Archives of Dermatological Research

, Volume 301, Issue 10, pp 731–737 | Cite as

Circulatory levels of antioxidants and lipid peroxidation in Indian patients with generalized and localized vitiligo

  • Rehan Khan
  • Abhigyan Satyam
  • Somesh Gupta
  • Vinod K. Sharma
  • Alpana SharmaEmail author
Original Paper


Vitiligo is an acquired skin disease, characterized by white areas on the skin due to loss of functional melanocytes. The pathogenesis of the disease is still unclear. Published data show the involvement of oxidative stress in the pathophysiology of vitiligo. A total of 30 vitiligo patients and 30 healthy controls were included in this study. We estimated serum levels of malondialdehyde (MDA), vitamins E and C, total antioxidant activity and whole blood levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in vitiligo patients and controls. We found significantly higher levels of MDA and significantly lower levels of SOD, GPx, vitamins C and E and total antioxidant activity in vitiligo patients compared with controls. This study is a maiden attempt to report on antioxidant parameters of both generalized/localized-type Indian vitiligo patients. Our results confirmed that oxidative stress may play an important role in the pathogenesis of vitiligo and cause melanocyte damage in vitiligo.


Vitiligo Oxidative stress SOD MDA GPx AOA 



This study was supported by the All India Institute of Medical Sciences, Institute Research Grant.


