Hyaluronan minimizes effects of UV irradiation on human keratinocytes

  • Martina Hašová
  • Tomáš Crhák
  • Barbora Šafránková
  • Jana Dvořáková
  • Tomáš Muthný
  • Vladimír Velebný
  • Lukáš Kubala
Original Paper


Exposure to ultraviolet (UV) irradiation has detrimental effects on skin accompanied by the increased metabolism of hyaluronan (HA), a linear polysaccharide important for the normal physiological functions of skin. In this study, the modulation of human keratinocyte response to UVB irradiation by HA (970 kDa) was investigated. Immortalized human keratinocytes (HaCaT) were irradiated by a single dose of UVB and immediately treated with HA for 6 and 24 h. The irradiation induced a significant decrease in the gene expression of CD44 and toll-like receptor 2 6 h after irradiation. The expressions of other HA receptors, including toll-like receptor 4 and the receptor for HA-mediated motility, were not detected in either the control or UVB-irradiated or HA-treated HaCaT cells. UVB irradiation induced a significant decrease in the gene expression of HA synthase-2 and hyaluronidase-2 6 h after irradiation. The expressions of HA synthase-3 and hyaluronidase-3 were not significantly modulated by UV irradiation. Interestingly, HA treatment did not significantly modulate any of these effects. In contrast, HA significantly suppressed UVB-induced pro-inflammatory cytokine release including interleukin-6 and interleukin-8. Similarly, HA treatment reduced the UVB-mediated production of transforming growth factor β1. HA treatment also significantly reduced the UV irradiation-mediated release of soluble CD44 into the media. Finally, HA partially, but significantly, suppressed the UVB-induced decrease in cell viability. Data indicate that HA had significant protective effects for HaCaT cells against UVB irradiation.


Hyaluronan Inflammation Keratinocyte Ultraviolet light 



The study was partly supported by grant No. 305/08/1704 from the Czech Science Foundation and research plans AV0Z50040507 and AV0Z50040702. The authors would like to thank J. Stejskalova, K. Becickova and M. Felgrova for their technical assistance with cell cultivation and protein assays.

Conflict of interest

Drs. Hasova, Dvorakova, and Muthny are Contipro Group employees; Dr. Velebny is a Contipro Group employee and stockholder.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Martina Hašová
    • 1
    • 2
  • Tomáš Crhák
    • 3
  • Barbora Šafránková
    • 3
  • Jana Dvořáková
    • 1
  • Tomáš Muthný
    • 1
  • Vladimír Velebný
    • 1
  • Lukáš Kubala
    • 3
  1. 1.CPN s.r.o, Dolni DobroucDolni DoboucCzech Republic
  2. 2.Department of Physiology and Immunology of Animals, Institute of Experimental Biology, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  3. 3.Institute of BiophysicsAcademy of Sciences of the Czech RepublicBrnoCzech Republic

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