Archives of Gynecology and Obstetrics

, Volume 282, Issue 2, pp 215–219 | Cite as

Smoking and AMH levels in women with normal reproductive history

  • Alexandros Dafopoulos
  • Konstantinos DafopoulosEmail author
  • Panagiotis Georgoulias
  • Georgios Galazios
  • Vassilios Limberis
  • Panagiotis Tsikouras
  • Nikolleta Koutlaki
  • Georgios Maroulis
Reproductive Medicine



To investigate the effect of age and smoking on the AMH levels in normal cycling healthy women with normal reproductive history.

Materials and methods

In 137 women, blood samples were taken on day 3 of a spontaneous cycle. Serum FSH, LH, E2, progesterone and AMH were measured in all blood samples. For the statistical analysis of the data, t test, Pearson’s correlation and linear regression analysis were performed.


Of 137 women (43%), 59 were smokers. Age was positively correlated with serum FSH and LH levels (r = 0.584, P < 0.001 and r = 0.330, P < 0.001, respectively) and negatively correlated with serum AMH levels (r = −0.882, P < 0.001). There were no significant differences in FSH, LH, E2, progesterone and AMH levels between smokers and non-smokers. Multiple stepwise linear regression analysis showed that in both smokers and non-smokers, age was the most significant determinant of AMH levels (r = −0.889, P < 0.001 and r = −0.944, P < 0.001, respectively), while smoking was not related to AMH levels.


Aging significantly decreases AMH levels in women with normal cycles and normal reproductive history, while smoking does not seem to have significant effects on AMH levels.


FSH AMH Age Smoking Premenopausal women 


Conflict of interest statement

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Alexandros Dafopoulos
    • 1
  • Konstantinos Dafopoulos
    • 2
    Email author
  • Panagiotis Georgoulias
    • 3
  • Georgios Galazios
    • 1
  • Vassilios Limberis
    • 1
  • Panagiotis Tsikouras
    • 1
  • Nikolleta Koutlaki
    • 1
  • Georgios Maroulis
    • 1
  1. 1.Department of Obstetrics and GynecologyDemocritus University of ThraceAlexandroupolisGreece
  2. 2.Department of Obstetrics and Gynecology, University Hospital of LarissaUniversity of ThessaliaLarissaGreece
  3. 3.Department of Nuclear MedicineUniversity of ThessaliaLarissaGreece

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