molano M, Moreno V, van den Brule AJC, Ronderos M, Meijer C, Munoz A (2005) Cervical coinfection with human papillomavirus (HPV) types and possible implications for the prevention of cervical cancer by HPV vaccines. J Infect Dis 192:1158–1165PubMedCrossRefGoogle Scholar
  24. 24.
    Kleter B, van Doorn L-J, Schrauwen L, Molijn A, Sastrowijoto S, ter Schegguet J, Lindeman J, ter Harmsel B, Burger M, Quint W (1999) Development and clinical evaluation of a highly sensitive PCR-reverse hybridization line probe assay for detection and identification of anogenital human papillomavirus. J Clin Microbiol 37:2508–2517PubMedGoogle Scholar
  25. 25.
    Choi Y-D, Jung W–W, Nam J-H, Choi H-S, Park C-S (2005) Detection of HPV genotypes in cervical lesions by the HPV DNA chip and sequencing. Gynecol Oncol 98:369–375PubMedCrossRefGoogle Scholar
  26. 26.
    Fontaine V, Mascaux C, Weyn C, Bernis A, Celio N, Lefevre P, Kaufman L, Garbar C (2007) Evaluation of combined general primer mediated PCR sequencing and type-specific PCR strategies for determination of human papillomavirus genotypes in cervical cell specimens. J Clin Microbiol 45:928–934PubMedCrossRefGoogle Scholar
  27. 27.
    Lee SH, Vigliotti VS, Vigliotti JS, Pappu S (2007) Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories. Infect Agents Cancer 2:1–11CrossRefGoogle Scholar
  28. 28.
    Lin C-Y, Chao A, Yang Y-C, Chou H–H, Ho C-M, Lin R-W, Chang T-C, Chiou J-Y, Chao F-Y, Wang K-L, Chien T-Y, Hsueh S, Huang C–C, Chen C-J, Lai C-H (2008) Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR. J Clin Virol 42:361–367PubMedCrossRefGoogle Scholar
  29. 29.
    Bosch FX, Lorincz A, Munoz N, Meijer CJLM, Shah KV (2002) The causal relation between human papillomavirus and cervical cancer. J Clin Pathol 55:244–265PubMedCrossRefGoogle Scholar
  30. 30.
    Usubutun A, Alemany L, Kucukali T (2009) Human papillomavirus types in invasive cervical cancer specimens from Turkey. Int J Gynecol Pathol 28:541–548PubMedCrossRefGoogle Scholar
  31. 31.
    Bosch FX, Castellsague H, de Sanjose S (2008) HPV and cervical cancer: screening or vaccination. Br J Cancer 98:15–21PubMedCrossRefGoogle Scholar
  32. 32.
    Franco EL, Rohan TE, Villa LL (1999) Epidemiologic evidence and human papillomavirus infection as a necessary cause of cervical cancer. J Natl Cancer Inst 91:506–511PubMedCrossRefGoogle Scholar
  33. 33.
    Liaw KL, Hildesheim A, Burk RD et al (2001) A prospective study of human papillomavirus (HPV) type 16 DNA detection by polymerase chain reaction and its association with acquisition and persistence of other HPV types. J Infect Dis 183:8–15PubMedCrossRefGoogle Scholar
  34. 34.
    Gargiulo F, De Francesco MA, Schreiber C et al (2007) Prevalence and distribution of single and multiple HPV infections in cytologically abnormal cervical samples from Italian women. Virus Res 125:176–182PubMedCrossRefGoogle Scholar
  35. 35.
    Herrero R, Hildesheim A, Bratti C et al (2000) Population based study of human papillomavirus infection and cervical neoplasia in rural Costa Rica. J Natl Cancer Inst 92:464–474PubMedCrossRefGoogle Scholar
  36. 36.
    An HJ, Cho NH, Lee SY (2003) Correlation of cervical carcinoma and precancerous lesions with human papillomavirus (HPV) genotypes detected with the HPV DNA chip microarray method. Cancer 97:1672–1680PubMedCrossRefGoogle Scholar
  37. 37.
    Spinillo A, Dal Bello B, Gardella B et al (2009) Multiple human papillomavirus infection and high grade cervical intraepithelial neoplasia among women with cytological diagnosis of atypical squamous cells of undetermined significance or low grade squamous intraepithelial lesions. Gynecol Oncol 113:115–119PubMedCrossRefGoogle Scholar
  38. 38.
