Activators of SIRT1 in wound repair: an animal model study
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Caloric restriction (CR) and resveratrol activate SIRT1 and induce anti-inflammatory and antioxidant properties. We perform excisional lesion on the dorsum of four groups anesthetized animals: ad libitum-AL diet, AL diet with topical application of 2% resveratrol-Rv, 30% calorie restricted, and finally 30% calorie restricted with 2% resveratrol and we examine CR and Rv effects in wound repair. Restricted animals remained with CR for 31 days. The lesion was performed on day 18 of CR, and resveratrol application was started on day 19. Lesion samples were then collected on days 3 and 10 of treatment for structural, morphometric, and protein analyses. Our results showed that CR and Rv group as well as R group had enhanced numbers of blood vessels, VEGF, fibroblast, birefringent collagen fiber areas in the lesion. We conclude that effects in wound repair suggests that both CR and resveratrol may modulate angiogenesis, fibroplasia, and collagenesis, which could be ascribed to the action of SIRT1.
KeywordsResveratrol Caloric restriction Tissue repair SIRT1 Skin Collagen
The authors are grateful to the students Amanda Tomazella, Bruna Assunção Bechtold, Leandro de Souza Rodrigues, and Rafaela Bresciani Rossi, who were responsible for the care of the animals and the collection of tissues on the day of euthanasia.
This work was supported by the PROPesq-Uniararas (Pesquisa Institucional Uniararas).
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
All the surgical and experimental procedures employed in this study were performed according to the norms established by the Brazilian College of Animal Experimentation (COBEA) and were approved by the Animal Use Ethics Committee (CEUA) of UNIARARAS (protocol number 023/2014) and conducted according with the Guide for the Care and Use of Laboratory Animals .
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