  1. 1.
    Agrawal D, Shajil EM, Marfatia YS, Begum R (2004) Study on the antioxidant status of vitiligo patients of different age groups in Baroda. Pigment Cell Res 17:289–294. doi: 10.1111/j.1600-0749.2004.00149.x PubMedCrossRefGoogle Scholar
  2. 2.
    Arican O, Kurutas EB (2008) Oxidative stress in the blood of patients with active localized vitiligo. Acta Dermatovenerol Alp Panonica Adr 17(1):12–16Google Scholar
  3. 3.
    Beazley WD, Gaze D, Panske A et al (1999) Serum selenium levels and blood glutathione peroxidase activities in vitiligo. Br J Dermatol 141:301–303. doi: 10.1046/j.1365-2133.1999.02980.x PubMedCrossRefGoogle Scholar
  4. 4.
    Bhatia PS, Mohan L, Pandley N, Singh KK, Arora SK, Mukhija RD (1992) Genetic nature of vitiligo. J Dermatol Sci 4:180–184. doi: 10.1016/0923-1811(92)90017-6 PubMedCrossRefGoogle Scholar
  5. 5.
    Boisseau-Garsaud AM, Garsaud P, Lejoly-Boisseau H et al (2002) Increase in total blood antioxidant status and selenium levels in black patients with active vitiligo. Int J Dermatol 41:640–642. doi: 10.1046/j.1365-4362.2002.01472.x PubMedCrossRefGoogle Scholar
  6. 6.
    Chakraborty DP, Roy S, Chakraborty AK (1996) Vitiligo, psoralen, and melanogenesis: some observations and understanding. Pigment Cell Res 9:107–116. doi: 10.1111/j.1600-0749.1996.tb00098.x PubMedCrossRefGoogle Scholar
  7. 7.
    Chu CY, Liu YL, Chiu HC, Jee SH (2006) Dopamine-induced apoptosis in human melanocytes involves generation of reactive oxygen species. Br J Dermatol 154:1071–1079. doi: 10.1111/j.1365-2133.2006.07293.x PubMedCrossRefGoogle Scholar
  8. 8.
    Dell’anna ML, Maresca V, Briganti S et al (2001) Mitochondrial impairment in peripheral blood mononuclear cells during the active phase of vitiligo. J Invest Dermatol 117:908–913. doi: 10.1046/j.0022-202x.2001.01459.x PubMedCrossRefGoogle Scholar
  9. 9.
    Dell’anna ML, Urbanelli S, Mastrofrancesco A et al (2003) Alterations of mitochondria in peripheral blood mononuclear cells of vitiligo patients. Pigment Cell Res 16:553–559. doi: 10.1034/j.1600-0749.2003.00087.x PubMedCrossRefGoogle Scholar
  10. 10.
    Draper HH, Hadley M (1990) Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 186:421–431. doi: 10.1016/0076-6879(90)86135-I PubMedCrossRefGoogle Scholar
  11. 11.
    Em S, Laddha NC, Chatterjee S, Gani AR, Malek RA, Shah BJ, Begum R (2007) Association of catalase T/C exon 9 and glutathione peroxidase codon 200 polymorphisms in relation to their activities and oxidative stress with vitiligo susceptibility in Gujarat population. Pigment Cell Res 20:405–407PubMedGoogle Scholar
  12. 12.
    Gavalas NG, Akhtar S, Gawkrodger DJ, Watson PF, Weetman AP, Kemp EH (2006) Analysis of allelic variants in the catalase gene in patients with the skin depigmenting disorder vitiligo. Biochem Biophys Res Commun 345:1586–1591. doi: 10.1016/j.bbrc.2006.05.063 PubMedCrossRefGoogle Scholar
  13. 13.
    Hasse S, Gibbons NCJ, Rokos H et al (2004) Perturbed 6 tetrahydrobopterin recycling via decreased dihydropteridine reductase in vitiligo: more evidence for H2O2 stress. J Invest Dermatol 122:307–313. doi: 10.1046/j.0022-202X.2004.22230.x PubMedCrossRefGoogle Scholar
  14. 14.
    Hazneci E, Karabulut AB, Ozturk C et al (2005) A comparative study of superoxide dismutase, catalase, and glutathione peroxidase activities and nitrate levels in vitiligo patients. Int J Dermatol 44:636–640. doi: 10.1111/j.1365-4632.2004.02027.x PubMedCrossRefGoogle Scholar
  15. 15.
    Jimbow K, Chen H, Park JS et al (2001) Increased sensitivity of melanocytes to oxidative stress and abnormal expression of tyrosinase-related protein in vitiligo. Br J Dermatol 144:55–65. doi: 10.1046/j.1365-2133.2001.03952.x PubMedCrossRefGoogle Scholar
  16. 16.
    Koca R, Armutcu F, Altinyazar HC, Gurel A (2004) Oxidant antioxidant enzymes and lipid peroxidation in generalized vitiligo. Clin Exp Dermatol 29:406–409. doi: 10.1111/j.1365-2230.2004.01524.x PubMedCrossRefGoogle Scholar
  17. 17.
    Koga M, Tango T (1988) Clinical features and course of type A and type B vitiligo. Br J Dermatol 118:223–228. doi: 10.1111/j.1365-2133.1988.tb01778.x PubMedCrossRefGoogle Scholar
  18. 18.
    Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V (2001) Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 54:356–361PubMedCrossRefGoogle Scholar
  19. 19.
    Maccarrone M, Catani M, Iraci S et al (1997) A survey of reactive oxygen species and their role in dermatology. J Eur Acad Dermatol Venereol 8:185–202. doi: 10.1111/j.1468-3083.1997.tb00478.x CrossRefGoogle Scholar
  20. 20.
    Maresca V, Roccella M, Roccella F et al (1997) Increased sensitivity to peroxidative agents as a possible pathogenic factor of melanocyte damage in vitiligo. J Invest Dermatol 109:310–313. doi: 10.1111/1523-1747.ep12335801 PubMedCrossRefGoogle Scholar
  21. 21.
    McCormick DB, Green HL (1999) Vitamins. In: Carl A, Burtis CA, Edward R, Ashwood ER (eds) Tietz textbook of clinical chemistry. W.B. Saunders, Delhi, pp 1024–1025Google Scholar
  22. 22.
    Paglia DE, Valentine WN (1967) Study on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169PubMedGoogle Scholar
  23. 23.
    Passi S, Grandinetti M, Maggio F et al (1998) Epidermal oxidative stress in vitiligo. Pigment Cell Res 11:81–85. doi: 10.1111/j.1600-0749.1998.tb00714.x PubMedCrossRefGoogle Scholar
  24. 24.
    Picardo M, Passi S, Morrone A et al (1994) Antioxidant status in the blood of patients with active vitiligo. Pigment Cell Res 7:110–115. doi: 10.1111/j.1600-0749.1994.tb00034.x PubMedCrossRefGoogle Scholar
