Archives of Dermatological Research

, Volume 293, Issue 7, pp 356–362 | Cite as

Reconstruction of basement membrane in skin equivalent; role of laminin-1

  • Jae Youn Yi
  • Yong Ha Yoon
  • Hyun Sook Park
  • Chun Ho Kim
  • Chang Hwan Kim
  • Hyun Joo Kang
  • EunAh Lee
  • Youn Young Kim
  • Yong Jae Jin
  • Tae Hwan Kim
  • Y. S. Son
Original paper

Abstract To reconstruct the basement membrane in a skin equivalent, the epidermodermal interface was coated with porcine type IV collagen and mouse laminin-1 at various ratios before keratinocyte seeding. Laminin-1, a component of the basement membrane, induced massive infiltration of keratinocytes into the dermal equivalent, while type IV collagen induced discrete demarcation between dermal and epidermal compartments without any infiltrating cells. Immunohistochemical staining indicated that the laminin-induced infiltrating cells expressed endogenous type IV collagens at the cell periphery, which were not incorporated into the basement membrane structure. The infiltrating cells did not express fibronectin receptor α5β1 integrin but showed MMP-9 secretion and cell surface associated MMP-2. However, when laminin-1 was preincubated with type IV collagen, laminin-1-induced keratinocyte infiltration as well as MMP-9 induction were almost completely suppressed to basal levels. Therefore, replenishment of the type IV collagen lattice seemed to cause laminin-stimulated cells to anchor to the lattice, in a similar manner to the basal cells on the basement membrane of normal skin. Our study suggests that the molar ratio of basement membrane components may determine the behavior of basal cells within the wound healing microenvironment, which is probably regulated either by extracellular matrix deposition or degradation.

Keywords Laminin-1 Type IV collagen Basement membrane Cell migration Skin equivalent 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Jae Youn Yi
    • 1
  • Yong Ha Yoon
    • 1
  • Hyun Sook Park
    • 1
  • Chun Ho Kim
    • 1
  • Chang Hwan Kim
    • 1
  • Hyun Joo Kang
    • 1
  • EunAh Lee
    • 1
  • Youn Young Kim
    • 1
  • Yong Jae Jin
    • 1
  • Tae Hwan Kim
    • 1
  • Y. S. Son
    • 1
  1. 1.Laboratory of Tissue Engineering, Korea Cancer Center Hospital, 215-4, Gong Neung Dong, Nowon-Ku, Seoul, 139-706, Korea e-mail: ysson@kcchsun.kcch.re.kr, Tel.: +822-9701317, Fax: +822-9770381KR

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