Advertisement

Archives of Dermatological Research

, Volume 300, Issue 4, pp 161–166 | Cite as

Anti-tumor effect of PDT using Photofrin in a mouse angiosarcoma model

  • Iizuka Jin
  • Morimoto Yuji
  • Nakagishi Yoshinori
  • Kikuchi Makoto
  • Masuzawa Mikio
Original Paper

Abstract

Angiosarcoma, a malignant tumor of vascular endothelial cell origin, is a lethal disease for which complete cure is rarely seen. The objective of this study was to determine the efficiency of photodynamic therapy (PDT) as a new treatment for angiosarcoma. PDT (630 nm, 25 J/cm2) using Photofrin for a mouse angiosarcoma cell line of human origin (ISOS-1) showed that the rate of cell death increased with increase in the concentration of a photosensitizer (LD50: ∼2 μg/ml). Furthermore, PDT (630 nm, 100 J/cm2) with Photofrin (5 mg/kg, i.v.) in mice transplanted with ISOS-1 cells resulted in complete disappearance of the tumor in 40% of mice and marked inhibition of tumor growth in the remaining 60%. Significant increases in TUNEL-positive cells and Ki-67-positive cells ware seen 4 h after PDT, indicating that PDT led to not only cell death but also inhibition of the proliferation of angiosarcoma cells. The results show that PDT is effective for treatment of angiosarcoma.

Keywords

Photofrin Angiosarcoma Photodynamic therapy Mouse ISOS-1 cells 

Abbreviation

PDT

Photodynamic therapy

ROS

Reactive oxygen species

SCID

Severe combined immunodificiency

MTT

Methyl thiazolyl tetrazolium

PBS

Phosphate-buffered saline

HE

Hematoxylin-eosin

Notes

Acknowledgments

The authors thank Ms.Tatsuko Suga for performing histological studies.

