Archives of Gynecology and Obstetrics

, Volume 282, Issue 1, pp 55–61 | Cite as

A randomised controlled trial on melatonin and rosiglitazone for prevention of adhesion formation in a rat uterine horn model

  • Orhan Aksakal
  • Bulent Yilmaz
  • Tayfun Gungor
  • Levent Sirvan
  • Necdet Sut
  • Ismet Inan
  • Senol Kalyoncu
  • Leyla Mollamahmutoglu
General Gynecology



To investigate the effectiveness of melatonin and rosiglitazone in reducing postoperative adhesion formation in a rat uterine horn model.


Thirty non-pregnant female Wistar albino rats, weighing 180–220 g, were used as a model for postoperative adhesion formation. The rats were randomised into three groups after seven standard lesions were inflicted in a 2-cm segment of each uterine horn and lower abdominal sidewall using bipolar cauterisation. The rats were treated with 10 mg/kg, intraperitoneal melatonin, and 1 mg/kg per day peroral rosiglitazone. No medication was given to the control group. As much as 20 uterine horns of 10 rats were evaluated in each group. Extent, severity, and degree of the adhesions to the uterine horns and, inflammation and fibrosis scores (histopathologically) were evaluated after 2 weeks of the treatment.


There was no mortality in the groups and all of the rats recovered without incident after operation. Rosiglitazone group had lower adhesion scores [median (min–max ranges)] regarding extent, severity, and degree of the adhesions [0 (0–3), 0 (0–3) and 0 (0–3), respectively], which were significantly different (P < 0.001, P < 0.05 and P < 0.01, respectively) from those of the controls [1 (0–3), 2 (0–2) and 2 (0–3), respectively]; however, there were no statistically significant differences between rosiglitazone versus melatonin groups [1 (0–4), 2 (0–3) and 1 (0–3), respectively] and melatonin versus control groups. Moreover, no significant differences were determined between groups regarding histopathologic findings.


Rosiglitazone, but not melatonin, is effective in prevention of adhesion formation in a rat uterine horn model.


Adhesion prevention Melatonin Rat Rosiglitazone Uterine horn model 



Authors acknowledge the technical help of Serkan Caliskan, Laboratory Technician, from Hacettepe University Faculty of Medicine, Department of Physiology.

Conflict of interest statement



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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Orhan Aksakal
    • 1
  • Bulent Yilmaz
    • 1
    • 5
  • Tayfun Gungor
    • 1
  • Levent Sirvan
    • 2
  • Necdet Sut
    • 3
  • Ismet Inan
    • 4
  • Senol Kalyoncu
    • 1
  • Leyla Mollamahmutoglu
    • 1
  1. 1.Department of Obstetrics and GynecologyZekai Tahir Burak Women’s Health Education and Research HospitalAnkaraTurkey
  2. 2.Department of PathologyZekai Tahir Burak Women’s Health Education and Research HospitalAnkaraTurkey
  3. 3.Department of Biostatistics, Faculty of MedicineTrakya UniversityEdirneTurkey
  4. 4.Department of Obstetrics and GynecologyAnkara Education and Research HospitalAnkaraTurkey
  5. 5.KurtulusTurkey

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