Archives of Dermatological Research

, Volume 308, Issue 1, pp 31–38 | Cite as

Immunoadsorber for specific apheresis of autoantibodies in the treatment of bullous pemphigoid

  • Michael MersmannEmail author
  • Jenny Dworschak
  • Kristin Ebermann
  • Lars Komorowski
  • Wolfgang Schlumberger
  • Winfried Stöcker
  • Detlef Zillikens
  • Christian Probst
  • Enno Schmidt
Original Paper


Bullous pemphigoid (BP) is an autoimmune blistering skin disease associated with autoantibodies against two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. As the pathogenic relevance of antibodies against the immunodominant NC16A domain of BP180 has been clearly demonstrated, specific removal of these antibodies should be a rational therapeutic approach. Here, we evaluated three recombinant forms of bacterially produced BP180 NC16A, a monomer, trimer, and tetramer, together with different matrices for their efficacy to specifically adsorb autoantibodies from BP plasma samples. An adsorber consisting of NC16A-trimer coupled to NHS-activated Sepharose 4 Fast Flow revealed satisfying adsorption rates and a high specificity. The NC16A-trimer adsorber was regenerable and autoclavable. It has the potential to be used for specific immunoadsorption to treat severe and refractory BP and other pemphigoid diseases associated with BP180 NC16A reactivity.


BP180 Immunoadsorption Autoantibodies Bullous pemphigoid 



This work was supported by the European Regional Development Fund (project no. 122-09-017) and the Excellence Cluster Inflammation@Interfaces (DFG EXC306/1 and 2).

Compliance with ethical standards

Conflict of interest

MM, KE, LK, CP are employees, WS and WSch are board members of EUROIMMUN AG. ES and DZ have a scientific cooperation with EUROIMMUN.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Michael Mersmann
    • 1
    Email author
  • Jenny Dworschak
    • 2
  • Kristin Ebermann
    • 1
  • Lars Komorowski
    • 1
  • Wolfgang Schlumberger
    • 1
  • Winfried Stöcker
    • 1
  • Detlef Zillikens
    • 3
  • Christian Probst
    • 1
  • Enno Schmidt
    • 2
    • 3
  1. 1.Institute of Experimental ImmunologyLübeckGermany
  2. 2.Lübeck Institute of Experimental DermatologyUniversity of LübeckLübeckGermany
  3. 3.Department of DermatologyUniversity of LübeckLübeckGermany

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