Archives of Dermatological Research

, Volume 298, Issue 3, pp 107–112

Systematic evaluation of association between the microsomal glutathione S-transferase 2 common variation and psoriasis vulgaris in Chinese population

  • Sen Yang
  • Kai-Lin Yan
  • Xue-Jun Zhang
  • Feng-Li Xiao
  • Xing Fan
  • Min Gao
  • Yong Cui
  • Peng-Guang Wang
  • Guo-Long Zhang
  • Liang-Dan Sun
  • Zhi-Min Wang
  • Da-Zhi Wang
  • Kai-Yue Zhang
  • Wei Huang
  • Jian-Jun Liu
Original Paper

Abstract

Several recent studies have demonstrated the possible involvement of the microsomal glutathione S-transferase 2 (MGST2) gene in the pathogenesis of psoriasis. The objectives of this work are to determine whether the genetic polymorphisms of the MGST2 gene were associated with an increased risk of psoriasis in Chinese patients. We first characterized the linkage disequilibrium pattern within MGST2 and identified single-nucleotide polymorphisms (SNPs) for tagging common genetic variants. Genotype- and haplotype-based analyses were then performed by genotyping the Tag SNPs in a large-scale sample of cases and controls. We characterized the linkage disequilibrium pattern within MGST2 using 12 densely distributed SNPs and identified 6 SNPs for tagging common genetic variants. We then performed an association analysis by genotyping the six SNPs in 552 cases and 384 controls, but none of the genotype- and haplotype-based analyses revealed significant evidence for association. We also performed family-based association analysis by genotyping the six SNPs in 95 trios; no evidence for association was identified. Our comprehensive genetic analysis of MGST2 common variants in a large Chinese sample of psoriasis did not provide any supporting evidence for MGST2 to be the susceptibility gene within the PSORS9 locus.

Keywords

Association analysis Haplotype Linkage disequilibrium MGST2 Psoriasis vulgaris TDT 

