Archives of Dermatological Research

, Volume 298, Issue 7, pp 347–352 | Cite as

Association of HLA loci alleles and antigens in Saudi patients with vitiligo

  • Abdullah Abanmi
  • Fahad Al Harthi
  • Riyadh Al Baqami
  • Saleh Al Assaf
  • Abdulrahman Zouman
  • Misbahul Arfin
  • Mohammad Tariq
Original Paper

Abstract

HLA complex is composed of several closely linked loci, each containing several alleles, yielding a high expression of polymorphism. Vitiligo, a commonly acquired dermatological disorder, has been associated with different HLA antigens in different ethnic groups. In this study, HLA classes I (HLA-A, B, and C) and II (HLA-DR, DQ) antigens/alleles were analyzed in a group of 80 Saudi subjects consisting of vitiligo patients (40) and matched controls (40). The frequency of antigens of various HLA loci was tested using two-stage microcytotoxicity assays, while the frequency of alleles of HLA-DR was screened by polymerase chain reaction/sequence specific primers (PCR/SSP) method. The frequencies of HLA-B7, B15, Bw6, Cw6, Cw7, and DRB4*010101 were found to be significantly higher in vitiligo patients compared to controls [P = 0.029, 0.015, 0.033, 0.009, 0.043, and 0.015, respectively, with relative risk (RR) ≥ 3, etiologic fraction (EF) ≥ 0.4]. On the other hand, HLA-A9, B5, DQ1, and DRB3*010101 were significantly decreased in vitiligo patients compared to healthy Saudis [P = 0.008, 0.004, 0.028, and 0.04, respectively, with RR < 1 and preventive fraction (PF) < 0.5]. Among the patients, the highest allele frequency was noted for DRB4*010101(70%), while in controls it was for DRB3*010101 (72.5%). These results for antigens and allele frequency of various HLA Loci in vitiligo patients and control subjects suggested that HLA-B7, Bw6, Cw6, Cw7, and DRB4*010101 could be susceptible to vitiligo, while HLA-A9, B5, DQ1, and DRB3*010101 might be negatively associated with the development of vitiligo in Saudis.

