Prevalance of Chlamydia trochomatis, Ureaplasma urealyticum and Mycoplasma hominis infections in the unexplained infertile women
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To prospectively investigate the prevalence of Chlamydia trachomatis (CT), Mycoplasma hominis (MH) and Ureaplasma urealyticum (UU) in the cervical canal and pouch of Douglas in unexplained infertile women and compare it to healthy controls in the Turkish population.
Materials and methods
A total of 31 women presenting with a history of infertility [n = 24 (77%) primary infertility, n = 7 (23%) secondary infertility] between 20 and 38 years of age and 31 women willing to have tubal ligation between 30 and 41 years of age were consecutively included into this study. Specimens were taken from intra-abdominal washings and from the cervical canal. CT, MH and UU were detected with polymerase chain reaction (PCR).
Results of 62 women were analyzed. None of the participants met the criteria for salpingitis during laparoscopy. The most common infection in the cervical canal in both groups was UU, which was detected in 13 cases of infertile patients and 11 controls (P = 0.602). Cervical chlamydial and mycoplasmic infection was detected in one case each in infertile and control patients. Neither MH nor UU were obtained from the pouch of Douglas in both groups. Only CT was present in peritoneal fluid of an infertile woman who had also a concomitant chlamydial infection in the cervical canal.
Demonstration of cervical colonization of CT by PCR may be a promising method for the detection of asymptomatic pelvic infection in patients with unexplained infertility. However, screening for MH and UU is not cost-effective due to similar low rates of detection.
KeywordsUnexplained infertility Ureaplasma Mycoplasma Chlamydia Laparoscopy PCR
- 1.Abele-Horn M, Wolff C, Dressel P, Zimmermann A, Vahlensieck W, Pfaff F, Ruckdeschel G (1996) Polymerase chain reaction versus culture for detection of Ureaplasma urealyticum and Mycoplasma hominis in the urogenital tract of adults and the respiratory tract of newborns. Eur J Clin Microbiol Infect Dis. 15:595–598PubMedCrossRefGoogle Scholar
- 2.Arena B, Casares M, Valentine BH, Cooke RP (1993) Evaluation of laparoscopy and cervical swab in the diagnosis of Chlamydia Trochomatis infection of female genital tract. Arch Obstet Gynecol 253:5–7Google Scholar
- 3.Centers for Disease Control and Prevention (1993) Sexually transmitted diseases treatment guidelines. MMWR 42(RR-14):75–78Google Scholar
- 9.Guven MA, Gunyeli I, Dogan M, Ciragil P, Bakaris S, Gul M (2005) The demographic and behavioural profile of women with cervicitis infected with Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum and the comparison of two medical regimens. Arch Gynecol Obstet 272:197–200PubMedCrossRefGoogle Scholar
- 11.Jaczek KH (1985) Genital Chlamydia trachomatis: detection, treatment, and patient education. Can Fam Phys 31:1861–1865Google Scholar
- 21.Thejils H, Rahm VA, Rosen G, Gnarpe H (1987) Correlation between Chlamydia infection and clinical evaluation, vaginal wet smear, and cervical swab test in female adolescents. Am J Obstet Gynecol 157:974–976Google Scholar
- 22.Tiitinen A, Surcel HM, Halttunen M, Birkelund S, Bloigu A, Christiansen G, Koskela P, Morrison SG, Morrison RP, Paavonen J (2006) Chlamydia trachomatis and other chlamydial heat shock protein60-spesific antibody and cell-mediated responses predict tubal factor infertility. Hum Reprod 21:1533–1538PubMedCrossRefGoogle Scholar