Gingko biloba extract reduces VEGF and CXCL-8/IL-8 levels in keratinocytes with cumulative effect with epigallocatechin-3-gallate
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In skin inflammation, vascular endothelial growth factor (VEGF) and CXCL-8/IL-8 play an important role and are produced by activated keratinocytes. Extracts from Ginkgo biloba leaves (GBE), widely used in phytotherapy, have been reported to exert antioxidant and anti-inflammatory properties in the skin. We therefore evaluated the effects of GBE on the release of VEGF and CXCL8/IL-8 by normal human keratinocytes (NHKs) activated by tumor necrosis factor α (TNFα). Moreover, as we previously showed that epigallocatechin-3-gallate (EGCG) reduces VEGF and CXCL8/IL-8 secretion in TNFα-activated NHKs, we also tested its effect in association with GBE. Our results showed that GBE exerted a potent inhibition on VEGF and CXCL8/IL-8 levels in activated cells. In association with EGCG, GBE down-regulated VEGF and CXCL8/IL-8 levels in a cumulative manner in TNFα-stimulated NHKs. These results suggest that GBE, alone or in association with EGCG may contribute to moderate inflammatory processes in skin diseases associated with angiogenesis.
KeywordsGinkgo biloba Normal human keratinocyte VEGF CXCL8/IL-8 Inflammation EGCG
Bovine pituitary extract
Epidermal growth factor
Enzyme-linked immunosorbent assays
Ginkgo biloba extract
3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide
Normal human keratinocyte
Tumor necrosis factor α
We thank pediatric service (Tbis) of Edouard Herriot Hospital in Lyon for their cooperation in collecting skin biopsy samples.
Conflict of interest statement
The authors declare that they have no conflict of interest.