    Munger K, Werness B, Dyson N, Phelps W, Harlow E, Howley P (1989) Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product. EMBO J 8:4099–4105PubMedGoogle Scholar
  39. 39.
    Duggan MA, Inoue M, Mcgregor SE, Stuart GCE, Morris S, Poon VG, Schepansky A, Honore L (1994) A paired comparison of dot blot hybridization and PCR amplification for HPV testing of cervical scrapes interpreted as CIN 1. Eur J Gynaecol Oncol 15:178–187PubMedGoogle Scholar
  40. 40.
    Ardnt A, Nottelmann K, Brock J, Neumann OG (1994) Das invert papillom und seine assoziation mit dem humanen papillomavirus. HNO 42:670–676Google Scholar
  41. 41.
    Cutts FT, Franceschi S, Goldie S, Castellsague X, Sanjose S (2007) Human Papillomavirus and HPV vaccines: a review. Bull World Health Org 85:719–726PubMedCrossRefGoogle Scholar
  42. 42.
    Hamkar R, Mokhtari-Azad T (2002) Prevalence of various types of Human Papillomavirus among cervical cancer and normal biopsy specimens in the Mazandaran province of Iran. East Mediterr Health J 6:805–811Google Scholar
  43. 43.
    Langenberg A, Cone RW, McDougall J, Kiviat N, Corey L (1993) Dual infection with human papillomavirus in a population with overt genital condylomas. J Am Acad Dermatol 28:434–442PubMedCrossRefGoogle Scholar
  44. 44.
    Brown DR, Schroeder JM, Bryan JT, Stoler MH, Fife KH (1999) Detection of multiple human papillomavirus types in condylomata acuminata lesions from otherwise healthy and immunosuppressed patients. J Clin Microbiol 37:3316–3322PubMedGoogle Scholar
  45. 45.
    Garland SM, Marc S, Heather L, Sings M, James S, Lu R, Railkar E, Barr R, Haupt and Elmar A (2009) Natural history of genital warts: analysis of the placebo arm of 2 randomized phase III trials of a quadrivalent human Papillomavirus (types 6, 11, 16, and 18) vaccine. J Infect Dis 199:805–814Google Scholar
  46. 46.
    Clifford G, Franceschi S, Diaz M, Munoz N, Villa LL (2006) Chapter 3: HPV type-distribution in women with and without cervical neoplastic diseases. Vaccine 24S3:26–34CrossRefGoogle Scholar
  47. 47.
    Ball, Winder DM, Vaughan K, Hanna N, Levv J, Sterling JC, Stanlev MA, Goon PK (2011) Analyses of human papillomavirus genotypes and viral loads in anogenital warts. J Med Virol 838:1345–1350CrossRefGoogle Scholar
  48. 48.
    Muller EE, Chirwa TF, Lewis DA (2010) Human papillomavirus (HPV) infection in heterosexual South African men attending sexual health services: associations between HPV and HIV serostatus. Sex transm Infect 86(3):175–180PubMedCrossRefGoogle Scholar
  49. 49.
    Kordi Tamandani DM, Sobti RC, Shekari M, Kaur S, Huria A (2008) Impact of polymorphism in IL-1RA gene on the risk of cervical cancer. Arch Gynecol Obstet 277:527–533CrossRefGoogle Scholar
  50. 50.
    Sobti RC, Shekari M, Kordi Tamandani DM, Kaur P, Suri V, Huria A (2008) Effect of NBS1 gene polymorphism on the risk of cervix carcinoma in northern Indian population. J Biol Markers 23(3):133–139Google Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Mahin Jamshidi
    • 2
  • Mohammad Shekari
    • 1
    • 2
  • Abdol Azim Nejatizadeh
    • 1
    • 2
  • Keyanoush Malekzadeh
    • 1
  • Mahnaz Baghershiroodi
    • 1
  • Parivash Davudian
    • 2
  • Farzaneh Dehghan
    • 1
  • Froogh Jamshidi
    • 3
  1. 1.Research Center for Molecular MedicineHormozgan University of Medical SciencesBandar-AbbasIran
  2. 2.Infections Research CenterHormozgan University of Medical SciencesBandar-AbbasIran
  3. 3.Dermatology Private ClinicBandar-AbbasIran

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