Archives of Dermatological Research

, Volume 300, Issue 9, pp 531–535 | Cite as

Microencapsulated human hair dermal papilla cells: a substitute for dermal papilla?

  • Chang-min Lin
  • Yu Li
  • Ying-chang Ji
  • Huang Keng
  • Xiang-na Cai
  • Jin-kun Zhang
Short Communication


Dermal papillae (DP) play a pivotal role in hair formation, growth and cycling. However, the number of DP is limited. In this study, we report the production of “reconstructed DP” by enclosing DP cells within an alginate–polylysine–alginate (APA) semipermeable membrane. MTT assay and electron microscopy showed that the microencapsulated dermal papilla cells retained normal activity. The microcapsules were implanted into rat footpads, which lack follicles and sebaceous glands, to assess their inductive properties. Histologic examination showed that numbers of follicle and sebaceous gland structures formed in the footpads within 6–10-week period. At the 10 weeks following transplantation, hair fibers were visible in the footpad. These findings indicate that the DP cell microcapsules retain the capacity to initiate follicle regeneration and could be considered a substitute for fresh isolated DPs.


Dermal papilla Microcapsulation Hair follicle Reconstruction Transplantation 



Dermal papilla




[3-(4, 5-Dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide]



This research was supported by the National Natural Science Foundation of China (No.30170973; 30672184) and the Natural Science Foundation of Guangdong Province (No.010435; 06033496).


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Chang-min Lin
    • 1
  • Yu Li
    • 1
  • Ying-chang Ji
    • 1
  • Huang Keng
    • 2
  • Xiang-na Cai
    • 1
  • Jin-kun Zhang
    • 3
  1. 1.Department of Plastic and Reconstructive Surgery, Tissue Engineering, Laboratory, First Affiliated HospitalShantou University Medical CollegeShantou CityChina
  2. 2.Emergency Department, Second Affiliated HospitalShantou University Medical CollegeShantou CityChina
  3. 3.Department of Onco-Pathology and the Key Immunopathology Laboratory of Guangdong ProvinceShantou CityChina

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