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Archives of Dermatological Research

, Volume 310, Issue 1, pp 77–83 | Cite as

5α-Reductase isozymes and aromatase mRNA levels in plucked hair from young women with female pattern hair loss

  • P. Sánchez
  • C. Serrano-Falcón
  • J. M. Torres
  • S. SerranoEmail author
  • E. OrtegaEmail author
Original Paper

Abstract

Female pattern hair loss (FPHL) is an important hair disorder, especially when young women are affected. However, pharmacological treatments are not successful in all women. Androgens, especially dihydrotestosterone (DHT), may play a role in FPHL, but many women with this disorder have normal serum androgen levels. It therefore appears that hair follicle levels of DHT depend on in situ testosterone (T) metabolism. Because T can be converted to DHT or estradiol (E2) by 5α-reductase (5α-R) and aromatase, respectively, these enzymes would determine DHT and E2 concentrations and their ratio. We propose and apply a low-invasive, sensitive and precise method for the absolute quantification of mRNA levels of aromatase and 5α-R isozymes (type 1, type 2 and type 3) in plucked hair from young women with FPHL. Normoandrogenic women with FPHL and controls were studied. Plucked hair samples were obtained by trichogram from vertex scalp and mRNA levels quantified by real-time RT-PCR. We revealed for the first time the presence of 5α-R3 mRNA in human hair. Interestingly, one, two, or even three 5α-R isozymes were increased in some women with FPHL but not in others, which may explain the lack of response to 5α-R inhibitors in some FPHL cases. Aromatase mRNA levels were significantly lower in women with FPHL than in controls. It may therefore produce a reduction in oestrogen levels and an increase in the androgen/oestrogen ratio in hair. The proposed low-invasive technique offers a molecular aetiologic diagnosis of FPHL for the selection of more appropriate pharmacological treatments with early predicted effectiveness.

Keywords

Female pattern hair loss Aromatase 5α-R isozymes mRNA levels Trichogram 

Notes

Acknowledgements

The authors thank R. Davies for revising the English text.

Compliance with ethical standards

Funding

This study was supported in part by the Andalusian Regional Government (Endocrinology and Metabolism Group).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures involving human participants were in accordance with the ethical standards of the national research committee and the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individuals who participated in the study.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular Biology, Faculty of MedicineUniversity of GranadaGranadaSpain
  2. 2.Hospital of GuadixGranadaSpain
  3. 3.Department of Dermatology, Faculty of MedicineUniversity of GranadaGranadaSpain

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