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Archives of Dermatological Research

, Volume 300, Supplement 1, pp 69–76 | Cite as

Mast cell tryptase and photoaging: possible involvement in the degradation of extra cellular matrix and basement membrane proteins

  • Arunasiri IddamalgodaEmail author
  • Quang Trong Le
  • Kenichi Ito
  • Kiyotaka Tanaka
  • Hiroyuki Kojima
  • Hiroshi Kido
Original Paper

Abstract

Mast cells are widely distributed in the connective tissue of the body, but are particularly prominent in tissues such as skin. An increased number of mast cells can be found in the dermis under inflammatory conditions and ultraviolet (UV) exposed skin. Previous investigations have identified matrix metalloproteinases (MMPs) as key enzymes in the degradation of extra cellular matrix (ECM). This study reports about the potential contribution of human mast cell tryptase as a new triggering enzyme in matrix degradation process. Recent studies suggest that mast cell-derived proteases can activate MMPs. We investigated both the degradation of cellular matrix components and activation of MMPs by human tryptase. Mast cells are increased in photoaged skin and the increase of mast cell tryptase in UV irradiated skin was confirmed. Human mast cell tryptase was purified from human tonsils by a series of standard chromatographic procedures. Degradation of collagen type I was achieved by incubation of human type I collagen with tryptase and the fragments were quantified by SDS-PAGE and staining with Coomassie Brilliant Blue 250-R (CBB). Treatment with tryptase resulted in the activation of proMMP-9 as revealed by gelatinolytic activity in type IV collagen zymography. When tryptase was incubated with human type IV collagen, gradual degradation of intact collagen was detected by Western blotting. Furthermore, type IV collagen degradation was observed in the basement membrane (BM) of a three-dimensional (3D) skin model. Degranulation of mast cells, which release tryptase, can activate MMPs and causes direct damage to ECM proteins. These findings strongly implicate that tryptase either alone or in conjunction with activation of MMPs, can participate in ECM damage and the possible destruction of BM leading to photoaging.

Keywords

Tryptase Collagen MMP Mast cells Photoaging 

Abbreviations

UV

Ultraviolet

MMPs

Metalloproteinases

ECM

Extra cellular matrix

CBB

Coomassie brilliant blue 250-R

BM

Basement membrane

Notes

Conflict of interest statement

None of the authors has any potential conflict of interest.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Arunasiri Iddamalgoda
    • 1
    Email author
  • Quang Trong Le
    • 2
  • Kenichi Ito
    • 1
  • Kiyotaka Tanaka
    • 1
  • Hiroyuki Kojima
    • 1
  • Hiroshi Kido
    • 2
  1. 1.Department of Research and Development Ichimaru Pharcos Co. LtdMotosu CityJapan
  2. 2.Institute of Enzyme ChemistryUniversity of TokushimaTokushimaJapan

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