References

  1. 1.
    Arbiser JL, Moses MA, Fernandez CA, Ghiso N, Cao Y, Klauber N, Frank D, Brownlee M, Flynn E, Parangi S, Byers HR, Folkman J (1997) Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc Natl Acad Sci USA 94:861–866PubMedCrossRefGoogle Scholar
  2. 2.
    Bernstein ZP, Wilson BD, Oseroff AR, Jones CM, Dozier SE, Brooks JS, Cheney R, Foulke L, Mang TS, Bellnier DA, Dougherty TJ (1999) Photofrin photodynamic therapy for treatment of AIDS-related cutaneous Kaposi’s sarcoma. AIDS 13:1697–1704PubMedCrossRefGoogle Scholar
  3. 3.
    Cortese DA, Edell ES, Kinsey JH (1997) Photodynamic therapy for early stage squamous cell carcinoma of the lung. Mayo Clin Proc 72:595–602PubMedGoogle Scholar
  4. 4.
    Engbrecht BW, Menon C, Kachur AV, Hahn SM, Fraker DL (1999) Photofrin-mediated photodynamic therapy induces vascular occlusion and apoptosis in a human sarcoma xenograft model. Cancer Res 59:4334–4342PubMedGoogle Scholar
  5. 5.
    Fata F, O’Reilly E, Ilson D, Pfister D, Leffel D, Kelsen DP, Schwartz GK, Casper ES (1999) Paclitaxel in the treatment of patients with angiosarcoma of the scalp or face. Cancer 86:2034–2037PubMedCrossRefGoogle Scholar
  6. 6.
    Krump-Konvalinkova V, Bittinger F, Olert J, Brauninger W, Brunner J, Kirkpatrick CJ (2003) Establishment and characterization of an angiosarcoma-derived cell line, AS-M. Endothelium 10:319–328PubMedCrossRefGoogle Scholar
  7. 7.
    Leunig A, Staub F, Peters J, Heimann A, Csapo C, Kempski O, Goetz AE (1994) Relation of early Photofrin uptake to photodynamically induced phototoxicity and changes of cell volume in different cell lines. Eur J Cancer 30A:78–83PubMedCrossRefGoogle Scholar
  8. 8.
    Lou PJ, Jones L, Hopper C (2003) Clinical outcomes of photodynamic therapy for head-and-neck cancer. Technol Cancer Res Treat 2:311–317PubMedGoogle Scholar
  9. 9.
    Mark RJ, Poen JC, Tran LM, Fu YS, Juillard GF (1996) Angiosarcoma. A report of 67 patients and a review of the literature. Cancer 77:2400–2406PubMedCrossRefGoogle Scholar
  10. 10.
    Mark RJ, Tran LM, Sercarz J, Fu YS, Calcaterra TC, Juillard GF (1993) Angiosarcoma of the head and neck. The UCLA experience 1955 through 1990. Arch Otolaryngol Head Neck Surg 119:973–978PubMedGoogle Scholar
  11. 11.
    Masuzawa M, Fujimura T, Tsubokawa M, Nishiyama S, Katsuoka K, Terada E, Kunita S, Sakurai Y, Kato H (1998) Establishment of a new murine-phenotypic angiosarcoma cell line (ISOS-1). J Dermatol Sci 16:91–98PubMedCrossRefGoogle Scholar
  12. 12.
    Middelkamp-Hup MA, Sanchez-Carpintero I, Kossodo S, Waterman P, Gonzalez S, Mihm MC Jr, Anderson RR (2003) Photodynamic therapy for cutaneous proliferative vascular tumors in a mouse model. J Invest Dermatol 121:634–639PubMedCrossRefGoogle Scholar
  13. 13.
    Ohguri T, Imada H, Nomoto S, Yahara K, Hisaoka M, Hashimoto H, Tokura Y, Nakamura K, Shioyama Y, Honda H, Terashima H, Moroi Y, Furue M, Korogi Y (2005) Angiosarcoma of the scalp treated with curative radiotherapy plus recombinant interleukin-2 immunotherapy. Int J Radiat Oncol Biol Phys 61:1446–1453PubMedGoogle Scholar
  14. 14.
    Overholt BF, Panjehpour M, Haydek JM (1999) Photodynamic therapy for Barrett’s esophagus: follow-up in 100 patients. Gastrointest Endosc 49:1–7PubMedCrossRefGoogle Scholar
  15. 15.
    Pawlik TM, Paulino AF, McGinn CJ, Baker LH, Cohen DS, Morris JS, Rees R, Sondak VK (2003) Cutaneous angiosarcoma of the scalp: a multidisciplinary approach. Cancer 98:1716–1726PubMedCrossRefGoogle Scholar
  16. 16.
    Peng Q, Warloe T, Berg K, Moan J, Kongshaug M, Giercksky KE, Nesland JM (1997) 5-Aminolevulinic acid-based photodynamic therapy. Clinical research and future challenges. Cancer 79:2282–2308PubMedCrossRefGoogle Scholar
  17. 17.
    Sasaki R, Soejima T, Kishi K, Imajo Y, Hirota S, Kamikonya N, Murakami M, Kawabe T, Ejima Y, Matsumoto A, Sugimura K (2002) Angiosarcoma treated with radiotherapy: impact of tumor type and size on outcome. Int J Radiat Oncol Biol Phys 52:1032–1040PubMedGoogle Scholar
  18. 18.
    Sitnik TM, Henderson BW (1998) The effect of fluence rate on tumor and normal tissue responses to photodynamic therapy. Photochem Photobiol 67:462–466PubMedCrossRefGoogle Scholar
  19. 19.
    Takeuchi Y, Kurohane K, Ichikawa K, Yonezawa S, Nango M, Oku N (2003) Induction of intensive tumor suppression by antiangiogenic photodynamic therapy using polycation-modified liposomal photosensitizer. Cancer 97:2027–2034PubMedCrossRefGoogle Scholar
  20. 20.
    Ulrich L, Krause M, Brachmann A, Franke I, Gollnick H (2000) Successful treatment of angiosarcoma of the scalp by intralesional cytokine therapy and surface irradiation. J Eur Acad Dermatol Venereol 14:412–415PubMedCrossRefGoogle Scholar
  21. 21.
    Ward JR, Feigenberg SJ, Mendenhall NP, Marcus RB Jr, Mendenhall WM (2003) Radiation therapy for angiosarcoma. Head Neck 25:873–878PubMedCrossRefGoogle Scholar
  22. 22.
    Whitehurst C, Pantelides ML, Moore JV, King TA, Blacklock NJ (1990) In vivo to post-mortem change in tissue penetration of red light. Lasers Med Sci 5:395–398CrossRefGoogle Scholar
  23. 23.
    Wollina U, Fuller J, Graefe T, Kaatz M, Lopatta E (2001) Angiosarcoma of the scalp: treatment with liposomal doxorubicin and radiotherapy. J Cancer Res Clin Oncol 127:396–399PubMedCrossRefGoogle Scholar
  24. 24.
    Xiao Z, Brown K, Tulip J, Moore RB (2003) Whole bladder photodynamic therapy for orthotopic superficial bladder cancer in rats: a study of intravenous and intravesical administration of photosensitizers. J Urol 169:352–356PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Iizuka Jin
    • 1
    • 2
  • Morimoto Yuji
    • 2
  • Nakagishi Yoshinori
    • 3
  • Kikuchi Makoto
    • 3
  • Masuzawa Mikio
    • 4
  1. 1.Department of DermatologyJapan Self Defense Force Central HospitalSetagayaJapan
  2. 2.Department of Integrative Physiology and Bio-Nano MedicineNational Defense Medical CollegeTokorozawaJapan
  3. 3.Department of Medical EngineeringNational Defense Medical CollegeTokorozawaJapan
  4. 4.Department of Dermatology, School of MedicineKitasato UniversitySagamiharaJapan

Personalised recommendations