Archives of Gynecology and Obstetrics

, Volume 272, Issue 1, pp 7–12

Regulation of progesterone production in human term trophoblasts in vitro by CRH, ACTH and cortisol (prednisolone)

  • Udo Jeschke
  • Ioannis Mylonas
  • Dagmar-Ulrike Richter
  • Ingo Höcker
  • Volker Briese
  • Antonis Makrigiannakis
  • Klaus Friese
Original Article

Abstract

Background: In most mammals, onset of labor is accompanied with progesterone withdrawal. In humans, cortisol blockade of progesterone is a possible mechanism involved in the initiation of labor. Therefore, aim of the study was to clarify the effect of CRH, ACTH and cortisol (prednisolone) on the release of progesterone by term trophoblast cells in vitro. Methods: Cytotrophoblast cells were prepared from human term placentas by standard dispersion of villous tissue followed by a percoll gradient centrifugation step. Trophoblasts were incubated with CRH, ACTH as well as with prednisolone Results: The release of progesterone is decreased in CRH- and ACTH-treated trophoblast cell cultures compared to untreated trophoblast cells. Addition of prednisolone in varying concentrations leads to an increase of trophoblast progesterone production. Conclusions: The results suggest that CRH and ACTH directly modulate the endocrine function of trophoblasts in culture by downregulating progesterone production. Prednisolone on the other hand showed a stimulating effect on progesterone production in term trophoblast cells in vitro. Because blockade of progesterone is a possible mechanism involved in initiation of labor, we may speculate that CRH and ACTH are directly involved in the auto- or paracrine regulation of this procedure.

Keywords

CRH ACTH Prednisolone Progesterone production Trophoblast cells 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Udo Jeschke
    • 1
  • Ioannis Mylonas
    • 1
  • Dagmar-Ulrike Richter
    • 2
  • Ingo Höcker
    • 2
  • Volker Briese
    • 2
  • Antonis Makrigiannakis
    • 3
  • Klaus Friese
    • 1
  1. 1.1st Department of Obstetrics and GynaecologyLudwig-Maximilians-University of MunichMunichGermany
  2. 2.Department of Obstetrics and GynecologyUniversity of RostockRostockGermany
  3. 3.Department of Obstetrics & Gynocology, Medical SchoolUniversity of CreteHeraklionGreece

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