The main pathology involved in chronic idiopathic urticaria (CIU) is immunological dysfunction which mainly adapts to the immune system of body. Pseudomonas aeruginosa-mannose-sensitive hemagglutinin (PA) is an inactivated Pseudomonas aeruginosa biological product which displays a broad immune regulatory effect. The current study was designed to explore the protective nature of PA as an immune regulator in CIU. The participants were randomly divided into CIU + PA, CIU, control + PA and control group. lg E, anti FcεRI, anti IgE antibody, IL-4, IL-17, TGF-β1 and interferon-γ in the sera were assayed by ELISA. Then CD4+ T cells and CD19+ B cells were isolated from peripheral blood of patients with CIU (n = 10) and healthy control (n = 10). CD4+ T cells and CD19+ B proliferation and apoptosis were analyzed through CCK-8 and flow cytometry respectively. T helper cells differentiations were assessed by real-time PCR. The results revealed that compared with the control group, the curative effect of CIU + PA group was more effective than that of the CIU control group. There was a hyper proliferation of CD19+ B cells in the CIU patients. Moreover, it was also discovered that presence of Th1 decreased while Th2 cells increased in CIU patients. PA significantly inhibited the proliferation of CD19+ B and Th2 cells but at the same time promoted the proliferation of Th1 compared to healthy control. The conclusion arrived at from this study is that the PA displayed a remarkable regulatory effect in CD4+ T cells and CD19+ B cells function by promoting Th1 but inhibited Th2 and the hyperfunction of B cells of CIU patients.
Chronic idiopathic urticaria Cytokines PA-MSHA T cells
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All authors participated in the design, interpretation of the studies and analysis of the data and review of the manuscript; XJ, SC, ZX and WA conducted the experiment. SC wrote the manuscript.
Compliance with ethical standards
Conflict of interest
The author(s) declare that they have no conflict of interest.
The study was approved by the Institutional Ethic Committee of No.3 Hospital of Shandong (3SDH-2016-190).
Informed consent was obtained from all individual participants included in the study.
Asero R (2017) Towards a better categorization of patients with chronic urticaria. Br J Dermatol 177:903–904CrossRefGoogle Scholar
Puxeddu I, Pratesi F, Ribatti D et al (2017) Mediators of inflammation and angiogenesis in chronic spontaneous urticaria: are they potential biomarkers of the disease? Mediat Inflamm 2017:4123694CrossRefGoogle Scholar
Tonacci A, Billeci L, Pioggia G et al (2017) Omalizumab for the treatment of chronic idiopathic urticaria: systematic review of the literature. Pharmacotherapy 37:464–480CrossRefGoogle Scholar
Eckman JA, Hamilton RG, Gober LM et al (2008) Basophil phenotypes in chronic idiopathic urticaria in relation to disease activity and autoantibodies. J Invest Dermatol 128:1956–1963CrossRefGoogle Scholar
Lourenço FD, Azor MH, Santos JC et al (2008) Activated status of basophils in chronic urticaria leads to interleukin-3 hyper-responsiveness and enhancement of histamine release induced by anti-IgE stimulus. Br J Dermatol 158:979–986CrossRefGoogle Scholar
Vonakis BM, Saini SS (2005) Basophils and mast cells in chronic idiopathic urticaria. Curr Allergy Asthma Rep 5:270–276CrossRefGoogle Scholar
Zweiman B, Valenzano M, Atkins PC (1998) Modulation of serum histamine releasing activity in chronic idiopathic urticaria. Immunopharmacology 39:225–234CrossRefGoogle Scholar
Abd El-Azim M, Abd E-A (2011) Chronic autoimmune urticaria: frequency and association with immunological markers. J Investig Allergol Clin Immunol 21:546–550PubMedGoogle Scholar
Wang L, Wu G, Qin X et al (2015) Expression of Nodal on bronchial epithelial cells influenced by lung microbes through DNA methylation modulates the differentiation of T-helper cells. Cell Physiol Biochem 37:2012–2022CrossRefGoogle Scholar
Qin L, Peng D, Hu C et al (2014) Differentiation of Th subsets inhibited by nonstructural proteins of respiratory syncytial virus is mediated by ubiquitination. PLoS ONE 9:e101469CrossRefGoogle Scholar
Qin L, Qiu K, Hu C, Wang L, Wu G, Tan Y (2019) Respiratory syncytial virus promoted the differentiation of Th17 cells in airway microenvironment through activation of Notch-1/Delta3. J Med Microbiol 68:649–656CrossRefGoogle Scholar
Tan Y, Liu H, Yang H et al (2013) An inactivated Pseudomonas aeruginosa medicament inhibits airway allergic inflammation and improves epithelial functions. J Physiol Sci 63:63–69CrossRefGoogle Scholar
Wei Y, Liu D, Jin X et al (2016) PA-MSHA inhibits the growth of doxorubicin-resistant MCF-7/ADR human breast cancer cells by downregulating Nrf2/p62. Cancer Med 5:3520–3531CrossRefGoogle Scholar
Zhao XM, Pan SY, Huang QL et al (2016) PA-MSHA in combination with EGFR tyrosine kinase inhibitor: a new strategy to overcome the drug resistance of non-small cell lung cancer cells. Oncotarget 7:49384–49396PubMedPubMedCentralGoogle Scholar
Boonpiyathad T, Sangasapaviliya A (2017) Hydroxychloroquine in the treatment of anti-histamine refractory chronic spontaneous urticaria, randomized single-blinded placebo-controlled trial and an open label comparison study. Eur Ann Allergy Clin Immunol 49:220–224CrossRefGoogle Scholar
Li L, Liu M, Kang L et al (2016) HHEX: a crosstalker between HCMV infection and proliferation of VSMCs. Front Cell Infect Microbiol 6:169PubMedPubMedCentralGoogle Scholar
Baker DL, Nakamura GR, Lowman HB et al (2016) Evaluation of IgE antibodies to omalizumab (Xolair®) and their potential correlation to anaphylaxis. AAPS J 18:115–123CrossRefGoogle Scholar
Kay AB, Clark P, Maurer M et al (2015) Elevations in T-helper-2-initiating cytokines (interleukin-33, interleukin-25 and thymic stromal lymphopoietin) in lesional skin from chronic spontaneous ('idiopathic') urticaria. Br J Dermatol 172:1294–1302CrossRefGoogle Scholar
Gao J, Yang A, Chen M et al (2011) mRNA profiles of cytokine receptors in unstimulated peripheral blood mononuclear cells from patients with chronic idiopathic urticaria. J Biomed Res 25:141–147CrossRefGoogle Scholar
Chen WC, Chiang BL, Liu HE et al (2008) Defective functions of circulating CD4+CD25+ and CD4+CD25− T cells in patients with chronic ordinary urticaria. J Dermatol Sci 51:121–130CrossRefGoogle Scholar
Sun L, Erxun K, Li J et al (2014) Correlations between anti-mast cell autoantibodies and chronic idiopathic urticaria. Ann Dermatol 26:145–149CrossRefGoogle Scholar
Sabroe RA, Fiebiger E, Francis DM et al (2002) Classification of anti-FcepsilonRI and anti-IgE autoantibodies in chronic idiopathic urticaria and correlation with disease severity. J Allergy Clin Immunol 110:492–499CrossRefGoogle Scholar