Archives of Gynecology and Obstetrics

, Volume 285, Issue 6, pp 1633–1636 | Cite as

How to close a colpotomy? Barbed suture and conventional suture effects on soft tissue: an ex vivo pilot study

  • Chenchit Chayachinda
  • Andreas Hackethal
  • Hans-Rudolf Tinneberg
General Gynecology



To evaluate the effects of different suture materials and techniques on soft tissue in relation to defined tensions and time points.

Materials and methods

Two bovine intestine samples, 4 × 4 cm size and ~3 mm thickness, were adapted with interrupted and continuous techniques using three types of suture materials: Vicryl (polyglactin 910), PDS II (polydioxanone), and V-Loc 180 (knotless, barbed polyglyconate). Four stitches or loops 9 mm apart with three knots, and 10 mm end length were performed by one gynecologist. Forces were applied from 6 newtons (N) to 14 N continuously. Outcome measures included breakage of tissue, tearing of thread, and shortening of the end length of thread. They were evaluated immediately and then at first, third, and fifth minute.


Tissue breakage using No. 3/0 suture materials appears in the applied force from 10 N. polydioxanone causes more tissue tearing than polyglactin 910. The least to withstand tension is knotless polyglyconate. Interrupted stitches hold the sutured sites better than continuous stitches in all groups of threads. Shortening of the knotless polyglyconate end length by half took place with 6 N force.


Simulating reparation of colpotomy, the ex vivo study supports that polyglactin 910 appears better in holding soft tissue than polydioxanone and knotless polyglyconate.


Knotless polyglyconate Polyglactin 910 Polydioxanone Reparation Colpotomy 


Conflict of interest

All authors have no conflicts of interest or financial ties to disclose.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Chenchit Chayachinda
    • 1
  • Andreas Hackethal
    • 2
  • Hans-Rudolf Tinneberg
    • 2
  1. 1.Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj HospitalMahidol UniversityBangkokThailand
  2. 2.Department of Obstetrics and GynaecologyJustus Liebig University of GiessenGiessenGermany

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