Archives of Dermatological Research

, Volume 309, Issue 5, pp 381–388 | Cite as

Effect of sinapic acid on hair growth promoting in human hair follicle dermal papilla cells via Akt activation

  • Hyunju Woo
  • Seungjun Lee
  • Seungbeom Kim
  • Deokhoon Park
  • Eunsun Jung
Original Paper


Hair loss known as alopecia is caused by abnormal hair follicle cycling including shortening of the anagen (growth) phase and changing of hair follicle morphology with miniaturization. In accordance with the life extension, the quality of life is considered to be a most important thing. The yearning for healthy and beautiful hair and low self esteem due to hair loss had negative influence on the quality of life with psychosocial maladjustment. The objective of this research was to identify new compound that can be used as a drug to promote hair growth. We investigated whether the function of sinapic acid (SA) is able to promote hair growth in human hair follicle dermal papilla cells (hHFDPC). We showed that treatment of SA in hHFDPC could induce proliferation and the activation of Akt signaling in HFDPC. In addition, SA could stimulate the expressions of the several growth factors, insulin-like growth factor 1, and vascular endothelial growth factor for hair growth. We showed that SA led to an increased level of phospho-GSK-3β and β-catenin accumulation in HFDPC. Finally, the promoting effect of SA in hHFDPC cell growth occurred by the induction of cell cycle progression. These results suggest that SA could be one of the potential candidate compounds for the treatment of alopecia by inducing hair growth through triggering the expressions of growth factors via activation of Akt and subsequent inactivation of GSK-3β /β-catenin pathway.


Human hair follicle derma papilla cells Sinapic acid Hair growth Wnt/β-catenin signaling pathway 



This research was supported by a Grant (A004600326) from Jeju Economic Region Diagram Industry R&D project Program funded by Jeju Province.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest


