Archives of Dermatological Research

, Volume 305, Issue 9, pp 777–786 | Cite as

The effect of ultraviolet radiation on the transforming growth factor beta 1/Smads pathway and p53 in actinic keratosis and normal skin

  • Dan Xu
  • Ruihong Yuan
  • Hua Gu
  • Tongyun Liu
  • Ying Tu
  • Zhi Yang
  • Li He
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

Ultraviolet (UV) radiation is considered to be essential for the progression of actinic keratosis (AK) to squamous cell carcinoma (SCC); however, the mechanisms have not been fully elucidated. To understand this process, the effects of UV radiation on the transforming growth factor beta 1 (TGFβ1)/Smads pathway and p53 in normal skin and AK were studied. Normal human skin and AK tissues were cultured and divided into the following four groups according to the UV radiation dose: 0 (control group), 5, 10, and 20 J/cm2. The tissues were radiated for four consecutive days; 24 h after radiation, the tissues were collected for investigation. Compared with the control group, greater proliferative inhibition and apoptosis were induced by UV radiation in normal skin than AK. The expression of TGFβ1, Smad7, and p53 was increased in AK and normal skin, while the level of TβRII was decreased. Smad2 was reduced in AK only. The expressions of TβRI, Smad3, and Smad4 were not significantly changed. The results demonstrated that although p53 was induced, suppression of the TGFβ1/Smads pathway by UV radiation might contribute to the progression of AK to SCC.

Keywords

TGFβ1 Smad p53 Actinic keratosis Ultraviolet radiation 

Abbreviations

AK

Actinic keratosis

SCC

Squamous cell carcinoma

TGFβ1

Transforming growth factor beta 1

TβRI

Type I TGFβ receptor

TβRII

Type II TGFβ receptor

UV

Ultraviolet

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Notes

Acknowledgments

This work was supported by a grant from the National Natural Science Foundation of China (No.: 30860257). The paper was kindly revised by Hai-Jing Yu, PhD.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Dan Xu
    • 1
    • 2
  • Ruihong Yuan
    • 1
  • Hua Gu
    • 1
  • Tongyun Liu
    • 1
  • Ying Tu
    • 1
  • Zhi Yang
    • 1
  • Li He
    • 1
  1. 1.Department of Dermatology, The First Affiliated Hospital of Kunming Medical UniversityResearch Center of Dermatology of Yunnan ProvinceKunmingChina
  2. 2.College of Basic MedicineKunming Medical UniversityKunmingChina

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