Archives of Dermatological Research

, Volume 305, Issue 6, pp 535–544 | Cite as

Effects of a sunscreen formulation on albino hairless mice: a morphological approach

  • Bryan Hudson Hossy
  • Alvaro Augusto da Costa Leitão
  • Flávio Barbosa Luz
  • Elisabete Pereira dos Santos
  • Silvana Allodi
  • Marcelo de Pádula
  • Nádia Campos de Oliveira Miguel
Original Paper

Abstract

The purpose of the study was to evaluate the effects of a sunscreen formulation on the skin of albino hairless mice subjected to simulated solar light (SSL) in terms of morphological changes. Young adult albino hairless mice HRS/J (n = 36) were used as an experimental model for determining skin photoaging changes. Mice were irradiated with SSL, and the sunscreen (estimated SPF 30, PF-UVA) was obtained from the Pharmacy College/UFRJ, Brazil. The animals were divided into four groups: non-treated (G1), radiation only (G2), sunscreen-treated (G3) and vehicle + radiation (G4). Animals from groups G2, G3 and G4 were irradiated weekly (5 weeks), with no immobilization. One week after the final exposure, the dorsal skin was observed using a dermatoscopic camera. Biopsies were analyzed in order to quantify neovascularization and to evaluate histological aspects of the skin. Neovascularization was also evaluated with immunohistochemical reactions for the Von Willebrand factor. Animals from G2 displayed classical morphological changes denoting skin photoaging: thickening of the epidermis, increased dermal cellularity, follicular keratosis, sebaceous gland hyperplasia, and angiogenesis. Animals from groups G3 and G1 displayed similar morphological profiles, without these changes. Animals from group G4 showed more morphological changes than group G2, emphasizing the relative importance of the putative photosensitizing components present in the vehicle formulation. The extent of the morphological skin changes suggested that the sunscreen formulation was effective against SSL, and showed the importance of assessing the phototoxicity of vehicle formulations.

Keywords

Skin photoaging Sun exposure Sunlight simulator Ultraviolet radiation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Bryan Hudson Hossy
    • 1
  • Alvaro Augusto da Costa Leitão
    • 2
  • Flávio Barbosa Luz
    • 3
  • Elisabete Pereira dos Santos
    • 4
  • Silvana Allodi
    • 5
  • Marcelo de Pádula
    • 1
    • 6
  • Nádia Campos de Oliveira Miguel
    • 7
  1. 1.Programa de Pós Graduação em Ciências Farmacêuticas, Faculdade de FarmáciaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Laboratório de Radiobiologia Molecular, Instituto de Biofísica Carlos Chagas FilhoUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  3. 3.Departamento de Clínica Médica/Dermatologia, Faculdade de MedicinaUniversidade Federal FluminenseRio de JaneiroBrazil
  4. 4.Departamento de Medicamentos, Faculdade de FarmáciaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  5. 5.Programa de Neurobiologia, Instituto de Biofísica Carlos Chagas FilhoUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  6. 6.Laboratório de Diagnóstico Laboratorial e Hematologia, Programa de Pós Graduação em Ciências Farmacêuticas, Faculdade de FarmáciaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  7. 7.Programa de Biologia Celular e do Desenvolvimento, Instituto de Ciências BiomédicasUFRJRio de JaneiroBrazil

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