  1. 1.
    Kikuzaki H, Hisamoto M, Hirose K, Akiyama K, Taniguchi H (2002) Antioxidant properties of ferulic acid and its related compounds. J Agric Food Chem 50:2161–2168CrossRefPubMedGoogle Scholar
  2. 2.
    Yun KJ, Koh DJ, Kim SH, Park SJ, Ryu JH, Kim DG, Lee JY, Lee KT (2008) Anti-inflammatory effects of sinapic acid through the suppression of inducible nitric oxide synthase, cyclooxygase-2, and proinflammatory cytokines expressions via nuclear factor-kappaB inactivation. J Agric Food Chem 56:10265–10272CrossRefPubMedGoogle Scholar
  3. 3.
    Yoon BH, Jung JW, Lee JJ, Cho YW, Jang CG, Jin C, Oh TH, Ryu JH (2007) Anxiolytic-like effects of sinapic acid in mice. Life Sci 81:234–240CrossRefPubMedGoogle Scholar
  4. 4.
    Cherng YG, Tsai CC, Chung HH, Lai YW, Kuo SC, Cheng JT (2013) Antihyperglycemic action of sinapic acid in diabetic rats. J Agric Food Chem 61:12053–12059CrossRefPubMedGoogle Scholar
  5. 5.
    Shin DS, Kim KW, Chung HY, Yoon S, Moon JO (2013) Effect of sinapic acid against dimethylnitrosamine-induced hepatic fibrosis in rats. Arch Pharm Res 36:608–618CrossRefPubMedGoogle Scholar
  6. 6.
    Urysiak-Czubatka I (2014) Assessment of the usefulness of dihydrotestosterone in the diagnostics of patients with androgenetic alopecia. Postepy Dermatol Alergol 31: 207–215CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Rushton DH (2002) Nutritional factors and hair loss. Clin Exp Dermatol 27:396–404CrossRefPubMedGoogle Scholar
  8. 8.
    Trost LB, Bergfeld WF, Calogeras E (2006) The diagnosis and treatment of iron deficiency and its potential relationship to hair loss. J Am Acad Dermatol 54:824–844CrossRefPubMedGoogle Scholar
  9. 9.
    Harries MJ, Meyer KC, Paus R (2009) Hair loss as a result of cutaneous autoimmunity: frontiers in the immunopathogenesis of primary cicatricial alopecia. Autoimmun Rev 8:478–483CrossRefPubMedGoogle Scholar
  10. 10.
    Mounsey AL, Reed SW (2009) Diagnosing and treating hair loss. Am Fam Physician 80:356–362PubMedGoogle Scholar
  11. 11.
    Blumeyer A, Tosti A, Messenger A, Reygagne P, Del Marmol V, Spuls PI, Trakatelli M, Finner A, Kiesewetter F, Trüeb R, Rzany B, Blume-Peytavi U (2011) Evidence-based (S3) guideline for the treatment of androgenetic alopecia in women and in men. J Dtsch Dermatol Ges 9(Suppl. 6):S1–S57CrossRefPubMedGoogle Scholar
  12. 12.
    Rathnayake D, Sinclair R (2010) Male androgenetic alopecia. Expert Opin Pharmacother 11: 1295–1304CrossRefPubMedGoogle Scholar
  13. 13.
    Murad S, Walker LC, Tajima S, Pinnell SR (1994) Minimal structural requirements for minoxidil inhibition of lysyl hydroxylase in cultured fibroblasts. Arch Biochem Biophys 308:42–47CrossRefPubMedGoogle Scholar
  14. 14.
    Price VH (1999) Treatment of hair loss. N Engl J Med 341:964–973CrossRefPubMedGoogle Scholar
  15. 15.
    Lin CM, Li Y, Ji YC, Keng H, Cai XN, Zhang JK (2008) Microencapsulated human hair dermal papilla cells: a substitute for dermal papilla? Arch Dermatol Res 300:531–535CrossRefPubMedGoogle Scholar
  16. 16.
    Elliott K, Stephenson TJ, Messenger AG (1999) Differences in hair follicle dermal papilla volume are due to extracellular matrix volume and cell number: implications for the control of hair follicle size and androgen responses. J Invest Dermatol 113:873–877CrossRefPubMedGoogle Scholar
  17. 17.
    Stenn KS, Paus R (2001) Controls of hair follicle cycling. Physiol Rev 81:449–494PubMedGoogle Scholar
  18. 18.
    Stewart CE, Rotwein P (1996) Growth, differentiation, and survival: multiple physiological functions for insulin-like growth factors. Physiol Rev 76:1005–1026PubMedGoogle Scholar
  19. 19.
    Tavakkol A, Elder JT, Griffiths CE, Cooper KD, Talwar H, Fisher GJ, Keane KM, Foltin SK, Voorhees JJ (1992) Expression of growth hormone receptor, insulin-like growth factor 1 (IGF-1) and IGF-1 receptor mRNA and proteins in human skin. J Invest Dermatol 99:343–349CrossRefPubMedGoogle Scholar
  20. 20.
    Yano K, Brown LF, Detmar M (2001) Control of hair growth and follicle size by VEGF-mediated angiogenesis. J Clin Invest 107:409–417CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Bassino E, Gasparri F, Giannini V, Munaron L (2015) Paracrine crosstalk between human hair follicle dermal papilla cells and microvascular endothelial cells. Exp Dermatol 24:388–390CrossRefPubMedGoogle Scholar
  22. 22.
    Junlatat J, Sripanidkulchai B (2014) Hair growth-promoting effect of Carthamus tinctorius floret extract. Phytother Res 28:1030–1036CrossRefPubMedGoogle Scholar
  23. 23.
    Lee GS, Hong EJ, Gwak KS, Park MJ, Choi KC, Choi IG, Jang JW and Jeung EB (2010) The essential oils of Chamaecyparis obtuse promote hair growth through the induction of vascular endothelial growth factor gene. Fitotoerapia 81:17–24CrossRefGoogle Scholar
  24. 24.
    Rho SS, Park SJ, Hwang SL, Lee MH, Kim CD, Lee IH, Chang SY, Rang MJ (2005) The hair growth promoting effect of Asiasari radix extract and its molecular regulation. J Dermatol Sci 38:29–97CrossRefGoogle Scholar
  25. 25.
    Kishimoto J, Burgeson RE, Morgan BA (2000) Wnt signaling maintains the hair-inducing activity of the dermal papilla. Genes Dev 14:1181–1185PubMedPubMedCentralGoogle Scholar
  26. 26.
    Shimizu H, Morgan BA (2004) Wnt signaling through the beta-catenin pathway is sufficient to maintain, but not restore, anagen-phase characteristics of dermal papilla cells. J Invest Dermatol 122:239–245CrossRefPubMedGoogle Scholar
  27. 27.
    Xiong Y, Liu Y, Song Z, Hao F, Yang X (2014) Identification of Wnt/β-catenin signaling pathway in dermal papilla cells of human scalp hair follicles: TCF4 regulates the proliferation and secretory activity of dermal papilla cell. J Dermatol 41:84–91CrossRefPubMedGoogle Scholar
  28. 28.
    Huh S, Lee J, Jung E, Kim SC, Kang JI, Lee J, Kim, YW, Sung YK, Kang HK, Park D (2009) A cell-based system for screening hair growth-promoting agents. Arch Dermatol Res 301:381–385CrossRefPubMedGoogle Scholar
  29. 29.
    Kim YH, Proust JJ, Buchholz MJ, Chrest FJ, Nordin AA (1992) Expression of the murine homologue of the cell cycle control protein p34cdc2 in T lymphocytes. J Immunol 149:17–23PubMedGoogle Scholar
  30. 30.
    Tong T, Kim N, Park T (2015) Topical application of oleuropein induces anagen hair growth in telogen mouse skin. PLoS ONE 10: e0129578CrossRefPubMedPubMedCentralGoogle Scholar
  31. 31.
    Stacey DW (2003) Cyclin D1 serves as a cell cycle regulatory switch in actively proliferating cells. Curr Opin Cell Biol 15:158–163CrossRefPubMedGoogle Scholar
  32. 32.
    Resnitzky D, Gossen M, Bujard H, Reed SI (1994) Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system. Mol Cell Biol 14:1669–1679CrossRefPubMedPubMedCentralGoogle Scholar
  33. 33.
    Weeren PC, Bruyn KM, Vries-Smits AM, Lint J, Burgering BM (1998) Essential role for protein kinase B (PKB) in insulin-induced glycogen synthase kinase 3 inactivation. Characterization of dominant-negative mutant of PKB. J Biol Chem 273:13150–13156CrossRefPubMedGoogle Scholar
  34. 34.
    Cross DA, Alessi DR, Cohen P, Andjelkovich M, Hemmings BA (1995) Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature 378:785–789CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Hyunju Woo
    • 1
  • Seungjun Lee
    • 1
  • Seungbeom Kim
    • 1
  • Deokhoon Park
    • 1
  • Eunsun Jung
    • 1
  1. 1.Biospectrum Life Science InstituteSeongnam-siRepublic of Korea

Personalised recommendations