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Archives of Dermatological Research

, Volume 291, Issue 10, pp 542–547 | Cite as

Alginate oligosaccharides stimulate VEGF-mediated growth and migration of human endothelial cells

  • A. Kawada
  • N. Hiura
  • S. Tajima
  • H. Takahara
Original Paper

Abstract Alginate oligosaccharides cleaved from alginic acid polysaccharides of seaweed were tested to determine their ability to enhance proliferation and migration of human umbilical vein endothelial cells. A mixture of alginate oligosaccharides (5 μg/ml in culture broth) stimulated endothelial cell growth, [3H]thymidine uptake and migration in the presence of recombinant vascular endothelial growth factor 165 (VEGF165). In contrast, a high concentration mixture of the oligosaccharides (∼100 μg/ml) suppressed cell growth. The stimulatory activity was comparable to that of heparin, with affinity to VEGF165, and decreased on heparin-induced stimulation. Each effective oligosaccharide had guluronic acid at the reducing end. A mixture of alginate oligosaccharides (5 μg/ml) and the most ¶effective fraction (1 μg/ml) stimulated endothelial cell migration. In the presence of VEGF and heparin, some alginate oligosaccharides with the peripheral guluronic acid demonstrated marked stimulatory effects, and one fraction also showed a migratory effect. These findings indicate novel activities of alginate oligosaccharide(s) in endothelial cell growth and migration and suggest synergistic and/or stabilizing effects on VEGF165-dependent stimulation of endothelial cells.

Key words Alginate oligosaccharide Endothelial cell Proliferation Migration Vascular endothelial growth factor 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • A. Kawada
    • 1
  • N. Hiura
    • 2
  • S. Tajima
    • 3
  • H. Takahara
    • 4
  1. 1.Department of Dermatology, Kinki University School of Medicine, 377-2, Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan Tel.: +81-723-660221, Fax: +81-723-682120JP
  2. 2.Maruha Corporation Central Research Institute, Ibaraki, JapanJP
  3. 3.Department of Dermatology, National Defense Medical College, Saitama, JapanJP
  4. 4.Department of Bioresource Science, School of Agriculture, Ibaraki University, Ibaraki, JapanJP

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