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Downregulation of aquaporin 3 in bullous pemphigoid patients

  • Mona Mostafa Korany
  • Marwa Ahmed Amer
  • Laila Ahmed Rashed
  • Marwah Adly Saleh
Original Paper
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Abstract

Bullous pemphigoid (BP) is a chronic autoimmune skin disease. Aquaporin 3 (AQP 3) has a possible role in the pathogenesis of many dermatological diseases. In this work, we aimed to evaluate the expression of AQP 3 in BP. Perilesional skin biopsies were taken from 24 BP patients and 13 controls. The biopsies were stained by direct immunofluorescence using rabbit anti-human AQP 3 FITC antibody. The expression of AQP 3 was weak in 5 patients (20.8%), moderate in 18 patients (75%), strong in 1 patient (4.2%) in the suprabasal layers. It was negative in 4 patients (16.7%), weak in 18 patients (75%), moderate in 2 patients (8.3%) and no strong fluorescence was seen in the basal layers. In the controls, the expression was strong in ten controls (76.9%), moderate in three controls (23.1%) and no controls showed weak fluorescence in the suprabasal layer. The basal layer showed strong fluorescence in 11 controls (84.6%), moderate in 2 controls (15.4%) and no controls showed mild or no fluorescence. There was a statistically significant difference in the expression of AQP 3 between basal and suprabasal layers of BP patients but not of the controls. There was statistically significant difference in the expression of AQP 3 between patients and controls in both the basal (P value < 0.001) and the suprabasal layers (P value < 0.001). In conclusion, AQP 3 was downregulated in BP patients especially in the basal cell layer. This suggests that AQP 3 plays a role in the pathogenesis of BP.

Keywords

Aquaporin 3 Basal Bullous pemphigoid Epidermis Immunofluorescence Suprabasal 

Notes

Funding

None.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Mona Mostafa Korany
    • 1
  • Marwa Ahmed Amer
    • 1
  • Laila Ahmed Rashed
    • 2
  • Marwah Adly Saleh
    • 1
  1. 1.Department of DermatologyFaculty of Medicine Cairo UniversityCairoEgypt
  2. 2.Department Biochemistry and Molecular BiologyFaculty of Medicine Cairo UniversityCairoEgypt

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