Keywords

Vitiligo HLA Saudi 

References

  1. 1.
    Al-Fouzan A, al-Arbash M, Fouad F, Kaaba SA, Mousa MA, al-Harbi SA (1995) Study of HLA classI/IL and T lymphocyte subsets in Kuwaiti vitiligo patients. Eur J Immunogenet 22(2):209–213PubMedGoogle Scholar
  2. 2.
    Alper CA, Boenisch T, Watson L (1972) Genetic polymorphism in human glycine-rich beta glycoprotein. J Exp Med 135:68–80PubMedCrossRefGoogle Scholar
  3. 3.
    Amos DB, Pool P, Grier J (1980) HLA-A, HLA-B, HLA-C, and HLA-DR. In: Rose NR, Friedman H (eds) Manual of clinical immunology. American Society of Microbiology, Washington, DC, pp 978–986Google Scholar
  4. 4.
    Ando I, Chi HI, Nakagawa H, Otsuha F (1993) Difference in clinical features and HLA antigens between familial and non-familial vitiligo of non-segmental type. Br J Dermatol 129(4):408–410PubMedCrossRefGoogle Scholar
  5. 5.
    Arcos-Burgos M, Parodi E, Salgar M, Bedya E, Buile J, Jaramillo D (2002) Vitiligo: complex segregation and linkage disequilibrium analyses with respect to microsatellite loci spanning the HLA. Hum Genet 110:334–342PubMedCrossRefGoogle Scholar
  6. 6.
    Buc M, Busofa B, Hegyi E, Kolibasova K (1996) Vitiligo is associated with HLA-A2 and HLA-Dw7 in the Slovak populations. Folia Biol (Phraha) 42(1–2):23–25Google Scholar
  7. 7.
    Buc M, Fazekasova H, Cechova E, Hegyi E, Kolibasova K, Ferencik S (1998) Occurrence rates of HLA-DRB1, HLA-DQB1, and HLA-DPB1 alleles in patients suffering from vitiligo. Eur J Dermatol 8(1):13–15PubMedGoogle Scholar
  8. 8.
    Cui J, Harning R, Henn M, Bstryn JC (1992) Identification of pigment cell antigens defined by vitiligo antibodies. J Invest Dermatol 98(2):162–165PubMedCrossRefGoogle Scholar
  9. 9.
    Dominguez-Soto L, Hojyo-Tomoka T, Vega-Memije E, Arenas R, Cores-Franco R (1994) Pigmentary problems in the tropics. Dermatol Clin 12(4):777–784PubMedGoogle Scholar
  10. 10.
    Dunston GM, Halder RM (1990) Vitiligo is associated with HLA-DR4 in black patients. A preliminary report. Arch Dermatol 126(1):56–60PubMedCrossRefGoogle Scholar
  11. 11.
    Engleman EG, Grumet FC (1987) Influence of the HLA system on disease susceptibility. In: Fitz Patrick TB, Eisen AZ, et al (eds) Dermatology in general medicine, 3rd edn. McGraw-Hill, New York, pp 161–168Google Scholar
  12. 12.
    Finco O, Cuccia M, Martinetti M, Ruberto G, Orecchia G, Rabbiosi G (1991) Age of onset of vitiligo: relationship with HLA supratypes. Clin Genet 39(1):48–54PubMedCrossRefGoogle Scholar
  13. 13.
    Fishman P, Azizi E, Shoenfeld Y, Sredni B, Yecheskel G, Ferrone S, Zigelman R, Chaitchik S, Floro S, Djaldetti M (1993) Vitiligo autoantibodies are effective against melanoma. Cancer 72(8):2365–2369PubMedCrossRefGoogle Scholar
  14. 14.
    Foley LM, Lowe NJ, Misheloff E, Tiwari JL (1983) Association of HLA-DR4 with vitiligo. J Am Acad Dermatol 8(1):39–40PubMedCrossRefGoogle Scholar
  15. 15.
    Hann S-K, Nordlund JJ (2000) Clinic features of generalized vitiligo. In: Hann S-K, Nordlund JJ (eds) The vitiligo. Blackwell, Oxford, pp 39–42Google Scholar
  16. 16.
    Harning R, Cui J, Bystryn JC (1991) Relation between the incidence and level of pigment cell antibodies and disease activity in vitiligo. J Invest Dermatol 97(6):1078–1080PubMedCrossRefGoogle Scholar
  17. 17.
    Imanishi T, Akaza T, Kimura A, Tokunaga K, Gojobori (1992) T.Allele and haplotype frequencies for HLA and complement loci in various ethnic groups. In: Tsuji K, Aizawa M, Sasazuki T (eds) HLA 1991, vol 1. Oxford University Press, New York, pp 1065–1220Google Scholar
  18. 18.
    Le Poole IC, Das PK, Van den Wijngaard RM, Bos JD, Westerhof W (1993) Review of etiopathomechanism of vitiligo: a convergence theory. Exp Dermatol 2(4):145–153PubMedCrossRefGoogle Scholar
