Role of cellular oxidative stress and cytochrome c in the pathogenesis of psoriasis
- 596 Downloads
Oxidative-free radicals and apoptosis have linked to chronic skin diseases. Higher levels of oxidative radicals and the release of mitochondrial cytochrome c may have a role in the pathogenesis of psoriasis. We investigated the possible role of cellular oxidative stress and release of cytochrome c of mitochondria in the pathogenesis of psoriasis. Disease severity was assessed by psoriasis area severity index score (PASI) of 55 psoriasis patients, they grouped as mild (11), moderate (20) and severe (24), also 20 healthy individuals used as controls. All groups were subjected for serum malondialdehyde (MDA), nitric oxide (NO·), superoxide dismutase (SOD), catalase (CAT), total antioxidant status (TAS) and serum cytochrome c concentrations. We found that, (1) Severity wise increase in MDA and NO·, and decrease in SOD, CAT and TAS levels in all patients with different degrees of psoriasis; (2) PASI showed positive correlation with the increase in MDA and NO·, and negatively with decreased SOD, CAT and TAS levels; (3) significant increase in cytochrome c level was observed among psoriasis patients which showed negative correlation to MDA and NO· levels in mild and positively with moderate and severe groups. The release of mitochondrial cytochrome c indicates the induction of apoptosis mediated via oxidative stress which ultimately plays role in the pathogenesis of psoriasis.
KeywordsMitochondria Psoriasis PASI score Apoptosis cytochrome c Oxidative stress Antioxidant status Antioxidant enzymes
Conflict of interest
The authors declare that they have no conflict of interest.
- 20.Karaarslan IK, Girgin SF, Ertam I, Alper S, Ozturk G, Yildirim SE (2006) Broad-band ultraviolet B phototherapy is associated with elevated serum thiobarbituric acid reactive substance and nitrite-nitrate levels in psoriatic patients. J Eur Acad Dermatol Venereol 20(10):1226–1231CrossRefGoogle Scholar
- 30.Mcgill A, Frank A, Emmett N, Turnbull DM, Birch MA, Reynolds NJ (2005) The anti-psoriatic drug anthralin accumulates in keratinocyte mitochondria, dissipates mitochondrial membrane potential, and induces apoptosis through a pathway dependent on respiratory competent mitochondria. FASEB J 19(8):1012–1014PubMedGoogle Scholar
- 40.Sikar AA, Ozdoğan HK, Bayramgürler D, Cekmen MB, Bilen N, Kıran R (2011) Nitric oxide and malondialdehyde levels in plasma and tissue of psoriasis patients. J Eur Acad Dermatol Venereol 1468–3083Google Scholar
- 42.Sravani PV, Babu NK, Gopal KV, Rao GR, Rao AR, Moorthy B, Rao TR (2009) Determination of oxidative stress in vitiligo by measuring superoxide dismutase and catalase levels in vitiliginous and non-vitiliginous skin. Indian J Dermatol Venereol Leprol 3:268–271Google Scholar
- 50.Wenhua G, Yongmei P, Kathy QL, Donald CC (2001) Temporal relationship between cytochrome c release and mitochondrial swelling during UV-induced apoptosis in living HeLa cells. J Cell Sci 114:2855–2862Google Scholar