Archives of Dermatological Research

, Volume 310, Issue 2, pp 127–138 | Cite as

Increased senescent CD8+ T cells in the peripheral blood mononuclear cells of Behçet’s disease patients

  • Ji Young Yang
  • Mi Jin Park
  • Sun Park
  • Eun-So Lee
Original Paper
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Abstract

Behçet’s disease (BD) is a chronic inflammatory disease characterized by recurrent mucocutaneous, ocular, and skin lesions. Immunosenescence is associated with increased susceptibility to infection and chronic low grade inflammation. This study aimed to investigate the differences in the frequencies of immunosenescent cells in the peripheral blood mononuclear cells (PBMCs) of patients with BD. PBMCs were isolated from age-matched patients with active BD (n = 19), inactive BD (n = 20), disease controls (DCs, n = 15) and healthy controls (HCs, n = 15). The frequencies of senescent CD4+ T cells (CD3+ CD4+ CD27− CD28− cells), CD8+ T cells (CD3+ CD8+ CD27− CD28− cells) and B cells (CD19+ CD27− IgD− cells) were analyzed using flow cytometry. Senescence-associated β galactosidase activity was also measured in CD8+ T cells using flow cytometry with 5-dodecanoylaminofluorescein di-β-d-galactopyranoside. Frequencies of senescent CD4+ and CD19+ cells were not significantly different between the groups. The frequency of senescent CD8+ T cells was significantly higher in active BD than in DCs and HCs. C-reactive protein and erythrocyte sedimentation rate levels, which indicate disease activity, did not correlate with increased frequencies of immunosenescent cells. Steroid treatment, specific organ involvement, and HLA-B51 status did not have a significant influence on the frequencies of immunosenescent cells. Frequencies of senescence-associated β galactosidase+ CD8+ T cells were significantly higher in active BD and inactive BD compared to DCs and HCs. There was an increased frequency of senescent CD8+ T cells in the PBMCs of patients with BD.

Keywords

Behcet’s disease Immunosenescence Lymphocytes Flow cytometry β-Galactosidase 
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Notes

Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) (NRF-2016R1A2B1008803).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study protocol was approved by the Ajou Institutional Review Board (IRB No.: AJIRB-BMR-GEN-14-462) prior to study commencement and was in accordance with the Declaration of Helsinki.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Ji Young Yang
    • 1
  • Mi Jin Park
    • 1
  • Sun Park
    • 2
  • Eun-So Lee
    • 1
  1. 1.Department of DermatologyAjou University School of MedicineSuwonSouth Korea
  2. 2.Department of Microbiology and ImmunologyAjou University School of MedicineSuwonSouth Korea

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