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Archives of Dermatological Research

, Volume 311, Issue 7, pp 563–571 | Cite as

Alterations in IL-4, IL-10 and IFN-γ levels synergistically decrease lipid content and protein expression of FAS and mature SREBP-1 in human sebocytes

  • Jihye Shin
  • Kun-pyo Kim
  • Hee Yoon Ahn
  • Bongjoon Kim
  • Yunhi ChoEmail author
Concise Communication
  • 118 Downloads

Abstract

When anti-acne alternatives from dietary and plant sources are ingested, systemic alterations of interleukin (IL)-4, IL-10, IL-12 and interferon (IFN)-γ, individually or simultaneously, are induced at a 0.1–10.0-fold (×) range of normal physiological concentrations (1×). However, little is known about the effects of these cytokines on excess sebum, a pathophysiological factor of acne development. In this study, human sebocytes were treated with 0.1–10.0× of IL-4, IL-10, IL-12 and IFN-γ for 3 or 5 days to elucidate the effects on lipid content. Treatment with individual cytokines decreased the lipid content at specific concentrations rather than in a concentration-dependent manner. Specifically, 5.0× of IL-4, 5.0× of IFN-γ (5.0IFN), and 0.5×, 5.0× and 10.0× of IL-10 for 3 days, and 0.5× of IL-4 (0.5IL4) for 5 days decreased lipid content to 87.6–93.0% of the control. Treatment with other concentrations of IL-4, IL-10 and IFN-γ, and 0.1–10.0× of IL-12 did not alter lipid content. Combined treatment with 0.5IL4, 5.0IFN and 0.5× of IL-10 for 3 or 5 days decreased the lipid content more than each individual treatment. However, this effect was more evident after 3 days, in parallel with decreased levels of triglycerides, cholesterol esters and free fatty acids, the major lipid compositions of sebocytes, and decreased protein expression of fatty acid synthase (FAS) and mature sterol response element-binding protein-1 (SREBP-1), the lipogenesis-related factors, without altered cell proliferation. We demonstrated that suppressed IL-4 and IL-10 with enhanced IFN-γ synergistically decreased lipid content and protein expression of FAS and mature SREBP-1 in human sebocytes.

Keywords

Sebocytes Cytokines Synergism Lipid content FAS Mature SREBP-1 

Notes

Acknowledgements

This study was supported by CJ Foods R & D Center, CJ CheilJedang Corporation in Suwon-si, Gyeongggi-do, Republic of Korea (KHU Grant No. 20150943).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Medical Nutrition, Graduate School of East–West Medical ScienceKyung Hee UniversityYongin-siRepublic of Korea
  2. 2.CJ Foods R & D CenterCJ CheilJedang CorporationSuwon-siRepublic of Korea

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