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Archives of Dermatological Research

, Volume 292, Issue 7, pp 325–332 | Cite as

Induction of tumor peptide-specific cytotoxic T cells under serum-free conditions by mature human dendritic cells

  • H. Jonuleit
  • A. Giesecke
  • A. Kandemir
  • L. Paragnik
  • J. Knop
  • A. H. Enk
Original Paper

Abstract Tumor vaccination strategies using antigen-pulsed dendritic cells (DC) are currently under development. We established an in vitro system using cultured DC from HLA-typed volunteers for the induction of tumor peptide-specific CD8+ T cells. The strength and specificity of the resulting CTL responses were investigated. For stimulation of syngeneic CD8+ T cells two well-defined DC populations were generated: CD1a+ immature DC cultured in the presence of GM-CSF and IL-4 and mature CD83+ DC generated by additional stimulation with a cytokine cocktail. Stimulations were performed under serum-free conditions and in the absence of exogenous cytokines. Analysis of T cell responses showed that mature DC, but not immature DC, were able to induce the expansion of syngeneic tumor peptide-specific CD8+ T cells. Priming of CD8+ T cells with peptide-pulsed mature DC rapidly increased the frequency of antigen-specific T cells (ELISPOT technique). T cells induced by mature DC showed strong antigen-specific cytotoxicity in 51Cr-release assays whereas no antigen-specific cytotoxicity was detectable in CTL generated by immature DC. These data show that terminally differentiated mature DC are necessary for the induction of tumor antigen-specific CTL responses.

Key words Dendritic cells Tumor-specific CTL Cytotoxicity Immunotherapy Dendritic cell ¶differentiation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • H. Jonuleit
    • 1
  • A. Giesecke
    • 1
  • A. Kandemir
    • 1
  • L. Paragnik
    • 1
  • J. Knop
    • 1
  • A. H. Enk
    • 1
  1. 1.Department of Dermatology, University of Mainz, Langenbeckstr. 1, 55101 Mainz, Germany e-mail: jonuleit@hautklinik.klinik.uni-mainz.de, Tel.: +49-6131-173541, Fax: +49-6131-17473541DE

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