  25. 25.
    Procter PH, Reynolds ES (1984) Free radicals and disease in man. Physiol Chem Phys Med NMR 16:175–195Google Scholar
  26. 26.
    Riley PA (1988) Radicals in melanin biochemistry. Ann N Y Acad Sci 55:111–120. doi: 10.1111/j.1749-6632.1988.tb22325.x CrossRefGoogle Scholar
  27. 27.
    Saha N, Ahmed MA, Wasfi AI, El Munshid HA (1982) Distribution of serum proteins, red cell enzymes and hemoglobin in vitiligo. Hum Hered 32:46–48. doi: 10.1159/000153257 PubMedCrossRefGoogle Scholar
  28. 28.
    Schallreuter KU, Bahadoran P, Picardo M et al (2008) Vitiligo pathogenesis: autoimmune disease, genetic defect, excessive reactive oxygen species, calcium imbalance, or what else? Exp Dermatol 17(2):139–140. doi: 10.1111/j.1600-0625.2007.00666_2.x discussion 141-60PubMedCrossRefGoogle Scholar
  29. 29.
    Schallrueter KU, Moore J, Wood JM et al (2001) Epidermal H2O2 accumulation alters tetrahydrobiopterin (6BH4) recycling in vitiligo: identification of a general mechanism in regulation of all 6BH4-dependent processes. J Invest Dermatol 116:167–174. doi: 10.1046/j.1523-1747.2001.00220.x CrossRefGoogle Scholar
  30. 30.
    Schallreuter KU, Moore J, Wood JM et al (1999) In vivo and in vitro evidence for hydrogen peroxide (H2O2) accumulation in the epidermis of patients with vitiligo and its successful removal by a UVB-activated pseudocatalase. J Investig Dermatol Symp Proc 4(1):91–96. doi: 10.1038/sj.jidsp.5640189 PubMedCrossRefGoogle Scholar
  31. 31.
    Schallreuter KU, Wood JM, Berger J (1991) Low catalase levels in the epidermis of patients with vitiligo. J Invest Dermatol 97(6):1081–1085. doi: 10.1111/1523-1747.ep12492612 PubMedCrossRefGoogle Scholar
  32. 32.
    Schallrueter KU, Wood JM, Ziegler I, Lemke KR, Pittelkow MR, Lindsay NJ, Gutlich M (1994) Defective tetrahydrobiopterin and catecholamine biosynthesis in the depigmentation disorder vitiligo. Biochim Biophys Acta 1226:181–192Google Scholar
  33. 33.
    Shajil EM, Begum R (2006) Antioxidant status of segmental and non-segmental vitiligo. Pigment Cell Res 19:179–180. doi: 10.1111/j.1600-0749.2006.00299.x PubMedCrossRefGoogle Scholar
  34. 34.
    Shajil EM, Chatterjee S, Agrawal D, Bagchi T, Begum R (2006) Vitiligo: pathomechanisms and genetic polymorphism of susceptible genes. Indian J Exp Biol 44(7):526–539PubMedGoogle Scholar
  35. 35.
    Shalbaf M, Gibbons NC, Wood JM, Maitland DJ, Rokos H, Elwary SM, Marles LK, Schallreuter KU (2008) Presence of epidermal allantoin further supports oxidative stress in vitiligo. Exp Dermatol 17:761–770. doi: 10.1111/j.1600-0625.2008.00697.x PubMedCrossRefGoogle Scholar
  36. 36.
    Strömberg S, Björklund MG, Asplund A, Rimini R, Lundeberg J, Nilsson P, Pontén F, Olsson MJ (2008) Transcriptional profiling of melanocytes from patients with vitiligo vulgaris. Pigment Cell Melanoma Res 21:162–171. doi: 10.1111/j.1755-148X.2007.00429.x PubMedCrossRefGoogle Scholar
  37. 37.
    Taïeb A, Morice-Picard F, Jouary T, Ezzedine K, Cario-André M, Gauthier Y (2008) Segmental vitiligo as the possible expression of cutaneous somatic mosaicism: implications for common non-segmental vitiligo. Pigment Cell Melanoma Res 21:646–652. doi: 10.1111/j.1755-148X.2008.00511.x PubMedCrossRefGoogle Scholar
  38. 38.
    Thomas CE, Morehouse LA, Aust SD (1985) Ferritin and superoxide-dependent lipid peroxidation. J Biol Chem 260(6):3275–3280PubMedGoogle Scholar
  39. 39.
    Tobin DJ, Swanson NN, Pittelkow MR, Peters EM, Schallreuter KU (2000) Melanocytes are not absent in lesional skin of long duration vitiligo. J Pathol 191(4):407–416. doi: 10.1002/1096-9896(2000)9999:9999<::AID-PATH659>3.0.CO;2-D PubMedCrossRefGoogle Scholar
  40. 40.
    Uhm YK, Yoon SH, Kang IJ, Chung JH, Yim SV, Lee MH (2007) Association of glutathione S-transferase gene polymorphisms (GSTM1 and GSTT1) of vitiligo in Korean population. Life Sci 81:223–227. doi: 10.1016/j.lfs.2007.05.006 PubMedCrossRefGoogle Scholar
  41. 41.
    Westerhof W, d’Ischia M (2007) Vitiligo puzzle: the pieces fall in place. Pigment Cell Res 20(5):345–359PubMedGoogle Scholar
  42. 42.
    Wood JM, Chavan B, Hafeez I, Schallreuter KU (2004) Regulation of tyrosinase by tetrahydropteridines and H2O2. Biochem Biophys Res Commun 325:1412–1417. doi: 10.1016/j.bbrc.2004.10.185 PubMedCrossRefGoogle Scholar
  43. 43.
    Yildirim M, Baysal V, Inaloz HS, Can M (2004) The role of oxidants and antioxidants in generalized vitiligo at the tissue level. J Eur Acad Dermatol Venereol 18:683–686. doi: 10.1111/j.1468-3083.2004.01080.x PubMedCrossRefGoogle Scholar
  44. 44.
    Yildirim M, Baysal V, Inaloz HS et al (2003) The role of oxidants and antioxidants in generalized vitiligo. J Dermatol 30:104–108PubMedGoogle Scholar
  45. 45.
    Yohn JJ, Norris DA, Yrastorza DG et al (1991) Disparate antioxidant enzyme activities in cultured human cutaneous fibroblasts, keratinocytes, and melanocytes. J Invest Dermatol 97:405–409. doi: 10.1111/1523-1747.ep12480983 PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Rehan Khan
    • 1
  • Abhigyan Satyam
    • 1
  • Somesh Gupta
    • 2
  • Vinod K. Sharma
    • 2
  • Alpana Sharma
    • 1
    Email author
  1. 1.Department of BiochemistryAll India Institute of Medical SciencesNew DelhiIndia
  2. 2.Department of Dermatology and VenereologyAll India Institute of Medical SciencesNew DelhiIndia

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