Reference

  1. 1.
    Asumalahti K, Laitinen T, Lahermo P, Suomela S, Itkonen-Vatjus, Jansen C, Karvoven J, Karvoven SL, Reunala T, Snellman E, Uurasmaa T, Saarialho-Kere U, Kere J (2003) Psoriasis susceptibility locus on 18p revealed by genome scan in Finnish families not associated with PSORS1. J Invest Dermatol 121:735–740PubMedCrossRefGoogle Scholar
  2. 2.
    Barrett JC, Fry B, Maller J, Daly MJ (2005) Haploview: analysis and visualization of LD and haplotype maps. Bioinformatics 21:263–265PubMedCrossRefGoogle Scholar
  3. 3.
    Bornman L, Campbell SJ, Fielding K, Bah B, Sillah J, Gustafson P, Maneh K, Lisse I, Allen A, Sirugo G, Sylla A, Aaby P, McAdam KP, Bah-Snow O, Bennett S, Lienhardt C, Hill AV (2004) Vitamin D receptor polymorphisms and susceptibility to tuberculosis in West Africa: a case–control and family study. J Infect Dis 190:1631–1641PubMedCrossRefGoogle Scholar
  4. 4.
    Capon F, Novelli G, Semprini S, Clementi M, Nudo M, Vultaggio P, Mazzanti C, Gobello T, Botta G, Fabrizi G, Dallapiccola B (1999) Searching for psoriasis susceptibility genes in Italy: genome scan and evidence for a new locus on chromosome 1. J Invest Dermatol 112:32–35PubMedCrossRefGoogle Scholar
  5. 5.
    Ding K, Zhou K, He F, Shen Y (2003) LDA—a java-based linkage disequilibrium analyzer. Bioinformatics 19:2147–2148PubMedCrossRefGoogle Scholar
  6. 6.
    Gauderman WJ (2002) Sample size requirements for association studies of gene-gene interaction. Am J Epidemiol 155:478–484PubMedCrossRefGoogle Scholar
  7. 7.
    Iselius L, Williams WR (1984) The mode of inheritance of psoriasis: evidence for a major gene as well as a multifactorial component and its implication for genetic counseling. Hum Genet 68:73–76PubMedCrossRefGoogle Scholar
  8. 8.
    Iversen L, Deleuran B, Hoberg AM, Kraggballe K (1996) LTA4 hydrolase in human skin: decreased activity but normal concentration in lesional psoriatic skin. Arch Dermatol Res 288:217–241PubMedCrossRefGoogle Scholar
  9. 9.
    Jakobsson PJ, Mancini JA, Ford-Hutchinson AW (1996) Identification and characterization of a novel human microsomal glutathione S-Transferase with leukotriene C4 synthase activity and significant sequence identity to 5-Lipoxygenase-activating protein and leukotriene C4 synthase. J Biol Chem 271:22203–22210PubMedCrossRefGoogle Scholar
  10. 10.
    Karason A, Gudjonsson JE, Jonsson HH, Hauksson VB, Runarsdottir EH, Stefansson K, Valdimarsson H, Gulcher JR (2005) Genetics of psoriasis in Iceland: evidence for linkage of subphenotypes to distinct loci. J Invest Dermatol 124:1177–1185PubMedCrossRefGoogle Scholar
  11. 11.
    Lee YA, Ruschendorf F, Windemuth C, Schmitt-Egenolf M, Stadelmann A, Nurnberg G, Stander M, Wienker TF, Reis A, Traupe H (2000) Genome wide scan in German families reveals evidence for a novel psoriasis-susceptibility locus on chromosome 19p13. Am J Hum Genet 67:1020–1024PubMedCrossRefGoogle Scholar
  12. 12.
    Matthews D, Fry L, Powles A, Weber J, McCarthy M, Fisher E, Davies K, Williamson R (1996) Evidence that a locus for familial psoriasis maps to chromosome 4q. Nat Genet 14:231–233PubMedCrossRefGoogle Scholar
  13. 13.
    Miller S, Dykes D, Polesky H (1988) A simple salting out procedure for extraction of high molecular weight DNA from human nucleated cells. Nucleic Acids Res 16:1215PubMedCrossRefGoogle Scholar
  14. 14.
    Nair RP, Henseler T, Jenisch S, Stuart P, Bichakjian CK, Lenk W, Westphal E, Guo SW, Christophers E, Voorhees JJ, Elder JT (1997) Evidence for two psoriasis susceptibility loci (HLA and 17q) and two novel candidate regions (16q and 20p) by genome-wide scan. Hum Mol Genet 6:1349–1356PubMedCrossRefGoogle Scholar
  15. 15.
    Nevitt GJ, Hutchinson PE (1996) Psoriasis in the community: prevalence, severity and patients’ beliefs and attitudes towards the disease. Br J Dermatol 135:533–537PubMedCrossRefGoogle Scholar
  16. 16.
    Reich DE, Cargill M, Bolk S, Ireland J, Sabeti PC, Richter DJ, Lavery T, Kouyoumjian R, Farhadian SF, Ward R, Lander ES (2001) Linkage disequilibrium in the human genome. Nature 411:199–204PubMedCrossRefGoogle Scholar
  17. 17.
    Sagoo GS, Tazi-Ahnini R, Barker JW, Elder JT, Nair RP, Samuelsson L, Traupe H, Trembath RC, Robinson DA, Iles MM (2004) Meta-analysis of genome-wide studies of psoriasis susceptibility reveals linkage to chromosomes 6p21 and 4q28-q31 in Caucasian and Chinese Hans population. J Invest Deramtol 122:1401–1405CrossRefGoogle Scholar
  18. 18.
    Samuelsson L, Enlund F, Torinsson A, Yhr M, Inerot A, Enerback C, Wahlstrom J, Swanbeck G, Martinsson T (1999) A genome-wide search for genes predisposing to familial psoriasis by using a tratification approach. Hum Genet 105:523–529PubMedCrossRefGoogle Scholar