  19. 19.
    Lorini R, Orecchia G, Maritinetti M, Dugoujon JM, Cuccia M (1992) Autoimmunity in vitiligo: relationship with HLA, Gm and Km polymorphisms. Autoimmunity 11(4):255–260PubMedGoogle Scholar
  20. 20.
    Mattiuz PL, Ihde D, Piazza A, Ceppellini R, Bodmer WF(1970) New approaches to the population genetics and segregation analysis of the HLA system. In: Terasaki PI (ed) Histocompatibility testing. Munksgaard, KopenhagenGoogle Scholar
  21. 21.
    Metzker A, Zamir R, Gazit E, David M, Feurerman EJ (1980) Vitiligo and the HLA system. Dermatologica 160:100–105PubMedCrossRefGoogle Scholar
  22. 22.
    Naughton GK, Reggiardo D, Bystryn JC (1986) Correlation between vitiligo antibodies and extent of depigmentation in vitiligo. J Am Acad Dermatol 15(5 Pt 1):978–981PubMedCrossRefGoogle Scholar
  23. 23.
    Ollier W, Doyle P, Alonso A, Awad J, Williams E, Gill D, Welch S, Klouda P, Bacchus R, Festenstein H (1985) HLA polymorphisms in Saudi Arabs. Tissue Antigens 25(2):87–95PubMedCrossRefGoogle Scholar
  24. 24.
    Orecchia G, Perfetti L, Malagoli P, Borghini F, Kipervarg Y (1992) Vitiligo is associated with a significant increase in HLA-A30, Cw6, and DQw3, and a decrease in C4AQ0 in northern Italian patients. Dermatology 185(2):123–127PubMedCrossRefGoogle Scholar
  25. 25.
    Ortonne JP, Bose SK (1993) Vitiligo: where do we stand? Pigment Cell Res 6(2):61–72PubMedCrossRefGoogle Scholar
  26. 26.
    Porter RR (1983) Complement polymorphism. The major histocompatibility complex and associated disease: a speculation. Mol Biol Med 1:161–168PubMedGoogle Scholar
  27. 27.
    Savejgaard A, Platz P, Ryder LP (1983) HLA and disease 1982-A survey. Immunol Rev 70:193–218CrossRefGoogle Scholar
  28. 28.
    Schallreuter KU, Levenig C, Berger J (1991) Vitiligo and cutaneous melanoma. A case study. Dermatologica 183(4):239–245Google Scholar
  29. 29.
    Sheth KV, Edwards JA, Godwin JT (1985) Study of the HLA gene and antigen frequency from a Saudi Arabian Hospital. Tissue Antigens 25(3):156–162PubMedCrossRefGoogle Scholar
  30. 30.
    Tastan HB, Akar A, Orkunoglu FE, Arca E, Inal A (2004) Association of class I antigens and HLA class II alleles with vitiligo in a Turkish population. Pigment Cell Res 17(2):181–184PubMedCrossRefGoogle Scholar
  31. 31.
    Venkataram MN, White AG, Leeny WA, al Suwaid AR, Daar AS (1995) HLA antigens in Omani patients with vitiligo. Clin Exp Dermatol 20(1):35–37PubMedCrossRefGoogle Scholar
  32. 32.
    Venneker GT, de Waal LP, Westerhof W, D’Amaro J, Schreuder GM, Asghar SS (1993) HLA associations in vitiligo patients in the Dutch population. Dis Markers 11(4):187–190PubMedGoogle Scholar
  33. 33.
    White AG, Lehny W, Kuchipudi P, Varghese M, Al Riyami H, Al Hashmi S, Daar AS (1999) Histocompatibility antigens in Omanis: comparison with other Gulf populations and implications for disease association. Ann Saudi Med 19(3):193–196PubMedGoogle Scholar
  34. 34.
    Zamani M, Spaepen M, Asghar SS, Huang C, Westerhof W, Nieweboer-Krobotova L, Cassiman JJ (2001) Linkage and association of HLA class II genes with vitiligo in Dutch population. BR J Dermatol 145(1): 90–94PubMedCrossRefGoogle Scholar
  35. 35.
    Zhang XJ, Liu HS, Liang YH, Sun LD, Wang JY, Yang S, Liu JB, Gao M, He PP, Cui Y, Yang Q (2004) Association of HLA class I alleles with vitiligo in Chinese Hans. J Dermatol Sci 35(2):165–168PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Abdullah Abanmi
    • 1
  • Fahad Al Harthi
    • 1
  • Riyadh Al Baqami
    • 1
  • Saleh Al Assaf
    • 1
  • Abdulrahman Zouman
    • 1
  • Misbahul Arfin
    • 1
  • Mohammad Tariq
    • 1
  1. 1.Department of DermatologyArmed Forces HospitalRiyadhSaudi Arabia

Personalised recommendations