LPS induces melanogenesis through p38 MAPK activation in human melanocytes
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We have observed that lipopolysaccharide (LPS) induces pigmentation in melanocytes and in this study have examined whether these responses are mediated by the p38 mitogen-activated protein kinase (MAPK) signaling pathway. LPS appears to stimulate the pigmentation of melanocytes and cultured skin. LPS was found to induce the expression of microphthalmia-associated transcription factor (MITF) and tyrosinase protein in cells. Stimulation of melanocytes with LPS led to time dependent phosphorylation of p38 MAPK. Furthermore, p38 MAPK functionally regulated the LPS-induced melanin formation in melanocytes; a p38 MAPK inhibitor, SB203580, almost completely attenuated the LPS-mediated up-regulation of melanin synthesis and induction of MITF and tyrosinase expression. These findings indicate that activation of p38 MAPK plays an important role in LPS-induced melanogenesis by up-regulating MITF and tyrosinase expression.
KeywordsLipopolysaccharide (LPS) p38 mitogen-activated protein kinase Melanogenesis
This work was supported by the Korean Science and Engineering Foundation (KOSEF). Grant funded by the Korean government (MOST) (R13-2003-019) and the “GRRC” Project of Gyeonggi Provincial Government, Republic of Korea.
- 12.Moll I, Houdek P, Schmidt H, Moll R (1998) Characterization of epidermal wound healing in a human skin organ culture model: a human epidermal cell cultures: growth and differentiation in the absence of differentiation in the absence of dermal components or medium supplements acceleration by transplanted keratinocytes. J Invest Dermatol 111:251–258PubMedCrossRefGoogle Scholar
- 15.O’Neill LA (2002) Signal transduction pathways activated by the IL-1 receptor/Toll-like receptor superfamily. Curr Top Microbiol Immunol 270:247Google Scholar