  19. 19.
    Seyger MM, van Pelt JP, van den Born J, Laijnhouwers MA, de Jong EM (1997) Epicutaneous application of leukotriene B4 induces patterns of tenascin and a heparan sulfate proteoglycan epitope that are typical for psoriatic lesions. Arch Dermatol Res 289:331–336PubMedCrossRefGoogle Scholar
  20. 20.
    Sham PC, Curtis D (1995) An extended transmission disequilibrium test (TDT) for multi-allele marker loci. Ann Hum Genet 59:323–336PubMedCrossRefGoogle Scholar
  21. 21.
    Shao CG (1996) The prevalence, prevention and treatment of psoriasis in China. Chin J Dermatol 29:75–76Google Scholar
  22. 22.
    Simons RD (1949) Additional studies on psoriasis in the tropics and in the starvation camps. J Invest Dermatol 12:285–294PubMedGoogle Scholar
  23. 23.
    Stephens M, Smith NJ, Donnelly P (2001) A new statistical method for haplotype reconstruction from population data. Am J Hum Genet 68:978–989PubMedCrossRefGoogle Scholar
  24. 24.
    Tomfohrde J, Silverman A, Barnes R, Fernandez-Vina MA, Young M, Lory D, Morris L, Wuepper KD, Stastny P, Menter A (1994) Gene for familial psoriasis susceptibility mapped to the distal end of human chromosome 17q. Science 264:1141–1145PubMedCrossRefGoogle Scholar
  25. 25.
    Trembath RC, Clough RL, Rosbotham JL, Jones AB, Camp RD, Frodsham A, Browne J, Barber R, Terwilliger J, Lathrop GM, Barker JN (1997) Identification of a major susceptibility locus on chromosome 6p and evidence for further disease loci revealed by a two stage genome-wide search in psoriasis. Hum Mol Genet 6:813–820PubMedCrossRefGoogle Scholar
  26. 26.
    Tzschach A, Hoffmann K, Hoeltzenbein M, Bache I, Tommerup N, Bommer C, Körner H, Kalscheuer V, Ropers HH. 2005. Molecular characterization of a balanced chromosome translocation in psoriasis vulgaris. Clin Genet 69:189–193CrossRefGoogle Scholar
  27. 27.
    Veal CD, Clough RL, Barber RC, Mason S, Tillman D, Ferry B, Jones AB, Ameen M, Balendran N, Powis SH, Burden AD, Barker JN, Trembath RC (2001) Identification of a novel psoriasis susceptibility locus at 1p and evidence of epistasis between PSORS1 and candidate loci. J Med Genet 38:7–13PubMedCrossRefGoogle Scholar
  28. 28.
    Wedi B, Kapp A (2001) Pathophysiological role of leukotrienes in dermatological diseases. BioDrugs 15:729–743PubMedCrossRefGoogle Scholar
  29. 29.
    Yan KL, Zhang XJ, Wang ZM, Yang S, Zhang GL, Wang J, Xiao FL, Gao M, Cui Y, Chen JJ, Fan X, Sun LD, Xia Q, Zhang KY, Niu ZM, Xu SJ, Tzschach A, Ropers H, Huang W, Liu JJ (2006) A novel MGST2 non-synonymous mutation in a Chinese pedigree with psoriasis vulgaris. J Invest Dermatol 126:1003–1005PubMedCrossRefGoogle Scholar
  30. 30.
    Yui Yip S (1984) The prevalence of psoriasis in the mongoloid race. J Am Acad Dermatol 10:965–968CrossRefGoogle Scholar
  31. 31.
    Zhang XJ, He PP, Li M, He CD, Yan KL, Cui Y, Yang S, Zhang KY, Gao M, Chen JJ, Li CR, Jin L, Chen HD, Xu SJ, Huang W (2004) Seven novel mutations of the ADAR gene in Chinese families and sporadic patients with dyschromatosis symmetrica hereditaria (DSH). Hum Mutat 23:629–30PubMedCrossRefGoogle Scholar
  32. 32.
    Zhang XJ, He PP, Wang ZX, Zhang J, Li YB, Wang HY, Wei SC, Chen SY, Xu SJ, Jin L, Yang S, Huang W (2002) Evidence for a major psoriasis susceptibility locus at 6p21 (PSORS1) and a novel candidate region at 4q31 by genome-wide scan in Chinese Hans. J Invest Dermatol 119:1361–1366PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Sen Yang
    • 1
    • 2
  • Kai-Lin Yan
    • 1
    • 2
  • Xue-Jun Zhang
    • 1
    • 2
  • Feng-Li Xiao
    • 1
    • 2
  • Xing Fan
    • 1
    • 2
  • Min Gao
    • 1
    • 2
  • Yong Cui
    • 1
    • 2
  • Peng-Guang Wang
    • 1
    • 2
  • Guo-Long Zhang
    • 1
    • 2
  • Liang-Dan Sun
    • 1
    • 2
  • Zhi-Min Wang
    • 3
    • 4
  • Da-Zhi Wang
    • 3
  • Kai-Yue Zhang
    • 3
  • Wei Huang
    • 3
  • Jian-Jun Liu
    • 1
    • 2
    • 5
  1. 1.Institute of Dermatology and Department of Dermatology at No.1 HospitalAnhui Medical UniversityHefeiPeople’s Republic of China
  2. 2.The Key Laboratory of Gene Resource Utilization for Severe DiseasesMinistry of EducationHefeiPeople’s Republic of China
  3. 3.Chinese National Human Genome Center at ShanghaiShanghaiPeople’s Republic of China
  4. 4.State Key Laboratory of Genetic Engineering and Center for Anthropological Studies, School of Life SciencesFudan UniversityShanghaiPeople’s Republic of China
  5. 5.Genome Institute of SingaporeSingaporeSingapore

Personalised recommendations