A multi-component herbal preparation (PADMA 28) improves structure/function of corticosteroid-treated skin, leading to improved wound healing of subsequently induced abrasion wounds in rats
- 190 Downloads
PADMA 28 is a multi-component herbal mixture formulated according to an ancient Tibetan recipe. PADMA 28 is known to stimulate collagen production and reduced levels of collagen-degrading matrix metalloproteinases (MMPs). The goal of the present study was to determine whether topical treatment of rat skin with PADMA 28 would improve skin structure/function, and whether subsequently induced abrasion wounds would heal more rapidly in skin that had been pretreated with PADMA 28. Hairless rats were exposed to a potent topical corticosteroid (Temovate) in combination with either DMSO alone or with PADMA 28 given topically. At the end of the treatment period, superficial wounds were created in the skin, and time to wound closure was assessed. Collagen production and matrix-degrading MMPs were assessed. Abrasion wounds in skin that had been pretreated with PADMA 28 healed more rapidly than did wounds in Temovate plus DMSO-treated skin. Under conditions in which improved wound healing was observed, there was an increased collagen production and decreased MMP expression, but no significant epidermal hyperplasia and no evidence of skin irritation. The ability to stimulate collagen production and inhibit collagen-degrading enzymes in skin and facilitate more rapid wound closure without irritation should provide a rationale for development of the herbal preparation as a “skin-repair” agent.
KeywordsType I collagen Matrix metalloproteinase Wound healing Skin repair PADMA 28 Temovate
The authors would like to acknowledge PADMA AG (Schwerzenbach, Switzerland) as the source of the PADMA 28 used in the study. The authors would also like to thank Lisa Riggs and Ron Craig (Histomorphometry Core Laboratory) for help with histology. The core laboratory is supported by the Department of Pathology, University of Michigan.
Conflict of interest statement
The authors state no competing interests.
- 12.Griffiths CE, Kang S, Ellis CN et al (1995) Two concentrations of topical tretinoin (retinoic acid) cause similar improvement of photoaging but different degrees of irritation. A double-blind, vehicle-controlled comparison of 0.1% and 0.025% tretinoin creams. Arch Dermatol 131:1037–1044CrossRefPubMedGoogle Scholar
- 13.rGya mtsho sDS (1994) Gsoba rig pa’ bsten bcos smen bla’ dgongs rgyen rgyud bzhi’ gsal byed be dur snogpo’ mallika zhes bya bu bzhugs so—Smed cha. (Commentary to the rgyud bzhi, The Blue Beryll, vol II). Tibetan Medical & Astro Institute, DharamsalaGoogle Scholar
- 15.Kang S, Duell EA, Fisher GJ et al (1995) Application of retinol to human skin in vivo induces epidermal hyperplasia and cellular retinoid-binding proteins characteristic of retinoic acid but without measurable retinoic acid levels or irritation. J Invest Dermatol 105:549–556CrossRefPubMedGoogle Scholar
- 17.Kligman AM, Balin AK (1989) Aging of human skin. In: Balin AK, Kligman AM (eds) Aging and the human skin. Raven Press, New York, pp 1–11Google Scholar
- 21.Lavker RM (1995) Cutaneous aging: chronologic versus photoaging. In: Gilchrest BA (ed) Photoaging. Blackwell, Cambridge, MA, pp 123–135Google Scholar
- 31.Sallon S, Beer G, Rosenfeld J et al (1998) The efficacy of PADMA 28, a herbal preparation, in the treatment of intermittent claudication: a controlled double-blind pilot study with objective assessment of chronic occlusive arterial disease patients. J Vasc Invest 4:129–136Google Scholar
- 32.Samochowiec L, Wojcicki J, Kosmider K et al (1987) Wirksamkeitsprüfung von PADMA 28 bei der Behandlung von Patienten mit chronischen arteriellen Durchblutungsstörungen. Herba Pol 33:29–41Google Scholar
- 38.Smulski HS, Wojcicki J (1995) Placebo controlled, double blind trial to determine the efficacy of the Tibetan plant preparation PADMA 28 for intermittent claudication. Altern Ther Health Med 1:44–49Google Scholar