Hyaluronan minimizes effects of UV irradiation on human keratinocytes
- 581 Downloads
Exposure to ultraviolet (UV) irradiation has detrimental effects on skin accompanied by the increased metabolism of hyaluronan (HA), a linear polysaccharide important for the normal physiological functions of skin. In this study, the modulation of human keratinocyte response to UVB irradiation by HA (970 kDa) was investigated. Immortalized human keratinocytes (HaCaT) were irradiated by a single dose of UVB and immediately treated with HA for 6 and 24 h. The irradiation induced a significant decrease in the gene expression of CD44 and toll-like receptor 2 6 h after irradiation. The expressions of other HA receptors, including toll-like receptor 4 and the receptor for HA-mediated motility, were not detected in either the control or UVB-irradiated or HA-treated HaCaT cells. UVB irradiation induced a significant decrease in the gene expression of HA synthase-2 and hyaluronidase-2 6 h after irradiation. The expressions of HA synthase-3 and hyaluronidase-3 were not significantly modulated by UV irradiation. Interestingly, HA treatment did not significantly modulate any of these effects. In contrast, HA significantly suppressed UVB-induced pro-inflammatory cytokine release including interleukin-6 and interleukin-8. Similarly, HA treatment reduced the UVB-mediated production of transforming growth factor β1. HA treatment also significantly reduced the UV irradiation-mediated release of soluble CD44 into the media. Finally, HA partially, but significantly, suppressed the UVB-induced decrease in cell viability. Data indicate that HA had significant protective effects for HaCaT cells against UVB irradiation.
KeywordsHyaluronan Inflammation Keratinocyte Ultraviolet light
The study was partly supported by grant No. 305/08/1704 from the Czech Science Foundation and research plans AV0Z50040507 and AV0Z50040702. The authors would like to thank J. Stejskalova, K. Becickova and M. Felgrova for their technical assistance with cell cultivation and protein assays.
Conflict of interest
Drs. Hasova, Dvorakova, and Muthny are Contipro Group employees; Dr. Velebny is a Contipro Group employee and stockholder.
- 6.Dai G, Freudenberger T, Zipper P, Melchior A, Grether-Beck S, Rabausch B, de Groot J, Twarock S, Hanenberg H, Homey B, Krutmann J, Reifenberger J, Fischer JW (2007) Chronic ultraviolet B irradiation causes loss of hyaluronic acid from mouse dermis because of down-regulation of hyaluronic acid synthases. Am J Pathol 171(5):1451–1461PubMedCrossRefGoogle Scholar
- 7.Debacq-Chainiaux F, Borlon C, Pascal T, Royer V, Eliaers F, Ninane N, Carrard G, Friguet B, de Longueville F, Boffe S, Remacle J, Toussaint O (2005) Repeated exposure of human skin fibroblasts to UVB at subcytotoxic level triggers premature senescence through the TGF-beta1 signaling pathway. J Cell Sci 118(Pt 4):743–758PubMedCrossRefGoogle Scholar
- 12.Kolarova H, Bino L, Pejchalova K, Kubala L (2010) The expression of NADPH oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line a549. Folia Biol (Praha) 56(5):211–217Google Scholar
- 21.Safrankova B, Gajdova S, Kubala L (2010) The potency of hyaluronan of different molecular weights in the stimulation of blood phagocytes. Med Inflam, article ID 380948. doi: 10.1155/2010/380948
- 23.Sudel KM, Venzke K, Mielke H, Breitenbach U, Mundt C, Jaspers S, Koop U, Sauermann K, Knussman-Hartig E, Moll I, Gercken G, Young AR, Stab F, Wenck H, Gallinat S (2005) Novel aspects of intrinsic and extrinsic aging of human skin: beneficial effects of soy extract. Photochem Photobiol 81(3):581–587PubMedCrossRefGoogle Scholar
- 26.Tzellos TG, Klagas I, Vahtsevanos K, Triaridis S, Printza A, Kyrgidis A, Karakiulakis G, Zouboulis CC, Papakonstantinou E (2009) Extrinsic ageing in the human skin is associated with alterations in the expression of hyaluronic acid and its metabolizing enzymes. Exp Dermatol 18(12):1028–1035PubMedCrossRefGoogle Scholar